Extracts of medical plants suppress the SOS response and reduce mutagenesis in E. coli

Abstract

One of the promising directions in the fight against the emergence and spread of farm animal microbiota resistance factors is the development and search for feed additives that can inhibit the SOSresponse. SOS-response is one of the main mechanisms of the occurrence of mutations in bacteria. Plants used in traditional medicine can be a promising source of safe substances that reduce the SOS-response of bacteria. A screening of plants potentially containing substances with antiSOS activity was performed. During the initial screening, the E. coli MG 1655 pRecA-lux biosensor strain with ciprofloxacin as RecA inducer was used. Seven plants were identified whose extracts reduced the expression of the RecA operon. In further experiments on bacteria exposed to antibiotics, we identified four plants whose exstracts significantly reduced the mutagenesis rate of clinical E. coli strains: Austrian broom (Cytisus austriacus), greater celandine (Chelidonium majus), walnut (Juglans regia) and smooth sumac (Rhus glabra).

The volume of the sample as a factor of survival of sturgeon spermatozoa after cryopreservation

“…This allows us to open new areas of research in the field of cryopreservation of fish reproductive cells and ensure the improvement of previously known technologies. [18][19] When using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets, the results were obtained (Figure 3, 4). When applying the seminal fluid to the net frame, a thin film was formed.…”

Innovative technologies and equipment from “Amazone” company for fertilizer application

“…Although developed and serially produced for the agroindustrial complex of Russia, special equipment and equipment for it for the introduction of liquid fertilizers ensures the effective use of liquid fertilizers according to various technologies, taking into account the biological characteristics of agricultural cultivation, the type of fertilizers, advanced methods of application both superficially and locally-subsoil. [15][16][17][18] In this connection, systematizing the machines and equipment for the introduction of liquid fertilizers, the following technologies can be distinguished ( Fig. 4): Iwith sprayers with multi-jet nozzles: 1onto the soil surface; 2onto the plant from plants and on the soil surface; IIwith sprayers with extension hoses to the soil surface; IIIwith sprayers with extension hoses to the soil surface and plants; IVwith universal unit FDC (JSC "Eurotechnika") for the working bodies of the cultivator Cenius during the main and pre-sowing soil cultivation; Vwith universal unit FDC when sowing grain crops under the anchor openers of the Primera DMC seeder and chisel openers of the Condor seeder, as well as when sowing row crops under the disc coulters of the precision seeder -EDX (all machines are produced in Russia -Samara, JSC "Eurotechnika").…”

Comprehensive assessment of aquaculture potential of the southern and south-eastern waterbodies in the Rostov region

“…Group 4: not recommended for fish farming (less than 4.2 points). Note: in brackets the point coefficient is indicated in different seasons of the year -spring, summer and autumn [16][17].…”