Extracts from Curcuma zedoaria Inhibit Proliferation of Human Breast Cancer Cell MDA‐MB‐231 In Vitro

Abstract

Objective. To evaluate the effect of petroleum ether extracts of Curcuma zedoaria on the proliferation of human triple negative breast cancer cell line MDA-MB-231. Methods. The reagents were isolated from Curcuma zedoaria by petroleum ether fraction. It was assayed by CCK8 for MDA-MB-231 cellular viability with various concentrations and days, cell cycle analyses, Western Blot analysis, and Realtime Reverse Transcriptase PCR analyses for chemokines molecules including E-cadherin, and E-selectin, and adhesion molecules including CCR7, SLC, SDF-1, and CXCR4. Epirubicin was used as control in the study. Results. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria (P < 0.05), and the inhibition rate was dependent on concentrations and time. Petroleum ether extracts of Curcuma zedoaria as well as Epirubicin produce a significant G0/G1 cell cycle arrest. The level of expression of proteins E-cadherin and E-cadherin mRNA was significantly increased, while proteins SDF-1, CCR7, and CXCR4 mRNA were decreased after being incubated with petroleum ether extracts of Curcuma zedoaria at the concentrations of 300 μg/mL than control (P < 0.05). The differences were that the protein CXCR4 mRNA expression level was higher than vehicle. Conclusions. MDA-MB-231 cells were inhibited by petroleum ether extracts of Curcuma zedoaria.

Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma

“…C. rhizoma is widely used in the therapy of UL but absolutely prohibited for the pregnant women in clinics (15–17). It possesses anti-inflammatory and anti-tumor activities and responds to multiple regulation of signaling pathways for ECM deposition (18), cell cycle arrest (19), and UL process (20). Moreover, TCM has long established the concise and synergistic effects of C. rhizoma and S. rhizoma , and, as a consequence, a compound with both was developed for UL therapies (21).…”

Network Pharmacology-Based Validation of Caveolin-1 as a Key Mediator of Ai Du Qing Inhibition of Drug Resistance in Breast Cancer

“…Bunge (Chinese name Huang Qi, HQ), and Shi (courier) herb Glycyrrhiza uralensis Fisch (Chinese name Gan Cao, GC). Each herb in the formula can inhibit cancer growth via cell cycle arrest, apoptosis induction, and immune regulation ( Fu et al, 2014a ; Gao et al, 2014 ; Wang et al, 2014 , 2015b ; Feng et al, 2017 ). In addition, each herb is a drug that is frequently prescribed to cancer patients according to statistical analyses of the medication rules of national TCM masters ( Song et al, 2015 ).…”

Yanghe Huayan decoction inhibits the capability of trans-endothelium and angiogenesis of HER2+ breast cancer via pAkt signaling

“…Extract of Bai jie zi—sinapine exhibited growth-suppressive activity and reversed multidrug resistance in MCF/dox cancer cells [9]. Whereas E zhu extracts presented cytotoxic effect on BC cells and induced ROS formation and cell apoptosis [10,11]. The current study demonstrated that YHD exhibited antitumor activity against BC xenografts in nude mice.…”