Extraction optimization, physicochemical properties and antioxidant and hypoglycemic activities of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves

Wu, Hongyan; Li, Mingming; Yang, Xu; Wei, Qing; Sun, Lingziyin; Zhao, Jiangchao; Shang, Hongmei

doi:10.1016/j.ijbiomac.2020.09.198

Cited by 45 publications

(27 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It could be estimated that the content of water in DES and the extraction temperature had more positive effects on the extraction yield of LLPs than that of the extraction time and the liquid-solid ratio, respectively. Generally, the shape of the contour plot is elliptical, suggesting that the mutual interaction between the two extraction parameters is notable [21]. While shape of the contour plot is circular, indicating that the mutual interaction between the two extraction parameters is not significant [21].…”

Section: Box-behnken Design Analysismentioning

confidence: 99%

“…The extraction temperature was another important parameter for polysaccharide extraction. The extraction yield of LLPs gradually increased with the increase of extraction temperature from 60 • C to 90 • C, and slightly decreased at the temperature of 100 • C. This phenomenon suggested that a relatively high extraction temperature could reduce the transfer resistance, decrease the viscosity of extraction solvent, and increase the solubility of LLPs [16,21]. However, an excessively high extraction temperature may cause the degradation of LLPs to decrease the extraction yield [16,21].…”

Section: Single Factor Experimental Analysismentioning

confidence: 99%

“…Furthermore, the extraction yield of LLPs increased with the increase of extraction time from 60 to 120 min, while a decreasing was found after 120 min. This result might be caused by the hydrolysis of LLPs under a long extraction time [21]. Finally, the optimal extraction conditions of single factor experiment were measured as follows, 60% of water content in DES, temperature of 90 • C, liquid-solid ratio of 30 mL/g, and time of 120 min.…”

Section: Single Factor Experimental Analysismentioning

confidence: 99%

“…Generally, the shape of the contour plot is elliptical, suggesting that the mutual interaction between the two extraction parameters is notable [21]. While shape of the contour plot is circular, indicating that the mutual interaction between the two extraction parameters is not significant [21]. As shown in Figure 3, significant interaction effects between the content of water in DES and the extraction temperature, the extraction temperature and the extraction time, and the content of water in DES and the liquid-solid ratio were observed visually [21], which were the same as shown in Table 2.…”

Section: Box-behnken Design Analysismentioning

confidence: 99%

See 3 more Smart Citations

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Feng

Huang

et al. 2021

Foods

View full text Add to dashboard Cite

Lotus leaves are often discarded as byproducts in the lotus industry. Polysaccharides are regarded as one of the essentially bioactive components in lotus leaves. Therefore, in order to promote the application of lotus leaves in the functional food industry, the deep eutectic solvent (DES) assisted extraction of polysaccharides from lotus leaves (LLPs) was optimized, and structural and biological properties of LLPs extracted by DES and hot water were further investigated. At the optimal extraction conditions (water content of 61.0% in DES, extraction temperature of 92 °C, liquid-solid ratio of 31.0 mL/g and extraction time of 126 min), the maximum extraction yield (5.38%) was obtained. Furthermore, LLP-D extracted by DES and LLP-W extracted by hot water possessed the same sugar residues, such as 1,4-α-D-GalAp, 1,4-α-D-GalAMep, 1,3,6-β-D-Galp, 1,4-β-D-Galp, 1,5-α-L-Araf, and 1,2-α-L-Rhap, suggesting the presence of homogalacturonan (HG), rhamnogalacturonan I and arabinogalactan in both LLP-W and LLP-D. Notably, LLP-D was much richer in HG fraction than that of LLP-W, suggesting that the DES could assist to specifically extract HG from lotus leaves. Additionally, the lower molecular weight and higher content of uronic acids were observed in LLP-D, which might contribute to its much stronger in vitro antioxidant, hypoglycemic, and immunomodulatory effects. These findings suggest that the optimized DES assisted extraction method can be a potential approach for specific extraction of acidic polysaccharides with good bioactivities from lotus leaves for applications in the functional food industry.

