Extraction of trace amounts of pioglitazone as an anti-diabetic drug with hollow fiber liquid phase microextraction and determination by high-performance liquid chromatography-ultraviolet detection in biological fluids

Tahmasebi, Elham; Yamini, Yadollah; Saleh, A. K. Md. Ehsanes

doi:10.1016/j.jchromb.2009.05.033

Cited by 72 publications

(32 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As the thickness of the boundary layer (Nernst diffusion layer) decreases, extraction is higher due to the reduction caused in the resistance toward mass transport [17,18] and, since the thickness of the layer depends on the stirring speed of the solution, higher stirring speeds should be selected. Also, stirring of sample solution reduces the time required to reach thermodynamic equilibrium and induces convection in the sample solution [19]. Nevertheless, at higher stirring speeds, air bubbles are produced on the surface of the Fig.…”

Section: Influence Of Stirring Speedmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

Self Cite

View full text Add to dashboard Cite

Hollow fiber liquid-phase microextraction (HF-LPME) based on two immiscible organic solvents (n-dodecane as membrane solvent and 22 lL acetonitrile as acceptor phase) was evaluated for extraction and preconcentration of trace amounts of miconazole and clotrimazole as model azole antifungal drugs, prior to determination by gas chromatography (GC). The drugs were extracted from 24 mL sample solution into a thin layer of organic phase (n-dodecane) immobilized in hollow fiber pores, and then back-extracted into approximately 22 lL organic phase (acetonitrile) located in the lumen of the hollow fiber. After extraction, 1 lL acceptor phase was injected into a GC instrument. Under optimum conditions, limits of detection (LODs) ranged from 0.3-6.0 ng mL -1 , linear dynamic range of 1-400 ng mL -1 and relative standard deviations (RSD%) \5% were obtained. The developed method provided preconcentration factors of 639 and 496 for clotrimazole and miconazole, respectively. The applicability of the extraction method to different types of complicated matrices such as milk, plasma and urine samples was investigated. The results obtained results reveal that the proposed method is a good technique for extraction and determination of the target drugs in different matrices.

show abstract

Section: Influence Of Stirring Speedmentioning

confidence: 99%

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…As per the literature, various analytical methods such as membrane sensitive electrode [2], potentiometric [3], ultravioletspectrophotometric [4], high-performance liquid chromatographic (HPLC) methods [5][6][7][8][9][10], ultra-performance liquid chromatograph method [11], and liquid chromatography-mass spectrometry (LCMS) methods [12][13][14][15][16][17] have been reported for the determination of PG and its metabolites. The particular described methods have to sacrifice time, resolution as well as sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Sensitive Liquid Chromatography- Mass Spectrometry/Mass Spectrometry Method for Estimation of Pioglitazone, Keto Pioglitazone and Hydroxy Pioglitazone in Human Plasma

Ponnuri

Pragallapati

Ravindra³

et al. 2017

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The main objective of the work was to develop a straightforward, fast and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of pioglitazone (PG), keto pioglitazone (KPG), and hydroxy pioglitazone (HPG) in human plasma and to validate as per recent guidelines. Methods:Analyte and the internal standard (IS) were extracted from plasma through liquid-liquid extraction and chromatographed on a Xterra RP18, 100×4.6, 5 µ column using methanol: acetonitrile mixture and 10 mM Ammonium formate buffer (70:30, v/v) as the mobile phase at a flow rate of 0.7 mL/min. The API-3200 Q Trap LC-MS/MS instrument in multiple reaction monitoring mode was used for detection. Diphenhydramine was utilized as IS. Results:The linearity was established in the concentration range of 20.15-1007.58 ng/mL for PG, 20.35-1017.58 ng/mL for KPG, and 19.68-491.22 ng/mL for HPG in human plasma. All the validation parameters were well within the acceptance limits. Conclusion:A new simple LC-MS/MS method was developed for the determination of PG, KPG, and HPG in human plasma. This method can be easily applied for the estimation of pharmacokinetic parameters of PG, KPG, and HPG.

show abstract

“…Pioglitazone decreases insulin resistance in the periphery and liver, resulting in increased insulindependent glucose disposal and decreased hepatic glucose output. Few methods for the determination of pioglitazone hydrochloride were reported including voltammetry in tablets and biological fluids [3], potentiometry in tablets [4], extractive spectrophotometry in raw material and tablets [5], HPLC in pharmaceutical formulations [6][7][8][9][10][11] and in biological fluids [11][12][13][14]. Pioglitazone hydrochloride has been determined in combination with other drugs using UV-spectrophotometry [15][16][17], HPLC [15,[18][19][20][21] in combined dosage forms, HPLC in formulations and human serum [22][23][24][25] and LC-MS methods in human…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Stability Indicating HPLC Method for Quality Control of Pioglitazone Hydrochloride

2014

Can Chem Trans

View full text Add to dashboard Cite

A simple, rapid and sensitive RP-HPLC method for the quantification of pioglitazone hydrochloride in bulk drug and tablet formulation was developed and validated. Chlordiazepoxide was used as internal standard. The separation was achieved on a Nucleodur 100-5 C18 column (250 mm× 4.6 mm i.d., 5 m particle size). The mobile phase consisted of methanol-0.07 M formic acid (57:43, v/v) at a flow rate of 0.9 mL/min and detection was performed at 266 nm using photodiode array (PDA) detector. The drug was subjected to various ICH prescribed stress conditions including hydrolysis (acid and alkaline), oxidation, photolysis and thermal degradation. Degradation in acid, base and peroxide was found in range 36-45%. The proposed method was validated with respect to specificity, linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), stability, and robustness as per ICH guideline. The developed method was found to be successively applied for the quality control of pioglitazone hydrochloride in bulk drug and tablets as well as the stability indicating studies.

show abstract

Extraction of trace amounts of pioglitazone as an anti-diabetic drug with hollow fiber liquid phase microextraction and determination by high-performance liquid chromatography-ultraviolet detection in biological fluids

Cited by 72 publications

References 61 publications

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID

A Rapid and Sensitive Liquid Chromatography- Mass Spectrometry/Mass Spectrometry Method for Estimation of Pioglitazone, Keto Pioglitazone and Hydroxy Pioglitazone in Human Plasma

Development and Validation of Stability Indicating HPLC Method for Quality Control of Pioglitazone Hydrochloride

Contact Info

Product

Resources

About