Extraction of tannin by Acacia mearnsii with supercritical fluids

Pansera, Márcia Regina; Iob, Gelson Antonio; Atti-Santos, A. C.; Rossato, Marcelo; Atti-Serafini, L.; Cassel, Eduardo

doi:10.1590/s1516-89132004000600019

Cited by 28 publications

(11 citation statements)

References 5 publications

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“…The inner bark and outer bark of Angico (Anadenanthera The extract was prepared from the bark from a tree at the Pharmacy Department of the Pharmacognosy Laboratory, UFPE. After collecting, the material was air dried, milled, the powder obtained was weighed and diluted in 500 ml methanol 16 .…”

Section: Preparation Of the Extract Of Anadenanthera Colubrina Var Cmentioning

confidence: 99%

Effects of angico extract (Anadenanthera colubrina var. cebil) in cutaneous wound healing in rats

Pessoa¹,

Estevão²,

Simões³

et al. 2012

Acta Cir. Bras.

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Section: Preparation Of the Extract Of Anadenanthera Colubrina Var Cmentioning

confidence: 99%

Effects of angico extract (Anadenanthera colubrina var. cebil) in cutaneous wound healing in rats

Pessoa¹,

Estevão²,

Simões³

et al. 2012

Acta Cir. Bras.

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“…The isolated compound from P. granatum fruit peels was dissolved in appropriate solvent, applied to silica gel plates, Merck (Germany) 20×20 cm, 0.25 mm in thickness, and developed using the solvent system methanol: chloroform (2:1) v/v [15]. The separated zone was visualized spraying with 0.1 % (w/v) ferric chloride FeCl 3 .…”

Section: Thin Layer Chromatography Tlcmentioning

confidence: 99%

Isolation and Identification of Antibacterial Compound from Punica granatum L. Fruit Peels Grown in Iraq

Mahmood¹,

Mohammed²,

Al-Bayati³

2010

J.Edu.Sci.

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The aim of this study was to isolate and identify the substance responsible for the pharmacological activities of Punica granatum L. fruit peels. Tannic acid (ellagitannins, gallotannins) was isolated from pomegranate fruit husk and detected on Thin layer chromatography TLC plates in comparison with standard tannic acid that, served as a positive control. Moreover, infrared FTIR spectrometer and High Performance Liquid Chromatography HPLC analysis were used to confirm the purity and characterization of tannic acid. The isolated material was investigated for its antibacterial activity against six different pathogenic bacteria. The Gram-positive bacteria were; Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes; and Gram-negative bacteria included; Brucella melitensis, Klebsiella pneumoniae and Pseudomonas aeruginosa. Tannic acid at different concentrations (50.0, 25.0, 12.5, 6.2 mg/ml) was active against all tested bacteria and the highest inhibitory effect was observed against K. pneumoniae (zone of inhibition: 22.6 mm) using the disc diffusion method. The minimal inhibitory concentration MIC for tannic acid was determined using a broth microdilution method in 96 multi-well microtiter plates. MIC values ranged from 31.2-250.0 µg/ml, and the most promising result was observed against K. pneumoniae.

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“…Secondary metabolites such as tannins have also been reported for their potential effects on ruminants (Aerts et al 1999). They are polyphenolic compounds present in plants, foods and beverages, which are soluble in water and polar organic solvents (Pansera et al 2004). It is postulated that CT may impair vital processes such as feeding and reproduction of the parasite or may bind and disrupt the integrity of the parasites' cuticle (Niezen et al 1995).…”

Section: Discussionmentioning

confidence: 99%

The potential of Elephantorrhiza elephantina as an anthelminthic in goats

Maphosa

Masika

2012

Parasitol Res

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Elephantorrhiza elephantina Bruch. Skeels. is used by farmers in the Eastern Cape Province to control helminths in goats. An in vitro study revealed efficacy of its fractions against adult Haemonchus contortus. This study was conducted to validate efficacy of fractions in vivo, on gastrointestinal nematodes in naturally infected goats. A total of 36 goats (18 males and 18 females) between ages of 8 and 12 months were used; these were randomly assigned to six treatments (A-F). For treatments A and B, goats received Prodose orange® (Albendazole 1.92 % m/v, closantel 3.94 % mv) at 2 ml/10 kg and distilled water at 0.5 ml/kg per os, respectively, whereas those under treatments C to E received aqueous fractions of E. elephantina at concentrations of 12. 5, 25, 50 and 75 mg/ml, dosed at 2 ml/10 kg. On days 1, 28 and 56, faecal samples were collected for faecal egg counts (FEC) and larval count, blood samples taken for packed cell volume (PCV) and body weights recorded to assess weight changes. The aqueous fraction caused highest the total faecal egg count reductions (TFECR) % of 81.7 %, 96.8 % and 98.6 % at doses of 25, 50 and 75 mg/kg, respectively, on day 56. This was comparable to the commercial drug Prodose orange®, which caused TFECR% of 94.9 % at the same time. The fraction also caused reduction of Haemonchus contortus and strongyloides larvae at all dose levels, but these were not significantly different (P > 0.05) to the negative control. There was an increase in body weights of animals at dose concentrations of 25, 50 and 75 mg/kg. Animals receiving the fraction had weight increases of between 3 and 4 kg by end of experiment (over 56 days), but those drenched with Prodose orange ® gained by less than 2 kg. The fraction also increased PCV levels at all doses on days 28 and 56 and this was comparable to the group on commercial drug. The reductions of FEC and worm larvae, as well as increase in PCVs are indicative that E. elephantina posses some antihelmintic properties against gastrointestinal parasites in goats. With a minimum dose concentration of 25 mg/ml being effective, this means that farmers can use little material. Increase in weight gain revealed in this study may indicate that the plant may have some nutritional value; further study is therefore called for to validate the plant for its nutritive value.

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Extraction of tannin by Acacia mearnsii with supercritical fluids

Abstract: Studies were carried out on solvent and supercritical extraction to obtain natural tannins. The results showed that the best co-solvent to extract tannin with CO 2 supercritical was water with a concentration of 5.0% and the best trap rinse solvent was methanol.

Cited by 28 publications

References 5 publications

Effects of angico extract (Anadenanthera colubrina var. cebil) in cutaneous wound healing in rats

Effects of angico extract (Anadenanthera colubrina var. cebil) in cutaneous wound healing in rats

Isolation and Identification of Antibacterial Compound from Punica granatum L. Fruit Peels Grown in Iraq

The potential of Elephantorrhiza elephantina as an anthelminthic in goats

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