Extrinsic Factors in Ribosome Assembly

Alix, Jean-Hervé

doi:10.1007/978-1-4615-2407-6_17

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…In vitro reconstitution of functional 30S and 50S ribosomal subunits is a self‐assembly process (Nierhaus, 2004; Talkington et al ., 2005). This does not, however, preclude the involvement of additional factors in vivo (Alix, 1993). Indeed extra‐ribosomal factors have been reported, such as the chaperone DnaK (El Hage and Alix, 2004), RbfA (Datta et al ., 2007), RimM (Suzuki et al ., 2007), RNA helicases DeaD/CsdA, SrmB (Iost and Dreyfus, 2006; Peil et al ., 2008) and RhlE (Jain, 2008), GTPases (Era (Sharma et al ., 2005), Der (Hwang and Inouye, 2006), RsgA (Himeno et al ., 2004), CgtA E (Jiang et al ., 2006; 2007), post‐transcriptional rRNA modifying enzymes [(RluD (Gutgsell et al ., 2005), RrmJ (Hager et al ., 2004), YrdC (Kaczanowska and Rydén‐Aulin, 2005)], and post‐translational ribosomal protein modifying enzymes [PrmB (Lhoest and Colson, 1981) and RimJ (Roy‐Chaudhuri et al ., 2008)].…”

Section: Introductionmentioning

confidence: 99%

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†

Refaii

Alix²

2009

Molecular Microbiology

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SummaryIn Escherichia coli strains carrying null mutations in either the dnaK or dnaJ genes, the late stages of 30S and 50S ribosomal subunit biogenesis are slowed down in a temperature-dependent manner. At high temperature (44°C), 32S and 45S particles (precursors to 50S subunits) and 21S particles (precursors to 30S subunits) accumulate. The latter are shown by 3Ј5Ј rapid amplification of cDNA ends analysis to contain unprocessed or partially processed 16S ribosomal RNA at the 5Ј end, but the 3Ј end was never processed. This implies that maturation of 16S ribosomal RNA starts at the 5Ј-terminus, and that the 3Ј-terminus is only trimmed at a later step. At normal temperatures (30°C-37°C), ribosome assembly in both mutants is not arrested but is significantly delayed, as shown by pulse-chase analysis. Assembly defects are partially compensated by an overexpression of other heat-shock proteins, which occurs in the absence of their negative regulator DnaK, or by a plasmid-driven overexpression of GroES/GroEL, suggesting the involvement of a network of chaperones in ribosome biogenesis.

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Section: Introductionmentioning

confidence: 99%

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†

Refaii

Alix²

2009

Molecular Microbiology

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“…Extrinsic factors are likely to be involved in assembly of bacterial ribosome subunits (Alix 1993; for a recent extensive review of the 30S assembly, see Culver 2003). Such factors should be necessary to circumvent the heat-dependent conformational change of ribosomal precursor intermediates required during in vitro reconstitution of ribosomes (Nierhaus 1991;Williamson 2003).…”

Section: Introductionmentioning

confidence: 99%

DnaK-facilitated ribosome assembly inEscherichia colirevisited

Alix

Nierhaus

2003

RNA

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Assembly helpers exist for the formation of ribosomal subunits. Such a function has been suggested for the DnaK system of chaperones (DnaK, DnaJ, GrpE). Here we show that 50S and 30S ribosomal subunits from an Escherichia coli dnaK-null mutant (containing a disrupted dnaK gene) grown at 30°C are physically and functionally identical to wild-type ribosomes. Furthermore, ribosomal components derived from mutant 30S and 50S subunits are fully competent for in vitro reconstitution of active ribosomal subunits. On the other hand, the DnaK chaperone system cannot circumvent the necessary heat-dependent activation step for the in vitro reconstitution of fully active 30S ribosomal subunits. It is therefore questionable whether the requirement for DnaK observed during in vivo ribosome assembly above 37°C implicates a direct or indirect role for DnaK in this process.

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“…Various genetic and biochemical data indicate that extra-ribosomal factors or chaperones are likely to be involved in the biogenesis of the E. coli ribosome in vivo (Alix 1993); for example, total reconstitution of active prokaryotic 30S and 50S ribosomal subunits is possible in vitro, but only under conditions that are far from physiological. For these reasons we have undertaken a study of the possible role in ribosome formation of the most abundant chaperone in E. coli, the product of the heat-shock gene dnaK.…”

Section: Introductionmentioning

confidence: 99%

The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli

2001

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It has been shown that in Escherichia coli the chaperone DnaK is necessary for the late stages of 50S and 30S ribosomal subunit assembly in vivo. Here we focus on the roles of other HSPs (heat-shock proteins), including the chaperonin GroEL, in addition to DnaK, in ribosome biogenesis at high temperature. GroEL is shown to be required for the very late 45S-->50S step in the biogenesis of the large ribosome subunit, but not for 30S assembly. Interestingly, overproduction of GroES/GroEL can partially compensate for a lack of DnaK/DnaJ at 44 degrees C.

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Extrinsic Factors in Ribosome Assembly

Cited by 9 publications

References 47 publications

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†

DnaK-facilitated ribosome assembly inEscherichia colirevisited

The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli

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Extrinsic Factors in Ribosome Assembly

Cited by 9 publications

References 47 publications

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ†

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ†

DnaK-facilitated ribosome assembly inEscherichia colirevisited

The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli

Contact Info

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Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†

Ribosome biogenesis is temperature‐dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ^†