Extraction, purification and characterization of phenazine from Pseudomonas aeruginosa isolate of wastewater sources: a panacea towards clinical pathogens

Thacharodi, Aswin; Priyadharshini, R.; Karthikeyan, G.; Jeganathan, C.; Reghu, Akhila P.; Pugazhendhi, Arivalagan

doi:10.1007/s13204-021-01944-y

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…Again, for the type strains of these three species the production of DAPG was experimentally validated [ 60 ]. Likewise, it has been reported that strains belonging to the species P. aeruginosa [ 61, 62 ], P. entomophila [ 63 ] and P. syringae [ 64 ] are able to produce phenazines, entolysin and syringolin A, respectively, something that agrees with the results of this study, supporting our predictions.…”

Section: Discussionsupporting

confidence: 93%

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Unveiling the genomic potential of Pseudomonas type strains for discovering new natural products

Saati‐Santamaría

Sélem-Mójica²,

Peral-Aranega

et al. 2022

Microbial Genomics

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Microbes host a huge variety of biosynthetic gene clusters that produce an immeasurable array of secondary metabolites with many different biological activities such as antimicrobial, anticarcinogenic and antiviral. Despite the complex task of isolating and characterizing novel natural products, microbial genomic strategies can be useful for carrying out these types of studies. However, although genomic-based research on secondary metabolism is on the increase, there is still a lack of reports focusing specifically on the genus Pseudomonas . In this work, we aimed (i) to unveil the main biosynthetic systems related to secondary metabolism in Pseudomonas type strains, (ii) to study the evolutionary processes that drive the diversification of their coding regions and (iii) to select Pseudomonas strains showing promising results in the search for useful natural products. We performed a comparative genomic study on 194 Pseudomonas species, paying special attention to the evolution and distribution of different classes of biosynthetic gene clusters and the coding features of antimicrobial peptides. Using EvoMining, a bioinformatic approach for studying evolutionary processes related to secondary metabolism, we sought to decipher the protein expansion of enzymes related to the lipid metabolism, which may have evolved toward the biosynthesis of novel secondary metabolites in Pseudomonas . The types of metabolites encoded in Pseudomonas type strains were predominantly non-ribosomal peptide synthetases, bacteriocins, N-acetylglutaminylglutamine amides and ß-lactones. Also, the evolution of genes related to secondary metabolites was found to coincide with Pseudomonas species diversification. Interestingly, only a few Pseudomonas species encode polyketide synthases, which are related to the lipid metabolism broadly distributed among bacteria. Thus, our EvoMining-based search may help to discover new types of secondary metabolite gene clusters in which lipid-related enzymes are involved. This work provides information about uncharacterized metabolites produced by Pseudomonas type strains, whose gene clusters have evolved in a species-specific way. Our results provide novel insight into the secondary metabolism of Pseudomonas and will serve as a basis for the prioritization of the isolated strains. This article contains data hosted by Microreact.

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Section: Discussionsupporting

confidence: 93%

“…Likewise, it has been reported that strains belonging to the species P. aeruginosa [61, 62], P. entomophila [63] and P.…”

Section: Discussionmentioning

confidence: 99%

Unveiling the genomic potential of Pseudomonas type strains for discovering new natural products

Saati‐Santamaría

Sélem-Mójica²,

Peral-Aranega

et al. 2022

Microbial Genomics

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“…The separation and purification are mostly reported by thin layer chromatography (TLC) and preparative high-performance liquid chromatography (HPLC) which have good separation resolutions [ 68 , 71 , 75 , 76 , 77 ]. However, other techniques are being studied, such as capillary electrophoresis, free flow electrophoresis, and the use of microporous adsorbent resins as alternatives to reduce the price, the use of organic solvents and the time required for the separation [ 68 , 70 , 78 , 79 , 80 ]. Liu et al [ 78 ] applied capillary zone electrophoresis to separate and quantify PCA and 2-OH-PHZ from the culture broth of P. chlororaphis GP72, after an extraction step, offering a rapid and sensitive detection.…”

