Extrathymic T cell differentiation

Rocha, Bénédita; Guy‐Grand, Delphine; Vassalli, Pierre

doi:10.1016/0952-7915(95)80008-5

Cited by 123 publications

(99 citation statements)

References 53 publications

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“…It is discordant with some of the results obtained with adult mice, in which CD8␣␣ TCR␣␤ ϩ and TCR␥␦ ϩ lymphocytes are considered to be primarily of extrathymic origin (1,2). However, several studies suggest that a subset of both TCR␣␤ and TCR␥␦ murine CD8␣␣ iIELs is derived from the fetal/neonatal thymus (5)(6)(7)(8).…”

Section: Discussionmentioning

confidence: 40%

“…This unusual CD8␣␣ expression is also shared by an intestinal TCR ϩ , NK-like cell population (10 -12). The experiments suggesting an extrathymic origin for TCR ϩ cells were conducted in immunocompromised mice (1,2). However, in sheep, thymectomy in utero results in severe persistent depletion of peripheral T cells and a dramatic drop in the number of intraepithelial lymphocytes (IELs) 4 in the intestine of lambs during the first year of life (13).…”

mentioning

confidence: 99%

“…Although most peripheral T cells arise and expand in the thymus before they migrate to the periphery, it was proposed that the intestinal epithelium contains a thymus-independent T cell population that differentiates according to the epithelial microenvironment (1)(2)(3)(4). These cells express the coreceptor CD8␣ as a homodimer, whereas thymusderived T cells bear CD8␣␤ heterodimers as well as CD8␣␣ homodimers (5)(6)(7)(8)(9).…”

mentioning

confidence: 99%

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Intestinal CD8αα and CD8αβ Intraepithelial Lymphocytes Are Thymus Derived and Exhibit Subtle Differences in TCRβ Repertoires

Imhof

Dunon

Courtois

et al. 2000

The Journal of Immunology

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Intraepithelial lymphocytes (IEL) of the small intestine are anatomically positioned to be in the first line of cellular defense against enteric pathogens. Therefore, determining the origin of these cells has important implications for the mechanisms of T cell maturation and repertoire selection. Recent evidence suggests that murine CD8αα intestinal IELs (iIELs) can mature and undergo selection in the absence of a thymus. We analyzed IEL origin by cell transfer, using two congenic chicken strains. Embryonic day 14 and adult thymocytes did not contain any detectable CD8αα T cells. However, when TCR+ thymocytes were injected into congenic animals, they migrated to the gut and developed into CD8αα iIELs, while TCR− T cell progenitors did not. The TCR Vβ1 repertoire of CD8αα+ TCR Vβ1+ iIELs contained only part of the TCR Vβ1 repertoire of total iIELs, and it exhibited no new members compared with CD8+ T cells in the thymus. This indicated that these T cells emigrated from the thymus at an early stage in their developmental process. In conclusion, we show that while CD8αα iIELs originate in the thymus, T cells acquire the expression of CD8αα homodimers in the gut microenvironment.

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Section: Discussionmentioning

confidence: 40%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Intestinal CD8αα and CD8αβ Intraepithelial Lymphocytes Are Thymus Derived and Exhibit Subtle Differences in TCRβ Repertoires

Imhof

Dunon

Courtois

et al. 2000

The Journal of Immunology

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“…There is a distinct lineage of TCR␣␤ T cells that are thought to mature in the intestine, many of which are of CD8 ϩ CD4 Ϫ phenotype (23,24). In addition, cells of other lineages can express the CD8 molecule, such as NK cells (25), TCR␥␦ T cells (26), or dendritic cells (27).…”

Section: Majority Of Cd8␣ ϩ Cells In ␤ 2 M ϫ/ϫ Mice Do Not Express Cd8␤mentioning

confidence: 99%

“…Any of these cell types, or a combination of them, could potentially represent significant portion of the CD8 ϩ cell pool in ␤ 2 m Ϫ/Ϫ mice. A common denominator of all these cells is the form of the CD8 molecule they express: all of them express a CD8␣ homodimer (23,24), while thymus-derived TCR␣␤ cells express a heterodimer consisting of CD8␣-and CD8␤-chains. Thus CD8␤ expression could be used to infer the origin of the CD8 ϩ cells.…”

Section: Majority Of Cd8␣ ϩ Cells In ␤ 2 M ϫ/ϫ Mice Do Not Express Cd8␤mentioning

confidence: 99%

αβTCR+ Cells Are a Minimal Fraction of Peripheral CD8+ Pool in MHC Class I-Deficient Mice

Nešić

Santori

Vukmanović

2000

The Journal of Immunology

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MHC class I molecules play a role in the maintenance of the naive peripheral CD8+ T cell pool. The mechanisms of the peripheral maintenance and the life span of residual CD8+ cells present in the periphery of β2-microglobulin-deficient (β2m−/−) mice are unknown. We here show that very few CD8+ cells in β2m−/− mice coexpress CD8β, a marker of the thymus-derived CD8+ T cells. Most of the CD8α+ cells express CD11c and can be found in β2m/RAG-2 double-deficient mice, demonstrating that these cells do not require rearranged Ag receptors for differentiation and survival and may be of dendritic cell lineage. Rare CD8α+CD8β+ cells can be detected following in vivo alloantigenic stimulation 2 wk after the adult thymectomy. Selective MHC class I expression by bone marrow-derived cells does not lead to an accumulation of CD8β+ cells in β2m−/− mice. These findings demonstrate that 1) thymic export of CD8+ T cells in β2m−/− mice is reduced more severely than previously thought; 2) non-T cells expressing CD8α become prominent when CD8+ T cells are virtually absent; 3) at least some β2m−/− CD8+ T cells have a life span in the periphery comparable to wild-type CD8+ cells; and 4) similar ligands induce positive selection in the thymus and survival of CD8+ T cells in the periphery.

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Flow cytometry description of a novel CD3?/CD7+ intraepithelial lymphocyte subset in human duodenal biopsies: Potential diagnostic value in coeliac disease

Eirás¹,

Roldán²,

Camarero³

et al. 1998

Cytometry

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Extrathymic T cell differentiation

Cited by 123 publications

References 53 publications

Intestinal CD8αα and CD8αβ Intraepithelial Lymphocytes Are Thymus Derived and Exhibit Subtle Differences in TCRβ Repertoires

Intestinal CD8αα and CD8αβ Intraepithelial Lymphocytes Are Thymus Derived and Exhibit Subtle Differences in TCRβ Repertoires

αβTCR+ Cells Are a Minimal Fraction of Peripheral CD8+ Pool in MHC Class I-Deficient Mice

Flow cytometry description of a novel CD3?/CD7+ intraepithelial lymphocyte subset in human duodenal biopsies: Potential diagnostic value in coeliac disease

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