Cultivated land resources are an important basis of regional sustainability; thus, it is important to determine the distribution of the cultivated land in the Northeast Asia trans-boundary area of China, Russia and Mongolia, which has a continuous geographic and ecological environment and an uneven population distribution. Extracting information about the cultivated land and determining the spatial and temporal distribution of its features in this large trans-boundary area is a challenge. In this study, we derived information about the cultivated land of the North-South indicate the presence of a greater amount of cultivated land in the south and less in the north. China owns most of the cultivated land in the transect area, followed by Mongolia and then Russia. A gradient analysis revealed a decrease of 34.16% of the cultivated land between 2000 and 2010. The amount of cultivated land decreased 22.37%, 58.93%, and 64.73% in China, Russia, and Mongolia, respectively. An analysis shows that the amount of cultivated land is primarily influenced by the various land development and protection policies in the different counties in this trans-boundary area.