2022
DOI: 10.1002/jctb.7209
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Extraction of β‐galactosidase from Saccharomyces fragilisIZ 275 grown in cheese whey

Abstract: BACKGROUND The enzyme β‐galactosidase (EC 3.2.1.2.3) obtained from yeast is produced intracellularly. Cell extraction consists of the treatment of a cell suspension with chemical agents, thus obtaining the enzyme with biological activity. After obtaining the extracted enzyme, it is concentrated by ultrafiltration and dried. Lyophilization can be used in this step. The objective of this work was to study the production and extraction of β‐galactosidase from Saccharomyces fragilis IZ 275 using lactose from chees… Show more

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