Extracts of Hesperozygis ringens (Benth.) Epling: in vitro and in vivo antibacterial activity against fish pathogenic bacteria

Abstract

Aims This study investigated the in vitro antibacterial activity of Hesperozygis ringens (Benth.) Epling leaf extracts against fish pathogenic bacteria, as well as the in vivo activity of the most active extract in silver catfish (Rhamdia quelen) experimentally infected with Aeromonas hydrophila. Moreover, the chemical composition of the extract used in the survival assay was evaluated. Methods and Results Only hexane extract (HEHR) showed in vitro antibacterial activity (MIC and MBC ranging from 1600 to 3200 μg ml−1) against clinical isolates of A. hydrophila, Raoultella ornithinolytica and Citrobacter freundii, obtained from naturally infected silver catfish, and A. hydrophilaATCC 7966. The major compound of the volatile fraction of HEHR was determined as pulegone. HEHR promoted a 93·33% relative survival rate of silver catfish experimentally infected with A. hydrophila 7 days after a single therapeutic bath at 30 mg l−1, while florfenicol at 4 mg l−1, which promoted a 60% relative survival rate. Conclusions The antibacterial activity of H. ringens (Benth.) Epling leaf extracts seems to be related to phytochemicals of apolar character, since HEHR promoted better survival rate of infected animals than florfenicol. Significance and Impact of the Study The HEHR has potential to be used in the control and treatment of bacterial infections in organic aquaculture.

Mitigating Aeromonas hydrophila infection in Nile tilapia through dietary Basella alba and Withania somnifera supplementation: A bioassay‐guided fractionation approach

“…Antibacterial activity of plant material may be contingent on the presence of extractable active compounds, which is dependent on the property and polarity of extracting solvent (Onyebuchi & Kavaz, 2020). Variable antimicrobial efficacy against A. hydrophila was earlier observed for different solvent extracts of Hesperozygis ringens (Rosa et al, 2019). In the pres- extracts rich in phenolic compounds were postulated to destroy bacterial cell membranes and show antimicrobial activity (Monir et al, 2020).…”

ANÁLISE CIENCIOMÉTRICA DAS PESQUISAS CIENTÍFICAS SOBRE MICROPROPAGAÇÃO IN VITRO DE Cattleya walkeriana DOS ANOS DE 1999 A 2019

“…Most reports in the literature on H. ringens involve the characterization of essential oil (Pinheiro et al 2016;Rosa et al 2019). Thus, in order to expand the potential of the plant, in previous studies we investigated ultrasonic assisted extraction with ethanol as solvent (UAE-EtOH), detecting rosmarinic acid, chlorogenic acid, caffeic acid, ferulic acid, rutin, ρ-coumaric acid, vanillic acid, resveratrol, luteolin, quercetin, apigenin and kaempferol.…”

Flora Do Amapá: Vitaceae Juss

“…Most reports in the literature on H. ringens involve the characterization of essential oil (Pinheiro et al 2016;Rosa et al 2019). Thus, in order to expand the potential of the plant, in previous studies we investigated ultrasonic assisted extraction with ethanol as solvent (UAE-EtOH), detecting rosmarinic acid, chlorogenic acid, caffeic acid, ferulic acid, rutin, ρ-coumaric acid, vanillic acid, resveratrol, luteolin, quercetin, apigenin and kaempferol.…”