Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in<i>Caenorhabditis elegans</i>

Oren‐Suissa, Meital; Gattegno, Tamar; Kravtsov, Veronika; Podbilewicz, Benjamin

doi:10.1534/genetics.116.196386

Cited by 55 publications

(94 citation statements)

References 106 publications

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“…Recently, an important role of autofusion during axonal regeneration was demonstrated in the PLM sensory neurons of C. elegans, a process mediated by the fusogen EFF-1 Neumann et al, 2015). In the PVD, EFF-1 is not necessary for dendrite reconnection following injury (Oren-Suissa et al, 2017). In the PVD dendrites, this role is fulfilled by the EFF-1 paralog AFF-1.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to eff-1, aff-1 mutants have no evident morphological phenotypes in the PVD and aff-1 expression has not been detected in this neuron ( Fig. S2; Oren-Suissa et al, 2017); moreover, when AFF-1 is ectopically expressed in the PVD in an eff-1 mutant background it does not retract excess branching, demonstrating that it is unable to Percentage of defects in self-avoidance in 100 μm of length around PVD cell body at L4 stage in different genotypes; n≥7. Defective self-avoidance increases in daf-2 mutants in a daf-16-dependent manner.…”

Section: Age-dependent Dendrite Remodeling Can Be Modulated By Eff-1mentioning

confidence: 91%

“…EFF-1 and its paralog C26D10.7) might also participate in the repair process. Our recent work has shown that non-autonomous AFF-1 derived from the epidermal seam cells can mediate terminal branch fusion in young animals (Oren-Suissa et al, 2017). Interestingly, of the fusogens identified in C. elegans none of them is independently required for primary dendrite merger after injury, suggesting redundancy or the activity of an unidentified fusogen (OrenSuissa et al, 2017).…”

Section: Rejuvenation Of Fusion Potential Of Cut Dendrites By Daf-2 Mmentioning

confidence: 99%

“…Thus, this fusogen maintains the structural units by trimming excess branching during normal developmental arborization (Oren-Suissa et al, 2010). AFF-1, a paralog of EFF-1, is a second C. elegans fusogen displaying a more restricted tissue distribution pattern (Avinoam and Podbilewicz, 2011;Sapir et al, 2007); AFF-1 was not found to be involved in PVD remodeling during development (Oren-Suissa et al, 2017). AFF-1 and EFF-1 fusion proteins also auto-fuse epithelial and myoepithelial cells to form tubes and reshape glial cells (Procko et al, 2011;Rasmussen et al, 2008;Soulavie and Sundaram, 2016;Stone et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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AFF-1 fusogen can rejuvenate the regenerative potential of adult dendritic trees via self-fusion

2017

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The aging brain undergoes structural changes that affect brain homeostasis, neuronal function and consequently cognition. The complex architecture of dendritic arbors poses a challenge to understanding age-dependent morphological alterations, behavioral plasticity and remodeling following brain injury. Here, we use the PVD polymodal neurons of C. elegans as a model to study how aging affects neuronal plasticity. Using confocal live imaging of C. elegans PVD neurons, we demonstrate age-related progressive morphological alterations of intricate dendritic arbors. We show that mutations in daf-2, which encodes an insulin-like growth factor receptor ortholog, fail to inhibit the progressive morphological aging of dendrites and do not prevent the minor decline in response to harsh touch during aging. We uncovered that PVD aging is characterized by a major decline in the regenerative potential of dendrites following experimental laser dendrotomy. Furthermore, the remodeling of transected dendritic trees by AFF-1-mediated self-fusion can be restored in old animals by daf-2 mutations, and can be differentially re-established by ectopic expression of the fusion protein AFF-1. Thus, ectopic expression of the fusogen AFF-1 in the PVD and mutations in daf-2 differentially rejuvenate some aspects of dendritic regeneration following injury.

