Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid

Abstract

In this study, polysaccharide–rich Nymphaea hybrid extracts (NHE) were obtained using the ultrasound-assisted cellulase extraction (UCE) method optimized by response surface methodology (RSM). The structural properties and thermal stability of NHE were characterized by Fourier-transform infrared (FT–IR), high–performance liquid chromatography (HPLC) and thermogravimetry–derivative thermogravimetry (TG–DTG) analysis, respectively. Moreover, the bioactivities of NHE, including the antioxidant, anti–inflammatory, whitening and scratch healing activities were evaluated by different in vitro assays. NHE conveyed a good ability to scavenge against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and inhibit the hyaluronidase activity. NHE can effectively protect the HaCaT cells against oxidative damage by inhibiting the intracellular reactive oxygen species (ROS) production in the H2O2 stimulation assays and promoting the proliferation and migration in the scratch assays. In addition, NHE was proven to inhibit melanin production in B16 cells. Collectively, the above results seem to be the evidence needed to promote the potential of NHE to be regarded as a new functional raw material in the cosmetics or food industries.

Molecular understanding of the therapeutic potential of melanin inhibiting natural products

“…Furthermore, NHE exhibited some antioxidant activity, although its scavenging of DPPH radicals was found to be weaker than that of VC (with IC 50 values of 0.0817 and 0.008 mg mL −1 , respectively). 76…”

Optimization of Three Extraction Methods and Their Effect on the Structure and Antioxidant Activity of Polysaccharides in Dendrobium huoshanense

“…The DTG (red line) of DHP-UM (Figure 2E) exhibited three weight loss stages, while DHP-H (Figure 2F) exhibited two weight loss stages. The pyrolytic curves of DHPs tended to be similar in the first stage of weight loss (15.05-120 • C), which might be attributed to the evaporation of the free water and binding water to the polysaccharides [27,28] -H), a significant decrease in the weight of polysaccharides was observed, potentially due to the depolymerization and decomposition of polysaccharides [27,28] In addition, during the third phase between 353.67 and 370.25 • C (DHP-UM), there was a reduction in weight, potentially caused by the decomposition of other macromolecules [29]. Consequently, these results showed that DHPs had relative thermal stability below 313.92 • C. It is worth mentioning that the thermal stability of DHP-E and DHP-UM is weaker than that of DHP-H.…”

Preparation, Characterization, and Antioxidant Activities of Extracts from Amygdalus persica L. Flowers

“…Polysaccharides, as abundant biomacromolecules in nature, exhibit a wide range of biological activities, attracting significant attention. It has been reported that plant polysaccharides from wild-growing plants like Nymphaea hybrid, Lycopus lucidus, and Ganoderma lucidum, demonstrate remarkable natural antioxidant properties without the toxicity associated with chemically synthesized antioxidants [17][18][19]. Furthermore, many plant polysaccharides possess anti-cancer, anti-inflammatory, and anti-aging properties, primarily attributed to their antioxidant effects [20].…”