Extracts of Leaves of Six Locally Available Plants from Bagmati Province of Nepal as Potent Inhibitors of Alpha-amylase, Lipase, Tyrosinase, Elastase, and Cholinesterases

Abstract

Background: Medicinal plants and their products are gaining global popularity due to its several health benefits. However, the biological activities of the vast majority of medicinal plant species have not been explored yet. In this study, we evaluated the enzyme inhibitory potential six medicinal plant species involved in digestion, skin-related problems, and neurological problems. Methods: The 80% methanol extract of six locally available plants from the Bagmati province of Nepal were analyzed for their flavonoids content, phenolics content, antioxidant activity, and enzymes inhibitory potential. Antioxidant activity was analyzed by the DPPH assay. Alpha-amylase inhibition was carried out by the DNSA method. Lipase, tyrosinase, elastase, acetylcholinesterase, and butyrylcholinesterase inhibition were analyzed by using p-NPB, L-DOPA, AAAPVN, acetylthiocholine, and butyrylthiocholine as a respective substrate. Results: Among the analyzed plants species, Artocarpus heterophyllus possess highest α-amylase (IC50=6.28±0.01 μg/mL), lipase (IC50=475.14±3.17 μg/mL), elastase (IC50=72.75±3.41 μg/mL) and acetylcholinesterase (IC50= 68.66±1.71 μg/mL) inhibition, whereas, Actinidia deliciosa possess highest tyrosinase inhibition (IC50=139.87±0.72 μg/mL) and butyrylcholinesterase inhibition (IC50=18.32±0.44 μg/mL). Furthermore, Jasminum humile showed no inhibitory tendencies against elastase and Lygodium japonicum showed no inhibition towards lipase and elastase at given concentration range. Conclusions: Our study revealed that A. deliciosa and A. heterophyllus are the potential source of α-amylase, lipase, elastase, acetylcholinesterase, and butyrylcholinesterase inhibitors. Based on our finding, we concluded that the analyzed plant species are of great scientific interest to the pharmaceutical, cosmetics, and food industry.

Non-Thermal Plasma: A Promising Technology for the Germination Enhancement of Radish (Raphanus sativus) and Carrot (Daucus carota sativus L.)

“…Scavenging, Chlorophyll, and Carotenoid Contents. Te estimation of favonoids, phenolics, scavenging, and chlorophyll contents were done using standard protocols as done by various researchers [56][57][58].…”

“…Antioxidants provide electrons to injured cell, preventing, and stabilizing free radical damage. Radical scavenging is crucial in diseases prevention [57]. Plant extracts are often tested for their antioxidant activities using the DPPH technique.…”

Impact of non-thermal plasma treatment on the seed germination and seedling development of carrot (Daucus carota sativus L.)

“…The ability of antioxidants to donate hydrogen is assumed to be the reason for their action on DPPH. Free radical scavenging actions are critical for preventing the harmful effects of free radicals in various diseases [62]. The DPPH free radical scavenging method is widely used to test the antioxidant properties of plant extracts.…”