Extreme conservation of miRNA complements in opisthorchiids

Ovchinnikov, Vladimir; Mordvinov, Viatcheslav A.; Fromm, Bastian

doi:10.1016/j.parint.2017.09.006

Cited by 10 publications

(10 citation statements)

References 21 publications

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“…squamata were shared with all other flatworms studied so far [39,48,56,57] . Interestingly, some of the gastrotrich miRNAs showed extended precursor lengths of up to 386 nucleotides, which, to this date, has only been described in flatworms [39,50,58] .…”

Section: Discussionmentioning

confidence: 74%

“…Interestingly, we found seven miRNAs of conserved families with particularly long hairpins (Let-7, Mir-750, Mir-22, Mir-31, Mir-1992, Mir-1) that exceeded commonly observed hairpin-sizes of 60-90 nucleotides (nt) with miRNA precursor lengths up to 386 nucleotides (Mir-750) (Figure 1b and 1c). Such long precursors are so far only described in flatworms [39,[48][49][50] .…”

Section: The Microrna Complement Of Lepidodermella Squamatamentioning

confidence: 99%

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Evolutionary implications of the microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)

Fromm

Tosar

Aguilera

et al. 2018

Preprint

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183/200)Gastrotrichs -hairy bellies -are microscopic free-living animals inhabiting marine and freshwater habitats. Based on morphological and early molecular analyses, gastrotrichs were placed close to nematodes, but recent phylogenomic analyses have suggested their close relationship to flatworms (Platyhelminthes) within Spiralia. Small non-coding RNA data on e.g. microRNAs (miRNAs) and PIWI-interacting RNAs (piRNA) may help to resolve this long-standing question. MiRNAs are short post-transcriptional gene regulators that together with piRNAs play key roles in development. In a 'multi-omics' approach we here used small-RNA sequencing, available transcriptome and genomic data to unravel the miRNA-and piRNA complements along with the RNAi protein machinery of Lepidodermella squamata (Gastrotricha, Chaetonotida). We identified 52 miRNA genes representing 35 highly conserved miRNA families specific to Eumetazoa, Bilateria, Protostomia, and Spiralia, respectively, with overall high similarities to platyhelminth miRNA complements. In addition, we found four large piRNA clusters that also resemble flatworm piRNAs but not those earlier described for nematodes. Congruently, transcriptomic annotation revealed that the Lepidodermella protein machinery is highly similar to flatworms, too. Taken together, miRNA, piRNA and protein data support a close relationship of gastrotrichs and flatworms.

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Section: Discussionmentioning

confidence: 74%

Section: The Microrna Complement Of Lepidodermella Squamatamentioning

confidence: 99%

Evolutionary implications of the microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)

Fromm

Tosar

Aguilera

et al. 2018

Preprint

Self Cite

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“…There was an almost complete set of the expected miRNA families and the spiralian-specific MIR-1992, but no Ecdysozoa- or Nematoda-specific miRNAs were detected. Some of the few losses observed in L. squamata were shared with all other flatworms studied so far [40,49,61,62]. Interestingly, some of the gastrotrich miRNAs showed extended precursor lengths of up to 386 nucleotides, which, to this date, has only been described in flatworms [40,51,63].…”

Section: Discussionmentioning

confidence: 89%

“…Interestingly, we found seven miRNAs of conserved families with particularly long hairpins (Let-7, Mir-750, Mir-22, Mir-31, Mir-1992, Mir-1) that exceeded commonly observed hairpin-sizes of 60–90 nucleotides (nt) with miRNA precursor lengths up to 386 nucleotides (Mir-750) (Figure 1b,c). Such long precursors are so far only described in flatworms [40,49,50,51].…”

Section: Resultsmentioning

confidence: 99%

Evolutionary Implications of the microRNA- and piRNA Complement of Lepidodermella squamata (Gastrotricha)

Fromm

Tosar

Aguilera

et al. 2019

ncRNA

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Gastrotrichs—'hairy bellies’—are microscopic free-living animals inhabiting marine and freshwater habitats. Based on morphological and early molecular analyses, gastrotrichs were placed close to nematodes, but recent phylogenomic analyses have suggested their close relationship to flatworms (Platyhelminthes) within Spiralia. Small non-coding RNA data on e.g., microRNAs (miRNAs) and PIWI-interacting RNAs (piRNA) may help to resolve this long-standing question. MiRNAs are short post-transcriptional gene regulators that together with piRNAs play key roles in development. In a ‘multi-omics’ approach we here used small-RNA sequencing, available transcriptome and genomic data to unravel the miRNA- and piRNA complements along with the RNAi (RNA interference) protein machinery of Lepidodermella squamata (Gastrotricha, Chaetonotida). We identified 52 miRNA genes representing 35 highly conserved miRNA families specific to Eumetazoa, Bilateria, Protostomia, and Spiralia, respectively, with overall high similarities to platyhelminth miRNA complements. In addition, we found four large piRNA clusters that also resemble flatworm piRNAs but not those earlier described for nematodes. Congruently, transcriptomic annotation revealed that the Lepidodermella protein machinery is highly similar to flatworms, too. Taken together, miRNA, piRNA, and protein data support a close relationship of gastrotrichs and flatworms.

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“…Among them, 27 miRNAs were conserved and 24 miRNAs were possibly species-specific [ 103 ]. However, reduced variation in the miRNAs of C. sinensis and high conservation have been reported [ 104 ]. Additionally, the genomic arrangement of some of these miRNAs has been determined.…”

Section: Mirnas Of Clonorchis Sinensismentioning

confidence: 99%

Roles of microRNAs and Long Non-Coding RNAs Encoded by Parasitic Helminths in Human Carcinogenesis

Leija-Montoya

González-Ramírez

Martínez-Coronilla

et al. 2022

IJMS

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Infectious agents such as viruses, bacteria, and parasites can lead to cancer development. Infection with the helminthic parasite Schistosoma haematobium can cause cancer of the urinary bladder in humans, and infection with the parasites Clonorchis sinensis and Opisthorchis viverrini can promote cholangiocarcinoma. These three pathogens have been categorized as “group 1: carcinogenic to humans” by the International Agency for Research on Cancer (IARC). Additionally, the parasite Schistosoma japonicum has been associated with liver and colorectal cancer and classified as “group 2B: possibly carcinogenic to humans”. These parasites express regulatory non-coding RNAs as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), which modulate genic expression in different biological processes. In this review, we discuss the potential roles of miRNAS and lncRNAs encoded by helminthic parasites that are classified by the IARC as carcinogenic and possibly carcinogenic to humans. The miRNAs of these parasites may be involved in carcinogenesis by modulating the biological functions of the pathogen and the host and by altering microenvironments prone to tumor growth. miRNAs were identified in different host fluids. Additionally, some miRNAs showed direct antitumoral effects. Together, these miRNAs show potential for use in future therapeutic and diagnostic applications. LncRNAs have been less studied in these parasites, and their biological effects in the parasite–host interaction are largely unknown.

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Extreme conservation of miRNA complements in opisthorchiids

Cited by 10 publications

References 21 publications

Evolutionary implications of the microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)

Evolutionary implications of the microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)

Evolutionary Implications of the microRNA- and piRNA Complement of Lepidodermella squamata (Gastrotricha)

Roles of microRNAs and Long Non-Coding RNAs Encoded by Parasitic Helminths in Human Carcinogenesis

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