“…In vitro reconstitution of functional 30S and 50S ribosomal subunits is a self‐assembly process (Nierhaus, 2004; Talkington et al ., 2005). This does not, however, preclude the involvement of additional factors in vivo (Alix, 1993). Indeed extra‐ribosomal factors have been reported, such as the chaperone DnaK (El Hage and Alix, 2004), RbfA (Datta et al ., 2007), RimM (Suzuki et al ., 2007), RNA helicases DeaD/CsdA, SrmB (Iost and Dreyfus, 2006; Peil et al ., 2008) and RhlE (Jain, 2008), GTPases (Era (Sharma et al ., 2005), Der (Hwang and Inouye, 2006), RsgA (Himeno et al ., 2004), CgtA E (Jiang et al ., 2006; 2007), post‐transcriptional rRNA modifying enzymes [(RluD (Gutgsell et al ., 2005), RrmJ (Hager et al ., 2004), YrdC (Kaczanowska and Rydén‐Aulin, 2005)], and post‐translational ribosomal protein modifying enzymes [PrmB (Lhoest and Colson, 1981) and RimJ (Roy‐Chaudhuri et al ., 2008)].…”