Extraction of viral nucleic acids: Comparison of five automated nucleic acid extraction platforms

Verheyen, Jens; Kaiser, Rolf; Bozic, Michael; Timmen-Wego, Monika; Maier, Barbara K.; Kessler, Harald H.

doi:10.1016/j.jcv.2012.03.008

Cited by 58 publications

(41 citation statements)

References 28 publications

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“…The screening test must include a sample preparation method that ensures high recovery of nucleic acids and sufficient purity of clinical samples to control inhibitors (7,8 ). Comparative performance studies of automated extraction platforms have demonstrated the direct correlation between the performance of the extraction system and the imprecision of a molecular assay (7)(8)(9).…”

confidence: 99%

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

et al. 2014

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BACKGROUND: Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity.

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confidence: 99%

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

et al. 2014

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“…Automated methods are generally just as effective as manual methods (65 ). Many valuable comparisons of methods have been published (66,67 ) but are usually limited to only a few methods and a single sample target combination. Most comparisons demonstrate that there is not a big difference between preparation methods.…”

Section: Selecting From Current Methodsmentioning

confidence: 99%

DNA/RNA Preparation for Molecular Detection

Thatcher

2015

Clinical Chemistry

106

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BACKGROUND Effective upstream preparation of nucleic acid (NA) is important for molecular techniques that detect unique DNA or RNA sequences. The isolated NA should be extracted efficiently and purified away from inhibitors of a downstream molecular assay. CONTENT Many NA sample preparation techniques and commercial kits are available. Techniques for cell lysis and isolation or purification of NA were discovered in early NA characterization studies, evolved in the 20th century with molecular techniques, and still serve as the foundation for current methods. Advances in solid phase extraction methods with nonhazardous chemicals and automated systems have changed the way NA is prepared. Factors to consider when selecting NA preparation methods for molecular detection include lysis (from sources as diverse as human cells, viruses, bacterial spores, or protozoan oocysts), DNA vs RNA, sample background, appropriate preparation chemicals, and required detection limits. Methods are also selected on the basis of requirements for a particular application, such as sample volume or removal of inhibitors. Sometimes tradeoffs are made. SUMMARY Good automated and manual methods are available to effectively prepare NA for molecular detection in under an hour. Numerous systems are available for various applications, including techniques that are flexible for multiple sample types, are capable of processing large batches, can be performed in <10 min, or that can yield high-purity NA. When methods are selected using the most applicable combination of lysis isolation efficiency and concentration, NA preparation can be very effective, even for molecular detection of multiple targets from the same sample.

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“…CMV, EBV and adenovirus reactivation was monitored by twice-weekly DNA PCR monitoring [20]. CMV reactivation (>400 copies/ml) was treated pre-emptively with Ganciclovir.…”

Section: Pre-transplant Work-up and Supportive Carementioning

confidence: 99%

Nonmyeloablative allogeneic stem cell transplantation for chronic lymphocytic leukaemia offers the possibility of disease control with minimal morbidity and mortality—a single institution experience

Chakupurakal

Leitzke

Langerbeins³

et al. 2015

Ann Hematol

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Allogeneic stem cell transplantation is a treatment option for patients with poor risk CLL. We conducted a retrospective analysis of all CLL patients allografted at our institution, the University Hospital of Cologne, Germany. Data was collected on 40 patients from 2004 to 2012. The mean age was 54, and the majority were male (75 %). On average, the patients were diagnosed 6 years (range 2-12) prior to transplant with an average of 4 years (range 1-8) from time of first-line therapy to transplant. The remission states at the time of transplant were complete remission (CR) (n = 4), stable disease (n = 10), partial remission (n = 20) and progressive disease (n = 6). Only reduced intensity conditioning regimens were employed. The average CD34(+) cell dose was 4.16 × 10(6)/kg. Neutrophil engraftment was seen by day +17 (range 10-23) post-transplant, and 88 % achieved 95-100 % donor chimerism by day 100. Overall survival, progression-free survival and non-relapse mortality at 2 years post-transplant were 65, 52.5 and 27.5 %, respectively. A total of 51 % of patients were found to be minimal residual disease (MRD)-negative at 1 year post-transplant. Our single-centre experience confirms the valuable role of allogeneic stem cell transplantation (allo-SCT) in the treatment of poor risk CLL patients with promising long-term survival and acceptable transplant-related mortality. The advent of newer therapeutic agents should not hinder the consideration of allo-SCT for this patient cohort as it remains the only curative option for these patients.

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Extraction of viral nucleic acids: Comparison of five automated nucleic acid extraction platforms

Cited by 58 publications

References 28 publications

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples

DNA/RNA Preparation for Molecular Detection

Nonmyeloablative allogeneic stem cell transplantation for chronic lymphocytic leukaemia offers the possibility of disease control with minimal morbidity and mortality—a single institution experience

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