Section: Extraction and Purification Of Phenazinesmentioning

confidence: 99%

“…TLC, HPLC, and UV-visible spectroscopy have been used by several authors to detect and characterize phenazines [ 13 , 20 , 32 , 69 , 72 , 76 , 79 ]. Further, structural characterization and identification of phenazines are performed, after extraction, separation, and purification, using mass spectroscopy (MS, liquid, or gas), nuclear magnetic resonance (NMR, 1 H and 13 C) ( Figure 5 ), and Fourier Transform Infra-Red spectroscopy (FTIR) ( Figure 6 ) [ 69 , 72 , 73 , 74 , 76 , 78 ].…”

Section: Phenazine Characterizationmentioning

confidence: 99%

Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

et al. 2023

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Phenazines are a large group of heterocyclic nitrogen-containing compounds with demonstrated insecticidal, antimicrobial, antiparasitic, and anticancer activities. These natural compounds are synthesized by several microorganisms originating from diverse habitats, including marine and terrestrial sources. The most well-studied producers belong to the Pseudomonas genus, which has been extensively investigated over the years for its ability to synthesize phenazines. This review is focused on the research performed on pseudomonads’ phenazines in recent years. Their biosynthetic pathways, mechanism of regulation, production processes, bioactivities, and applications are revised in this manuscript.

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“…A low-intensity zone of yellow luminescence (Rf 0.23), also visible on TLC of the P. chlororaphis BZR 245-F bacteria supernatant, was observed. Phenazines are simple redox heterocyclic brightly colored pigmented nitrogen-containing compounds with an antibiotic activity [ 22 , 23 ]. The intense zone with dark blue absorption (Rf 0.90) corresponded to that the phenazine standard solution (Merck, Darmstadt, Germany).…”

Section: Resultsmentioning

confidence: 99%

New Pseudomonas Bacterial Strains: Biological Activity and Characteristic Properties of Metabolites

et al. 2023

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This paper investigates the antagonistic and plant growth promotion activity of the new indigenous bacteria antagonist strains P. chlororaphis BZR 245-F and Pseudomonas sp. BZR 523-2. It was found that on the 10th day of cultivation, BZR 245-F and BZR 523-2 exhibit an antagonistic activity against F. graminearum at the level of 59.6% and 15.1% and against F. oxysporum var. orthoceras at the level of 50.2% and 8.9%, respectively. Furthermore, the BZR 523-2 strain stimulated the growth of winter wheat seedlings more actively than the BZR 245-F strain. When processing seeds of winter wheat, Pseudomonas sp. BZR 523-2 indicators were higher than in the control: plant height increased by 10.3%, and root length increased by 18.6%. The complex characteristic properties of the metabolite were studied by bioautography and HPLC-MS. Bioautography proved the antifungal activity of phenazine nature compounds synthesized by the new bacterial strains. We qualitatively and quantitatively analyzed them by HPLC-MS analysis of the strain sample metabolites. In the BZR 245-F sample, we found more phenazine compounds of various types. Their total phenazine concentration in the BZR 245-F was more than five times greater than in the BZR 523-2. We defined crucial differences in the quantitative content of the other metabolites. Despite the difference between new indigenous bacteria antagonist strains, they can be used as producers of effective biopesticides for sustainable agriculture management.

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Extraction, purification and characterization of phenazine from Pseudomonas aeruginosa isolate of wastewater sources: a panacea towards clinical pathogens

Cited by 7 publications

References 40 publications

Unveiling the genomic potential of Pseudomonas type strains for discovering new natural products

Unveiling the genomic potential of Pseudomonas type strains for discovering new natural products

Recent Developments in the Biological Activities, Bioproduction, and Applications of Pseudomonas spp. Phenazines

New Pseudomonas Bacterial Strains: Biological Activity and Characteristic Properties of Metabolites

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