Extraction Process of Essential Oil from Plectranthus amboinicus Using Microwave-Assisted Hydrodistillation and Evaluation of It’s Antibacterial Activity

Abstract

Plectranthus amboinicus oil has many applications in pharmaceutical, medicine and cosmetic industries. Recently, new methods of extracting essential oil used have been increasingly developed to replace traditional methods. In this study, maximization of essential oils yield from P. amboinicus was studied by the combination of microwave assisted hydro-distillation (MAHD) and response surface methodology (RSM). We found that that the maximum essential oil yield was 0.1374 % with 94.38 % reliability and influencing factors such as microwave power for this process was 515 W, raw material to water ratio 1.64:1, extraction time at 100.8 min. ANOVA analysis for quadratic model also gives favourable outcome including the high determination coefficient (R2 = 0.94), significant F-value and p-value of coefficients. Under laboratory condition, the obtained yield (0.1374 %) approximated the yield predicted by the quadratic model, suggesting the reasonable soundness of the employed model and RSM when it comes to optimizing the parameters of extraction. In addition, considerable antibacterial activities of extracted essential oil against four bacteria cultures, in particular, S. aureus was recognized.

Antimicrobial Activity from Species Plectranthus amboinicus (Lour.) Spreng, a Review

“…In Agar diffusion assays, drugs with antimicrobial potential show a growth inhibition halo with diameter proportional at its antimicrobial potential and, strong antimicrobial potentials are considered when halos with at least 15 mm of diameter are achieved [20]. According to with this parameter, P. amboinicus essential oils have strong antimicrobial activity against several strains as Staphylococcus aureus [21][22][23][24][25][26], MRSA, S. epidermidis, Enterococcus faecalis [25], Aeromonas caviae [21], Proteus vulgaris [21],…”

“…For studies raise and in many cases, the growth inhibition potential of P. amboinicus essential oil was higher than standard drugs as [22,26], where P. amboinicus essential oil showed most efficiency that than the drugs Cefepime and Gatifloxacin [22]. Another study showed higher inhibition zones for the essential oil in relation to vancomycin against gram-positive bacteria and cefotaxime against E. coli [25].…”

Effects of Various Processing Parameters on Polyphenols, Flavonoids, and Antioxidant Activities of Codonopsis javanica Root Extract

“…The selected experiment design was Central Composite Design, and the experimental matrix was built using Design-Expert 11.0 software. [28][29][30][31] Antioxidant Activity of CJR Extract Test for radical scavenging activity using the DPPH method. The extract was first diluted to a reasonable concentration.…”

Extraction process optimization and characterization of the Pomelo (Citrus grandis L.) peel essential oils grown in Tien Giang Province, Vietnam

“…On the contrary, the large peel sizes will yield lesser oil due to small surface area in contact with the solvent. Therefore, grind peels have been chosen in subsequent investigations (Dao et al, 2019b;.…”