Extrahepatic Expression of NAD(P)H:Menadione Oxidoreductase, UDP Glucuronosyltransferase-1A6, Microsomal Aldehyde Dehydrogenase, and Hepatic Nuclear Factor-1α mRNAs inch/chand14CoS/14CoSMice

Vasiliou, Vasilis; Reuter, Stephen F.; Nebert, Daniel W.

doi:10.1006/bbrc.1997.6384

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…HNF-1␣ has been reported to be a member of the nuclear receptor superfamily that regulates the mouse and human UGT1A1 gene (Bernard et al, 1999), the mouse UGT1A6 gene (Vasiliou et al, 1997), the rat UGT1A7 gene (Metz et al, 2000), the rat UGT2B1 gene (Hansen et al, 1997), and the human UGT2B17 gene (Gregory et al, 2000). Since the reported 5Ј-flanking sequence of the UGT2B15 gene does not contain a putative HNF-1␣ binding site , it is reasonable that the amount of HNF-1␣ mRNA does not affect the dmd.aspetjournals.org expression levels of UGT2B15 mRNA.…”

Section: Resultsmentioning

confidence: 99%

Hepatocyte Nuclear Factor-1α Is a Causal Factor Responsible for Interindividual Differences in the Expression of UDP-Glucuronosyltransferase 2B7 mRNA in Human Livers

Toide

Takahashi²,

Yamazaki³

et al. 2002

Drug Metab Dispos

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This article is available online at http://dmd.aspetjournals.org ABSTRACT:UDP-glucuronosyltransferase (UGT) 2B7 is one of the most important UGT isozymes expressed in human livers. This enzyme is reported to show more than 10-fold interindividual differences in its enzyme activities. Thus, the amounts of UGT2B7 mRNA in 12 human livers were quantified by quantitative reverse transcriptionpolymerase chain reaction. The amounts of UGT2B7 mRNA in the subjects ranged from 0.22 to 2.63 copies/10 3 copies of ␤-actin. A novel point mutation (؊253G to A) found in this study did not affect the level of UGT2B7 mRNA in the subjects. To clarify a causal factor(s) determining the expression level of UGT2B7 mRNA, we examined the correlation between the amounts of mRNAs for UGT2B7 and hepatocyte nuclear factor (HNF)-1␣, which regulates the expression of UGT2B7 gene. HNF-1␣ mRNA was expressed at a level ranging from 2.99 to 24.76 copies/10 6 copies of ␤-actin in the subjects. The amounts of mRNAs for UGT2B7 expressed in these individual liver samples were highly associated with the amount of mRNA for HNF-1␣ (r ‫؍‬ 0.786, p ‫؍‬ 0.002), suggesting that HNF-1␣ is a factor limiting the expression of UGT2B7 mRNA and a causal factor responsible for an interindividual difference in human livers.

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Section: Resultsmentioning

confidence: 99%

Hepatocyte Nuclear Factor-1α Is a Causal Factor Responsible for Interindividual Differences in the Expression of UDP-Glucuronosyltransferase 2B7 mRNA in Human Livers

Toide

Takahashi²,

Yamazaki³

et al. 2002

Drug Metab Dispos

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“…Ceruloplasmin has been associated with anti-oxidant activity in patients with chronic prostatitis [109][110][111]; transferrin has been found elevated in the prostatic fluid from patient with prostate cancer [112][113][114]. Menadione oxidoreductase-1 and UGT1A6 are both phase II metabolizing enzymes and are regulated in a similar way by oxidative stress [115,116].…”

Section: Discussionmentioning

confidence: 99%

Aging-associated changes in gene expression in the ACI rat prostate: Implications for carcinogenesis

Reyes

Iatropoulos

et al. 2005

Prostate

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“…HNF-1α mRNA is present in new-born mouse liver (Vassiliou et al 1997) and adult rat liver (Frain et al 1989;Baumhueter et al 1990), kidney, small intestine and stomach. XGal staining in transgenic mice (Pontoglio et al 1996) detects HNF-1α expression in all the hepatocytes, in kidney proximal and distal convoluted tubules, and in many cells in the deep part of the gastric glands.…”

Section: Pcd/dcoh Immunoreactivity and Hnf-1mentioning

confidence: 99%

Immunohistochemical localisation of pterin-4α-carbinolamine dehydratase in rat peripheral organs

Résibois¹,

Cuvelier²,

Svoboda

et al. 1999

Histochemistry and Cell Biology

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The bifunctional protein PCD/DCoH is both a pterin-4alpha-carbinolamine dehydratase (PCD) involved in the recycling of tetrahydrobiopterin (BH4) and a dimerisation cofactor (DCoH) of the hepatic nuclear factor 1alpha (HNF-1alpha). An antiserum raised against rat PCD/DCoH was used to localise the protein in peripheral organs. In liver, all the hepatocytes but not the other cell types are immunoreactive. In kidney, the protein is prevalent in the proximal and distal convoluted tubules. In adrenals, all the cells of the medulla are labelled. Positive nerve cells occur in myenteric ganglia of the whole gastrointestinal tract and in the intestinal submucous ganglia. Many positive endocrine cells are present in the epithelium. The immunoreactivity is either cytoplasmic (hepatocytes, convoluted tubules of the kidney and part of the gastrointestinal endocrine cells) or prominently nuclear (kidney collecting tubules, adrenals, intestinal neural plexuses and part of the gastrointestinal endocrine cells). Our results show that PCD/DCoH is present in cells expressing enzymes that use BH4 as a cofactor and/or HNF-1alpha. In addition, PCD/DCoH is present in other cells, for example, neurons in the submucosal plexus. This fact and the prominent nuclear immunoreactivity found in all the positive cells derived from the neural crests argue in favour of a new, still unknown function for the protein.

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Extrahepatic Expression of NAD(P)H:Menadione Oxidoreductase, UDP Glucuronosyltransferase-1A6, Microsomal Aldehyde Dehydrogenase, and Hepatic Nuclear Factor-1α mRNAs inch/chand14CoS/14CoSMice

Cited by 4 publications

References 49 publications

Hepatocyte Nuclear Factor-1α Is a Causal Factor Responsible for Interindividual Differences in the Expression of UDP-Glucuronosyltransferase 2B7 mRNA in Human Livers

Hepatocyte Nuclear Factor-1α Is a Causal Factor Responsible for Interindividual Differences in the Expression of UDP-Glucuronosyltransferase 2B7 mRNA in Human Livers

Aging-associated changes in gene expression in the ACI rat prostate: Implications for carcinogenesis

Immunohistochemical localisation of pterin-4α-carbinolamine dehydratase in rat peripheral organs

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