Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5′-hydroxypiroxicam in human plasma and urine

Avgerinos, A.; Axarlis, S.; Dragatsis, J.; Karidas, Th.; Malamataris, S.

doi:10.1016/0378-4347(95)00248-h

Cited by 31 publications

(11 citation statements)

References 13 publications

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“…Since the transdermal delivery avoids the gastrointestinal side effect and first-pass effect, many studies have been carried out in order to develop the percutaneous preparations of NSAIDs, including piroxicam and tenoxicam [1][2][3][4][5][6] number of high performance liquid chromatography (HPLC) methods with UV detection [7][8][9][10][11][12][13][14][15][16], amperometric detection [17] and tandem mass spectrometry (LC-MS/MS) [16,[18][19][20] were reported for the determination of piroxicam, meloxicam or tenoxicam in biological fluids; however, most of those methods presented insufficient sensitivity (limit of detection; 0.72-50 ng/ml), the use of large biological fluid volumes (0.25-1 ml plasma or urine) or chromatographic interferences. There was no LC-MS/MS method reported for the simultaneous determination of meloxicam, piroxicam and tenoxicam in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of piroxicam, meloxicam and tenoxicam in human plasma by liquid chromatography with tandem mass spectrometry

Lee

Kim

et al. 2005

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of piroxicam, meloxicam and tenoxicam in human plasma by liquid chromatography with tandem mass spectrometry

Lee

Kim

et al. 2005

Journal of Chromatography B

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“…1,9,11,12 Briefly, a twelve point scale was created using values from 0.05 to 20.0 mg/mL (piroxicam) and 0.05 to 10.0 mg/mL (5'-hydroxypiroxicam). 9 These predetermined concentrations of piroxicam and 5'-hydroxypiroxicam were aliquoted in methanol, dried, and then resuspended in 100 µL of the mobile phase (0.1 M phosphate buffer pH 3.2 and acetonitrile, 7:3 ratio) for analysis in HPLC injection flow 1 mL/min, 24°C with UV light (330 nm). All samples were analyzed during the same period and automatically injected using an injector (automatic injector SIL model, 20 AC HT, Shimadzu Corp., Kyoto, Japan).…”

Section: Quantification Of Piroxicam and 5'-hydroxypiroxicammentioning

confidence: 99%

“…1,9,11,12 Furthermore, naproxen was used as an internal standard. 9,12 Briefly, volunteers consumed a single dose of piroxicam (20 mg). The blood collection protocol consisted of blood samples taken before and after ingestion of this single 20 mg dose of piroxicam -immediately, 1, 2, 3, 4, 5, 6, 8, 11, 24, 48 and 72 h after intake.…”

Section: Patients and Administrationmentioning

confidence: 99%

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Effective method for the detection of piroxicam in human plasma using HPLC

et al. 2016

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Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used by the general population to alleviate inflammation and pain after oral surgeries. Piroxicam is among the most commonly used NSAIDs and excels in controlling pain, swelling, trismus and other common symptoms of inflammation. This study aimed to evaluate different concentrations of piroxicam and its major metabolite, 5'-hydroxypiroxicam, in human plasma samples over time using high performance liquid chromatography (HPLC) after liquid-liquid extraction. Briefly, 10 volunteers participated in this study after approval by the Ethics Committee of Bauru School of Dentistry, Universidade de São Paulo -USP, Brazil. Volunteers received a single dose oral of piroxicam (20 mg) and had blood collected at various times following an established protocol. The methodology of liquid-liquid extraction was effective for determining concentrations of piroxicam in plasma using HPLC in 10 out of 10 volunteers while 5'-hydroxypiroxicam was only detected in 2 out of 10 volunteers.

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“…18,19 Piroxicam (PX), 4-hydroxy-2-methyl-N-(2-pyridyl)-2H-1,2-benzothiazine-3-carboamide-1,1-dioxide (Scheme 1), is a nonsteroidal anti-inflammatory agent which is widely used in the treatment of rheumatic diseases. 20 The literature reports several analytical methods for the determination of PX in pharmaceutical samples and biological fluids [21][22][23][24][25][26] including spectrofluorimetry methods due to high sensitivity and selectivity. [27][28][29][30] In pharmaceutical preparations, PX is frequently combined with some vitamins of the B complex.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

2006

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One of the most serious problems that can occur in classical quantitative analysis is the presence of one or more spectral interferent-chemical species which affect the instrument response and which are unaccounted for in the calibration process. Advances in chemometric methods allow quantitative analysis in the presence of unidentified interferents if a threeway experimental data matrix is available for each sample. This property is the so-called "second-order advantage" 1 and it is based on the earlier work done in the psychometrics field. 2,3 The use of this advantage in analytical chemistry was proposed by Ho et al. 4 (rank annihilation factor analysis, RAFA), for the multicomponent analysis of fluorescent mixtures using excitation-emission matrix (EEM). Later, Lorber 5 and Sanchez and Kowalski 6 proposed new and simple solutions for the method of Ho et al. and now it is called the generalized rank annihilation method (GRAM).At present, analytical chemistry laboratories have instrumentation that easily generates multidimensional data structures of experimental data for each sample. Fluorescence is a particularly interesting technique because it allows, in a very straightforward manner, the acquisition of this type of information, of which the most typical example is the excitation-emission matrix (EEM). Several algorithms that use second-order data are described in the literature. [7][8][9] Of these, the generalized rank annihilation method (GRAM) 6,11,12 has interesting properties since it only requires two data matrices for quantification: one from the calibration sample and the other from the unknown sample. Recently, it has been proved that GRAM predicts results that are similar to those from the parallel factor analysis (PARAFAC) when only two samples are used, 9 so both methods can be useful as calibration methods for second-order data. But, unlike PARAFAC, GRAM is a non-iterative method which tries to implement the second-order advantage. Second-order calibration using GRAM has several distinct advantages. 13The most pronounced advantage is that it makes possible the quantification of the analyte(s) of interest in samples containing interferents. Those interferents can be completely unknown, that is, it is not necessary that they have been incorporated in the standards used for calibration. Another advantage is that it is possible to recover the individual instrument responses of the analyte(s) of interest, e.g., the excitation and emission spectra can be obtained for the individual analyte(s). This makes it possible to perform qualitative analysis and to check the identity of the analytes. The Kowalski group has proposed the trilinear decomposition (TLD) method. 14,15 In this work we focused our attention on the ability of GRAM for analyzing the second-order data.Fluorescence spectroscopy is a versatile tool mainly used because of its selectivity and sensitivity. Fluorescence spectra can be recorded in difference modes such as emission, excitation, synchronous and excitation-emission matrix (EEM). T...

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Extractionless high-performance liquid chromatographic method for the simultaneous determination of piroxicam and 5′-hydroxypiroxicam in human plasma and urine

Cited by 31 publications

References 13 publications

Simultaneous determination of piroxicam, meloxicam and tenoxicam in human plasma by liquid chromatography with tandem mass spectrometry

Simultaneous determination of piroxicam, meloxicam and tenoxicam in human plasma by liquid chromatography with tandem mass spectrometry

Effective method for the detection of piroxicam in human plasma using HPLC

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

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