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Section: Discussionmentioning

confidence: 99%

Section: Age-dependent Dendrite Remodeling Can Be Modulated By Eff-1mentioning

confidence: 91%

Section: Rejuvenation Of Fusion Potential Of Cut Dendrites By Daf-2 Mmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AFF-1 fusogen can rejuvenate the regenerative potential of adult dendritic trees via self-fusion

2017

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“…Хотя Догель не занимался слиянием перерезанных концов волокон, он все-таки полагал, что при контакте концов анастомозов происходит переплетение фибрилл и совершается обмен между входящими в состав отростков [8]. Подобные цитоплазматические слияния аксонов и дендритов уже описаны в литературе неоднократно при перерезке нейритов [41,42]. Во-вторых, синцитиальные цитоплазматические анастомозы волокон могут формироваться с помощью их контактов и слияния «бок-в-бок» с помощью их синцитиального слияния с соседними клетками.…”

Section: рисunclassified

The conception of the reticular organization of the nervous tissue of Alexander Dogiel

Os¹,

Markov²

2018

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Резюме: В этом году исполняется 95 лет со дня смерти Александра Станиславовича Догеля, профессора, знаменитого нейрогистолога, прославившего нейрогистологию неповторимыми суправитальными исследованиями нервной системы с помощью красителя метиленового синего. Основополагающие работы Догеля равны по значению трудам Рамон-и-Кахаля, Ретциуса и других общепризнанных неврологов. Принципиальное отличие трудов Догеля от работ великого Рамон-и-Кахаля состоит в том, что ему впервые и безусловно удалось доказать синцитиальную цитоплазматическую связь тел и отростков в системе нейронов. Его работы окончательно подтвердили данные Вагнера, Sosa-Sosa, Кульчицкого и многих других о связи синцитиальных межнейронных комиссур. Он впервые получил бесспорные картины паутиннотонких нервных сетей -настоящий ретикулум. Авторы статьи проанализировали изображения препаратов выдающихся сторонников нейронной теории (Ван Гехухтена, Рамон-и-Кахаля и Ретциуса), подтверждающие возможность синцитиальной связи в нервной системе и доказательств ретикулярной теории. С помощью прижизненных исследований в культуре ткани авторам также удалось доказать возможность синцитиального слияния нервных отростков, появление двуядерных клеток и формирования элементов нервного ретикулума.Ключевые слова: Догель, ретикулярная теория нейронов, нервный ретикулум Summary: 2017 marks the 95th year of the death of Alexander Stanislavovich Dogel, a professor and a famous neurohistologist who added lustre to neurohistology by his unique supravital studies of the nervous system by means of methylene blue. Dogel's basis works are equal in their significance to those of Ramon-y-Kajal, Retzius and other universally acknowledged neurologists. The fundamental difference between the works of Dogel and the works of the great Ramon-y-Kajal is that he was the first to indisputably prove the syncytial and cytoplasmic connection between the soma and the dendrites in the neuron system. His works decisively confirmed the data obtained by Wagner, Sosa-Sosa, Kulchitsky and many others on the connection between syncytial commissural interneurons. He was the first to get apparent images of cobweb-like neural nets -a real reticulum. We have also managed to find original pictures of preparates used by the prominent adherents of the neuron theory -A. Van Gehuchten, Ramon-y-Kajal, Retzius -that confirm the possibility of syncytial connectivity in the neural system and arguments of the reticular theory. The intravital own studies of the tissue allowed the authors of the article to prove the possibility of syncytial fusion of nerve dendrites, emergence of binucleate cells and formation of the nerve reticulum elements.

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Programmed cell fusion in development and homeostasis

Iosilevskii

Podbilewicz

2021

Current Topics in Developmental Biology

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Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion inCaenorhabditis elegans

Abstract: Neurons in the CNS have limited regenerative ability. Genetic pathways have been identified for axonal regeneration, but few studies exist on dendrites...

Cited by 55 publications

References 106 publications

AFF-1 fusogen can rejuvenate the regenerative potential of adult dendritic trees via self-fusion

AFF-1 fusogen can rejuvenate the regenerative potential of adult dendritic trees via self-fusion

The conception of the reticular organization of the nervous tissue of Alexander Dogiel

Programmed cell fusion in development and homeostasis

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