Extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from  Glycine soja

Jing, Changliang; Yuan, Yuan; Tang, Qi; Zou, Ping; Li, Yiqiang; Zhang, Chengsheng

doi:10.1016/j.ijbiomac.2017.05.186

Cited by 20 publications

(7 citation statements)

References 28 publications

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“…A UV-visible spectrophotometer (DB-20 R, Dynamica, Australia) was used to evaluate the absorbance of each solution at λ = 490 nm. The standard curve was drawn using a polysaccharide concentration (μg/mL) as the abscissa and the absorbance value as the ordinate [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Optimization of trypsin extraction technology of Allium cepa L. polysaccharide by response surface methodology and the antitumor effects through immunomodulation

Yu-bin

Wang

2021

Bioengineered

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The trypsin-assisted extraction of polysaccharides from Allium cepa L. was optimized using the response surface methodology (RSM). The optimum extraction conditions were extraction temperature, extraction time, extraction pH, and enzyme amount of 37.16°C, 180 min, 8.57, and 5.16%, respectively. Under the optimized conditions, the yield of A. cepa L. polysaccharides (ACP) reached 9.69%, which was comparable with the predicted yield (9.73%). Mid-and high-dose ACP significantly inhibited the tumor growth (43.93%) and the tumor inhibition percentage (38.05%), which were more than 30%. The ACP could extend the survival time of H 22 ascites tumor-bearing mice. Furthermore, the ACP could reduce the thymus and the spleen atrophy and significantly promoted the Con A-induced proliferation of splenocytes and elevated the serum IFN-γ and IL-2 levels. Therefore, the ACP could inhibit the tumor growth in tumor-bearing mice and regulated the immune function of mice. Practical Applications The trypsin-assisted extraction has high efficiency, is carried out through the polysaccharide extraction and the deproteinization at the same time, and is more convenient and fast than traditional methods. No detailed study on the optimization of the trypsin extraction of onion polysaccharides is available. Thus, this experiment aims to use the BBD (4 factors and 3 levels) to optimize the roles of extraction temperature, extraction time, extraction pH, and amount of enzyme on the yield of polysaccharides obtained from the fruit of A. cepa L. In addition, when looking for high-quality biological functional principles for the pharmaceutical industry, the antitumor activity of ACP was evaluated. A. cepa L. is one of the most widely cultivated and consumed crops worldwide. Polysaccharides are the main active ingredient, and studies have shown that a high intake of Allium vegetables is associated with reduced risk of cancers.

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Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Optimization of trypsin extraction technology of Allium cepa L. polysaccharide by response surface methodology and the antitumor effects through immunomodulation

Yu-bin

Wang

2021

Bioengineered

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“…The analysis of variance (ANOVA) was performed to evaluate the predictive model and the variables. As shown in Table 2, the quadratic regression model for the MAE method has a high F-value (44.36) and a very low p -value ( p < 0.0001), indicating that the fitness of the model was extremely significant [17]. Furthermore, the lack of fit for the F-value of 3.55 and p -value of 0.1265 ( p > 0.05) implied the model equation was adequate for predicting the yield of KPS extracted by MAE [18].…”

Section: Resultsmentioning

confidence: 99%

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Han

Liu

et al. 2019

Molecules

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In order to evaluate effects of extraction techniques on the physicochemical characteristics and antioxidant activities of kiwifruit polysaccharides (KPS), and further explore KPS as functional food ingredients, both microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) were optimized for the extraction of KPS. Furthermore, the physicochemical structures and antioxidant activities of KPS extracted by different techniques were investigated. The optimal extraction conditions of UAE and MAE for the extraction of KPS were obtained by response surface methodology. Different extraction techniques significantly affected the contents of uronic acids, molecular weights, molar ratios of constituent monosaccharides, and the degree of esterification of KPS. Results showed that KPS exhibited remarkable DPPH and ABTS radical scavenging activities, and reducing power. The high antioxidant activities observed in KPS extracted by the MAE method (KPS-M) might be partially attributed to its low molecular weight and high content of unmethylated galacturonic acid. Results suggested that the MAE method could be a good potential technique for the extraction of KPS with high antioxidant activity, and KPS could be further explored as functional food ingredients.

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“…The DPPH radical scavenging activity of the soybean extracts was determined using a method described by Jing et al [13] with some modifications. In brief, extracts were dissolved in a 70% ethanol solution to prepare various concentrations, and 750 µL of a 0.1 mM ethanol solution of DPPH was mixed with various sample solutions (0-1 mg/mL).…”

Section: Antioxidant Assaysmentioning

confidence: 99%

“…where A 0 is the absorbance of the ethanol solution by DPPH without extracts, A 1 represents the absorbance of the ethanol solution by DPPH with tested extracts, and A 2 represents the absorbance of the ethanol by tested extracts. The ABTS radical scavenging activity of the soybean extracts was determined using the method described previously with some modifications [13]. The stock solution of the ABTS solution was prepared by reacting a 7.4 mM ABTS solution with 3.8 mM potassium persulfate and was then kept in the dark at ambient temperature for 16 h. For the assay, the ABTS solution was diluted with ethanol to an absorbance value of 0.7 ± 0.02 at 734 nm.…”

Section: Antioxidant Assaysmentioning

confidence: 99%

Comparison of Phenolic and Flavonoid Compound Profiles and Antioxidant and α-Glucosidase Inhibition Properties of Cultivated Soybean (Glycine max) and Wild Soybean (Glycine soja)

Chen

Wang

Yuan

et al. 2021

Plants

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Wild soybean (Glycine soja Sieb.et Zucc; WS) has been used as a traditional food in China for many years and contains significantly higher levels of isoflavones than cultivated soybean (Glycine max; CS), but the secondary metabolites, including flavonoids and the phenolic composition differences between them, remain unclear. The results showed that WS possessed significantly higher total phenolic and flavonoid content and exhibited better antioxidant and α-glucosidase inhibition activities as well as excellent protective effects against H2O2-induced oxidative injury in a human endothelial cell line. Through metabolomic analysis, 642 metabolites were identified, and 238 showed differential expression, with 151 upregulated and 87 downregulated. A total of 79 flavonoid compounds were identified, 42 of which were upregulated in WS. 2′-Hydroxygenistein, garbanzol, protocatechuic aldehyde, ligustilide, and resveratrol were the most discriminated compounds in WS. The metabolic pathway analysis of differential metabolites related to the biosynthesis of flavonoids and phenolic acids were the biosynthesis of phenylpropanoids, flavonoids, isoflavonoids, flavones, and flavonols. This study substantially elucidated differences in the content of flavonoids and biological activities between WS and CS, which is useful information for the effective utilization of these two black soybean species in food processing.

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Extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from Glycine soja

Cited by 20 publications

References 28 publications

RETRACTED ARTICLE: Optimization of trypsin extraction technology of Allium cepa L. polysaccharide by response surface methodology and the antitumor effects through immunomodulation

RETRACTED ARTICLE: Optimization of trypsin extraction technology of Allium cepa L. polysaccharide by response surface methodology and the antitumor effects through immunomodulation

Extraction Optimization, Physicochemical Characteristics, and Antioxidant Activities of Polysaccharides from Kiwifruit (Actinidia chinensis Planch.)

Comparison of Phenolic and Flavonoid Compound Profiles and Antioxidant and α-Glucosidase Inhibition Properties of Cultivated Soybean (Glycine max) and Wild Soybean (Glycine soja)

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