Extracts of Terminalia sericea Enhance Cell Migratory Activity of Endothelial Hybrid and Fibroblast Cells In Vitro

Abstract

is a plant that has been used amongst others medicinally to treat wounds. The aim of this study was to assess the wound healing ability of Hot water, methanol, ethyl acetate, and hexane extracts were prepared. Thin layer chromatography (TLC) and ultra-performance liquid chromatography time of flight mass spectrometry (UPLC-TOF-MS) were used to determine the phytochemical classes and genus specific compounds present in the plant. Cytotoxicity was assessed in the SC-1 fibroblast and EA.hy926 endothelial hybrid cell lines using the sulforhodamine B assay. The effect of the extracts on cellular migration in both cell lines was assessed using the scratch assay. The major phytochemical classes detected in the extracts using TLC were alkaloids, coumarins, flavonoids, glycosides, phenolics, saponins, sterols, and terpenoids. The genus-specific compounds punicalagin, sericoside, anolignan B, and arjunic acid were identified in the extracts by means of UPLC-QTOF-MS. Cytotoxicity was not observed after 24 h of exposure and a generally low cytotoxic trend was noted after 72 h. A significant (p < 0.05) enhancement of cell migration in both cell lines was noted in the scratch assay. The wound healing ability of is mainly attributed to the migratory and proliferative activity of the extracts responsible for the acceleration of wound closure. Isolation and individualized testing of the active compounds is warranted.

An Inventory of South African Medicinal Plants Used in the Management of Sexually Transmitted and Related Opportunistic Infections: An Appraisal and Some Scientific Evidence (1990–2020)

“…In addition to exhibiting a potent enhanced cell migratory activity of endothelial hybrid and fibroblast cells in vitro, various extracts from Terminalia sericea were found to be not cytotoxic to a variety of cell lines, including SC-1 fibroblasts, the EA.Hy926 endothelial hybrid, and Vero cell lines [ 130 , 131 ]. Although clinical trials are of paramount importance in ethnopharmacology, it is important to note that South African research in that regard is lacking.…”

Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

“…Ultraperformance liquid chromatography and time‐of‐flight mass spectrometry (UPLC‐TOF‐MS) were conducted on a Waters instrument coupled in tandem to a Waters SYNAPT G1 HDMS mass spectrometer and used to generate accurate mass data. The chromatographic separation followed the procedure described by Parkar et al . MassLynx 4.1 (SCN 872) software was used to control the hyphenated system as well as for data manipulation.…”

“…[24] Ultraperformance liquid chromatography and time-of-flight mass spectrometry (UPLC-TOF-MS) were conducted on a Waters instrument coupled in tandem to a Waters SYNAPT G1 HDMS mass spectrometer and used to generate accurate mass data. The chromatographic separation followed the procedure described by Parkar et al [25] MassLynx 4.1 (SCN 872) software was used to control the hyphenated system as well as for data manipulation. Cell culture and maintenance C2C12 myoblasts were purchased from the American Tissue Culture Collection (ATCC, CRL-1772) and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated foetal calf serum (FCS), 100 lg/ml streptomycin and 100 mg/l penicillin at 37°C in a humidified incubator containing 5% CO 2 .…”

Assessment of Wound Healing Potentials of Dichloromethane Extract of Heterotis rotundifolia Leaves in Female Wistar Rats

“…Wounds may be created by physical, chemical, thermal, microbial, or immunological damage to the tissues [1,2,3]. Wound healing is the integration of a complex cascade of biological processes occurring in a sequential overlapping manner characterized by four phases; homeostasis, inflammation, proliferation and remodelling towards the regeneration and repair of lost tissue [4,5]. Homeostasis begins when the injury occurs in skin or tissues leading to the damage of blood vessels.…”

“…The damaged blood vessels and adjacent arteries initiate the vasoconstriction process. Homeostasis usually lasts for few hours [5].Inflammatory phase begins the healing process after bleeding has been controlled and usually occurs from hours to days. It is depicted by rubor (redness), calor (warmth), tumor (swelling), dolor (pain), and functiolaesa (loss of function) [4].…”