Extremophiles and biotechnology: current uses and prospects

Coker, James

doi:10.12688/f1000research.7432.1

Cited by 165 publications

(98 citation statements)

References 67 publications

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“…The most prominent sectors of the enzyme market are glycosyl hydrolases, lipases, proteases, the biomining/bioleaching, and biofuel sectors (Barnard et al. , Coker ). Accordingly, extremophilic microalgae have the potential to play an important role in the prospective of commercial exploitation of microalgae‐based biofuels, bioproducts, and agriculture (Varshney et al.…”

Section: Products and Applications Of Extremophile Microalgaementioning

confidence: 99%

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Extremophile Microalgae: the potential for biotechnological application

Malavasi

Soru

Cao

2020

Journal of Phycology

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Microalgae are photosynthetic microorganisms that use sunlight as an energy source, and convert water, carbon dioxide, and inorganic salts into algal biomass. The isolation and selection of microalgae, which allow one to obtain large amounts of biomass and valuable compounds, is a prerequisite for their successful industrial production. This work provides an overview of extremophile algae, where their ability to grow under harsh conditions and the corresponding accumulation of metabolites are addressed. Emphasis is placed on the high‐value products of some prominent algae. Moreover, the most recent applications of these microorganisms and their potential exploitation in the context of astrobiology are taken into account.

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Section: Products and Applications Of Extremophile Microalgaementioning

confidence: 99%

“…Some recent reviews have discussed extremophile microalgae including their products and applications (Mandal and Rath , Raddadi et al. , Coker , Amoozegar et al. ) as well as the field of astrobiology (Chyba and Hand , Martins et al.…”

mentioning

confidence: 99%

Extremophile Microalgae: the potential for biotechnological application

Malavasi

Soru

Cao

2020

Journal of Phycology

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“…Deste modo, alguns autores optam pelo termo "bactéria extremófila", outros por "arquea", no entanto o modo de se referir a estes micro-organismos é somente um detalhe ao observarmos tamanha a capacidade de se desenvolverem otimamente em ambientes considerados inóspitos para grande parte dos seres vivos. Na indústria, seja ela alimentícia, de limpeza, têxtil, de mineração, na área da genética ou mesmo em outros setores, o emprego das arqueas e/ou seus metabólitos podem atuar de forma específica e fundamental, otimizando os processos e reduzindo o custo e tempo demandado para produção [5].…”

Section: Introductionunclassified

Abordagem das bactérias extremófilas em livros didáticos de biologia do ensino médio

Santos

Braga

2019

Sci. Plena

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Os livros didáticos são os instrumentos de ensino mais acessíveis aos professores nas escolas, sendo primordial que os conteúdos possam ser contemplados da forma mais ampla possível. O tema arqueas, merece ser pontuado nesses materiais, especialmente pelos recentes avanços tecnológicos que possibilitam o emprego de tais micro-organismos em diversas áreas. Neste sentido, foram analisadas oito obras didáticas de Biologia do Ensino Médio segundo os aspectos gerais de Coerência e Conceitos, Quantificação e Interdisciplinaridade e Contextualização das bactérias extremófilas, tendo por base o guia do Programa Nacional do Livro Didático do Ministério da Educação. Os critérios "aplicações biotecnológicas e adaptações bioquímicas" foram os que apresentam as maiores defasagens em relação à abordagem do assunto, na maioria das obras didáticas. No geral, somente uma obra teve boa avaliação em todos os aspectos analisados. Quanto ao critério "coerência entre fundamentação teórica e, imagens, textos e exercícios propostos", todas as obras apresentaram-se satisfatórias. Portanto, apesar de as discussões sobre esse tema serem de suma importância ao contexto atual, as obras apresentam deficiências técnicas, em especial no que tange às aplicações biotecnológicas.Palavras-chave: Archaea, ensino de microbiologia, procariontes extremófilos.Textbooks are the most accessible teaching tools for teachers in schools, and it is essential that the contents can be contemplated in the widest possible way. The subject archaea deserves to be punctuated in these materials, especially by the recent technological advances that allow the use of such microorganisms in several areas. In this sense, eight didactic works of Teaching Biology were analyzed according to the general aspects of Coherence and Concepts, Quantification and Interdisciplinarity and Contextualization of the extremophilic bacteria, based on the guide of the National Program of Didactic Book of the Ministry of Education. The criteria "biotechnological applications and biochemical adaptations" were the ones with the greatest lags in relation to the subject approach in most didactic works. In general, only one work had good evaluation in all aspects analyzed. As to the criterion "coherence between theoretical foundation and images, texts and proposed exercises", all works were satisfactory. Therefore, although the discussions on this subject are of the utmost importance to the current context, the works present technical deficiencies, especially in what concerns the biotechnological applications.

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“…[3][4][5] For the purpose of fulfilling a wide variety of industrial needs, protease-producing bacteria are constantly being sought from different extreme environments such as glaciers, deep sea, saline habitats, mangroves, solvent-contaminated habitats, and swamps. [6][7][8][9][10][11] Swamps are forested freshwater wetlands on inundated soils that constitute unique natural environments. The swamps soils are rich in nutrients due to the leaf litter fall, and their high carbon content supports the growth and survival of a variety of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Novel extremophilic proteases from Pseudomonas aeruginosa M211 and their application in the hydrolysis of dried distiller's grain with solubles

Flores-Fernández

Cárdenas-Fernández

Dobrijevic

et al. 2018

Biotechnology Progress

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Proteases are the most important group of industrial enzymes and they can be used in several fields including biorefineries for the valorization of industrial byproducts. In this study, we purified and characterized novel extremophilic proteases produced by a Pseudomonas aeruginosa strain isolated from Mauritia flexuosa palm swamps soil samples in Peruvian Amazon. In addition, we tested their ability to hydrolyze distillers dried grains with solubles (DDGS) protein. Three alkaline and thermophilic serine proteases named EI, EII, and EIII with molecular weight of 35, 40, and 55 kDa, respectively, were purified. EI and EIII were strongly inhibited by EDTA and Pefabloc being classified as serine‐metalloproteases, while EII was completely inhibited only by Pefabloc being classified as a serine protease. In addition, EI and EII exhibited highest enzymatic activity at pH 8, while EIII at pH 11 maintaining almost 100% of it at pH 12. All the enzymes demonstrated optimum activity at 60°C. Enzymatic activity of EI was strongly stimulated in presence of Mn2+ (6.9‐fold), EII was stimulated by Mn2+ (3.7‐fold), while EIII was slightly stimulated by Zn2+, Ca2+, and Mg2+. DDGS protein hydrolysis using purified Pseudomonas aeruginosa M211 proteases demonstrated that, based on glycine released, EIII presented the highest proteolytic activity toward DDGS. This enzyme enabled the release 63% of the total glycine content in wheat DDGS protein, 2.2‐fold higher that when using the commercial Pronase®. Overall, our results indicate that this novel extremopreoteases have a great potential to be applied in DDGS hydrolysis. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2728, 2019

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Extremophiles and biotechnology: current uses and prospects

Cited by 165 publications

References 67 publications

Extremophile Microalgae: the potential for biotechnological application

Extremophile Microalgae: the potential for biotechnological application

Abordagem das bactérias extremófilas em livros didáticos de biologia do ensino médio

Novel extremophilic proteases from Pseudomonas aeruginosa M211 and their application in the hydrolysis of dried distiller's grain with solubles

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