Extractive Fermentation of Xylanase from Aspergillus tamarii URM 4634 in a Bioreactor

Silva, Anna Carolina da; Queiroz, Alana Emília Soares de França; Nascimento, Talita Camila Evaristo da Silva; Rodrigues, Cristine; Gomes, José Erick Galindo; Souza-Motta, Cristina Maria de; Vandenberghe, Luciana Porto de Souza; Medeiros, Érika Valente de; Moreira, Keila Aparecida; Herculano, Polyanna Nunes

doi:10.1007/s12010-014-0953-8

Cited by 7 publications

(7 citation statements)

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“…Typically, this species is associated with plant biomass degradation as a spoilage fungus, with reports of isolation from food and feed products (Rodrigues et al, 2011 ; Midorikawa et al, 2014 ; Martins et al, 2017 ; Prencipe et al, 2018 ). Previous studies have also highlighted the potential in this species in depolymerization of xylan, through secretion of β-1,4-endoxylanases (Gouda and Abdel-Naby, 2002 ; da Silva et al, 2014 ). Efficient secretion of such xylanases has also been reported in the strain A. tamarii BLU37 (Duarte et al, 2012 ; Monclaro et al, 2016 ).…”

Section: Discussionmentioning

confidence: 83%

Analysis of the Transcriptome in Aspergillus tamarii During Enzymatic Degradation of Sugarcane Bagasse

Midorikawa

Corrêa

Noronha

et al. 2018

Front. Bioeng. Biotechnol.

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The production of bioethanol from non-food agricultural residues represents an alternative energy source to fossil fuels for incorporation into the world's economy. Within the context of bioconversion of plant biomass into renewable energy using improved enzymatic cocktails, Illumina RNA-seq transcriptome profiling was conducted on a strain of Aspergillus tamarii, efficient in biomass polysaccharide degradation, in order to identify genes encoding proteins involved in plant biomass saccharification. Enzyme production and gene expression was compared following growth in liquid and semi-solid culture with steam-exploded sugarcane bagasse (SB) (1% w/v) and glucose (1% w/v) employed as contrasting sole carbon sources. Enzyme production following growth in liquid minimum medium supplemented with SB resulted in 0.626 and 0.711 UI.mL−1 xylanases after 24 and 48 h incubation, respectively. Transcriptome profiling revealed expression of over 7120 genes, with groups of genes modulated according to solid or semi-solid culture, as well as according to carbon source. Gene ontology analysis of genes expressed following SB hydrolysis revealed enrichment in xyloglucan metabolic process and xylan, pectin and glucan catabolic process, indicating up-regulation of genes involved in xylanase secretion. According to carbohydrate-active enzyme (CAZy) classification, 209 CAZyme-encoding genes were identified with significant differential expression on liquid or semi-solid SB, in comparison to equivalent growth on glucose as carbon source. Up-regulated CAZyme-encoding genes related to cellulases (CelA, CelB, CelC, CelD) and hemicellulases (XynG1, XynG2, XynF1, XylA, AxeA, arabinofuranosidase) showed up to a 10-fold log2FoldChange in expression levels. Five genes from the AA9 (GH61) family, related to lytic polysaccharide monooxygenase (LPMO), were also identified with significant expression up-regulation. The transcription factor gene XlnR, involved in induction of hemicellulases, showed up-regulation on liquid and semi-solid SB culture. Similarly, the gene ClrA, responsible for regulation of cellulases, showed increased expression on liquid SB culture. Over 150 potential transporter genes were also identified with increased expression on liquid and semi-solid SB culture. This first comprehensive analysis of the transcriptome of A. tamarii contributes to our understanding of genes and regulatory systems involved in cellulose and hemicellulose degradation in this fungus, offering potential for application in improved enzymatic cocktail development for plant biomass degradation in biorefinery applications.

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Section: Discussionmentioning

confidence: 83%

Analysis of the Transcriptome in Aspergillus tamarii During Enzymatic Degradation of Sugarcane Bagasse

Midorikawa

Corrêa

Noronha

et al. 2018

Front. Bioeng. Biotechnol.

