Extracts from Lupinus albescens: antioxidant power and antifungal activity in vitro against phytopathogenic fungi

Abstract

Fungi are considered the most damaging microorganisms in agriculture. The indiscriminate use of chemical treatments in agricultural products causes the development of pest resistance and affects human health. An alternative to synthetic fungicides is the use of natural products such as plant extracts for the management of fungal diseases in plants. Extracts from different parts of Lupinus albescens (roots, stalks, leaves, and flowers) were obtained by extraction using supercritical carbon dioxide (CO) or compressed liquefied petroleum gas (LPG). Thereafter, the antioxidant activity of each extract was measured, and the antifungal activity in vitro of extracts was evaluated against Fusarium oxysporum and Fusarium verticillioides. For a concentration of 5000 mg/L, the half maximal inhibitory concentration (IC) ranged from 29.25 μg/mL to 192.96 μg/mL. Antifungal tests showed that all matrices presented inhibitory effect against both fungi tested. The extracts obtained from roots by CO and LPG presented 70.1% and 65.1% inhibition against F. oxysporum, and 67.8% and 61.2% inhibition against F. verticillioides, respectively. These results suggest that the extracts obtained from L. albescens by extractions using supercritical CO and compressed LPG might be a potential source of antioxidants and natural fungicides.

Importance of Lupinus albescens in agricultural and food-related areas: A review

“…All matrices presented a high inhibition power for both fungi: 70.1% inhibition against F. oxysporum and 67.8% inhibition against F. verticillioides. The root matrix was highlighted, because its extracts presented the best results for both tests (antifungal and antioxidant activities) (Confortin et al 2018). The authors infer that their outcomes were promising in terms of antioxidant activity.…”

Plant extracts in agriculture and their applications in the treatment of seeds

“…In recent years, one of the main demands of the population and a challenge faced by the agricultural sector is the need for safer and more sustainable approaches regarding the management of agricultural production systems in view of the harmful effects generated by the high toxicity and low biodegradability of diverse synthetic agrochemicals used (BoITEUX et al, 2019). An increase in the visibility of researches using plant extracts in agriculture is then perceived, demonstrating potential alternatives for application in production systems (KHAN et al, 2020;CoNFoRTIN et al, 2019).…”

Quinolizidine-Based Variations and Antifungal Activity of Eight Lupinus Species Grown under Greenhouse Conditions

“…In addition, QA-rich Lupinus extracts (QREs) have shown antimicrobial activity against different microorganisms. For instance, the extracts of L. mutabilis seeds have inhibited 95% of the growth of Staphylococcus aureus [ 18 ], while Candida fungi have shown >60% inhibition in the presence of QREs of L. arboreus leaves [ 19 ], L. albescens [ 20 ], L. angustifolius [ 21 ], and L. densiflorus [ 22 ]. The seed-derived QREs from L. exaltatus and L. mexicanus have also been reported, showing inhibition >72% against Sclerotium rolfsii , Alternaria solani , Rhizoctonia solani , and F. oxysporum [ 23 ].…”

“…The seed-derived QREs from L. exaltatus and L. mexicanus have also been reported, showing inhibition >72% against Sclerotium rolfsii , Alternaria solani , Rhizoctonia solani , and F. oxysporum [ 23 ]. In the case of phytopathogens of the genus Fusarium, F. oxysporum and F. verticillioides showed susceptibility to QREs of L. albescens , with IC 50 values < 30 μg/mL [ 20 ].…”