show abstract

Section: Box-behnken Design Analysismentioning

confidence: 99%

Section: Single Factor Experimental Analysismentioning

confidence: 99%

Section: Single Factor Experimental Analysismentioning

confidence: 99%

Section: Box-behnken Design Analysismentioning

confidence: 99%

See 2 more Smart Citations

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Feng

Huang

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…Several studies have postulated that the protein or peptide moiety in polysaccharides is partly responsible for their radical scavenging effect (Wang et al, 2016). Additionally, polysaccharides with lower molecular weights have more exposed reducing ends with which to accept and eliminate free radicals than polysaccharides with higher molecular weights; thus, polysaccharides with lower molecular weights might possess better antioxidant activity (Wu et al, 2020). The expression of genes in the polysaccharide biosynthesis pathway differs at different developmental stages, which may lead to different molecular weights of polysaccharides at different developmental stages, thus, in turn, leading to different antioxidant activities.…”

Section: Antioxidant Activities Of Polysaccharidementioning

confidence: 99%

Transcriptome analysis associated with polysaccharide synthesis and their antioxidant activity in Cyclocarya paliurus leaves of different developmental stages

Lin

Chen²,

Wang³

et al. 2021

PeerJ

View full text Add to dashboard Cite

Background Cyclocarya paliurus (Batal.) Iljinskaja is a common endemic tree species and used as a Chinese medicine. The main active components in the leaves of this plant are polysaccharides. However, the temporal patterns of gene expression underlying the synthesis of polysaccharides in C. paliurus at different leaf developmental stages and its relationship with the polysaccharide content and antioxidant activities has not been reported to date. Methods RNA-seq was used to investigate the biosynthesis pathway of polysaccharides at the four developmental stages of C. paliurus leaves. The content and the antioxidant activities of polysaccharides were measured with typical biochemical methods and the identified correlations were statistically evaluated. Results Sixty-nine differentially expressed genes were found in the leaves during different developmental stages of C. paliurus. These are associated with glycosyltransferases and belong to 18 families. During different developmental stages of C. paliurus, the polysaccharide content first increased and then decreased, and the UDP-glucose 4-epimerase gene was found to be significantly positively correlated with the polysaccharide content. The clearance rates of DPPH radicals, superoxide anion radicals, hydroxyl radicals, and the reducing power of polysaccharides in the leaves of C. paliurus at different developmental stages showed a dose-dependent relationship with the concentration of polysaccharides. Conclusions The smallest fully expanded leaves are suitable for high-quality tea, and leaves with sizes below the largest fully expanded leaves are suitable for industrial production of polysaccharides.

show abstract

Optimization of the Extraction Process and Antioxidant Activity of Polysaccharide Extracted from Centipeda minima

Yang

Fan

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

The purpose of this study is to optimize the extraction process and study antioxidant activity of Polysaccharide extracted from Centipeda minima. The Box-Behnken design-response surface methodology was adopted to optimize the extraction process of polysaccharides from Centipeda minima. We purified the crude polysaccharides from Centipeda minima, as well as determined the purity, monosaccharide composition, and molecular weight of the purified fraction. Fourier transform infrared spectrometer (FT-IR) and scanning electron microscopy (SEM) were used to analyze the structural features of the polysaccharides. Further, we investigated the antioxidant activities of different fractions of polysaccharides. Consequently, the results showed that the optimum extraction conditions for polysaccharides were: a liquid-solid ratio of 26 mL/g, extraction temperature of 85.5 °C, and extraction time of 2.4 h. Moreover, the yield of polysaccharides measured under these conditions was close to the predicted value. After purification, we obtained four components of Centipeda minima polysaccharides (CMP). The purity, monosaccharide composition, molecular weight, and structural characteristics of CMP were different, but with similar infrared absorption spectra. CMP exhibited a typical infrared absorption characteristic of a polysaccharide. Besides, CMP displayed good antioxidant activity, with potential to scavenge DPPH radical, hydroxyl radical, and superoxide radical. Therefore, this study provides a reference for future research on the structure and biological activity of CMP, and lays a theoretical foundation for food processing and medicinal development of CMP.

show abstract

Extraction optimization, physicochemical properties and antioxidant and hypoglycemic activities of polysaccharides from roxburgh rose (Rosa roxburghii Tratt.) leaves

Cited by 45 publications

References 44 publications

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Deep Eutectic Solvent-Assisted Extraction, Partially Structural Characterization, and Bioactivities of Acidic Polysaccharides from Lotus Leaves

Transcriptome analysis associated with polysaccharide synthesis and their antioxidant activity in Cyclocarya paliurus leaves of different developmental stages

Optimization of the Extraction Process and Antioxidant Activity of Polysaccharide Extracted from Centipeda minima

Contact Info

Product

Resources

About