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“…These systems are traditionally used for protein separation and can be applied to the separation of other biological products, such as low molecular weight compounds, organelles, and other macromolecules [97]. In the case of extractive fermentation, ATPS have been applied as extractive fermentation systems for different molecules, such as alkaline phosphatase [98], clavulanic acid [99], lipase [21], and xylanase [100].…”

Section: Techniques Used In Isprmentioning

confidence: 99%

“…On the other hand, lipase production did not follow this profile. Other studies have shown a negative effect of the PEG concentration on enzyme production [10,100,101].…”

Section: Techniques Used In Isprmentioning

confidence: 99%

In situ product recovery techniques aiming to obtain biotechnological products: A glance to current knowledge

Santos

Albuquerque

Ribeiro

et al. 2020

Biotech and App Biochem

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Biotechnology and bioengineering techniques have been widely used in the production of biofuels, chemicals, pharmaceuticals, and food additives, being considered a “green” form of production because they use renewable and nonpolluting energy sources. On the other hand, in the traditional processes of production, the target product obtained by biotechnological routes must undergo several stages of purification, which makes these processes more expensive. In the past few years, some works have focused on processes that integrate fermentation to the recovery and purification steps necessary to obtain the final product required. This type of process is called in situ product recovery or extractive fermentation. However, there are some differences in the concepts of the techniques used in these bioprocesses. In this way, this review sought to compile relevant content on considerations and procedures that are being used in this field, such as evaporation, liquid–liquid extraction, permeation, and adsorption techniques. Also, the objective of this review was to approach the different configurations in the recent literature of the processes employed and the main bioproducts obtained, which can be used in the food, pharmaceutical, chemical, and/or fuel additives industry. We intended to elucidate concepts of these techniques, considered very recent, but which emerge as a promising alternative for the integration of bioprocesses.

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“…Extractive fermentation in ATPS, a schematic setup of which is illustrated in Figure , is a process that integrates an extraction step in the first processing stage to allow for simultaneous product synthesis and removal. This can ensure the primary recovery and increase productivity by minimizing final product inhibition during fermentation . As suggested by the name, the microbial system is cultured in one of the two phases, while the target biomolecule is excreted and partitioned in the other phase.…”

Section: Introductionmentioning

confidence: 99%

“…This can ensure the primary recovery and increase productivity by minimizing final product inhibition during fermentation. 17 As suggested by the name, the microbial system is cultured in one of the two phases, while the target biomolecule is excreted and partitioned in the other phase. Such a system enables quick recovery of product without the need of harvesting biomass or cell disruption.…”

Section: Introductionmentioning

confidence: 99%

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

Marques

Santos-Ebinuma

Pessoa

et al. 2016

Biotechnology Progress

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In this work, the effects of agitation and aeration rates on aqueous two-phase system (ATPS)-based extractive fermentation of clavulanic acid (CA) by Streptomyces variabilis DAUFPE 3060 were investigated through a 2 full factorial design, where oxygen transfer rate (OTR) and oxygen uptake rate (OUR) were selected as the responses. Aeration rates significantly influenced cell growth, OUR, and CA yield, while OTR was practically the same in all the runs. Under the intermediate agitation (950 rpm) and aeration conditions (3.5 vvm) of the central point runs, it was achieved OTR of 1.617 ± 0.049 mmol L h , OUR of 0.132 ± 0.030 mmol L h , maximum CA production of 434 ± 4 mg L , oxygen mass transfer coefficient of 33.40 ± 2.01 s , partition coefficient of 66.5 ± 1.5, CA yield in the top and bottom phases of 75% ± 2% and 19% ± 1%, respectively, mass balance of 95% ± 4% and purification factor of 3.8 ± 0.1. These results not only confirmed the paramount role of O supply, broth composition and operational conditions in CA ATPS-extractive fermentation, but also demonstrated the possibility of effectively using this technology as a cheap tool to simultaneously produce and recover CA. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1444-1452, 2016.

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Extractive Fermentation of Xylanase from Aspergillus tamarii URM 4634 in a Bioreactor

Cited by 7 publications

References 50 publications

Analysis of the Transcriptome in Aspergillus tamarii During Enzymatic Degradation of Sugarcane Bagasse

Analysis of the Transcriptome in Aspergillus tamarii During Enzymatic Degradation of Sugarcane Bagasse

In situ product recovery techniques aiming to obtain biotechnological products: A glance to current knowledge

Effect of aeration and agitation on extractive fermentation of clavulanic acid by using aqueous two‐phase system

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