Extraction of Soybean Oil from Single Cells

Abstract

Single cells prepared from autoclaved soybeans and cellulase treatment of the cells were effective in digesting the cell walls of and extracting the oil from soybeans. The first cell wall of the soybean single cell was completely removed using cellulases; the thin and transparent second cell wall of the cell was swollen. Oil in the cell formed spherical or hemispherical oil drops, and oil leaking from the oil bodies was observed. The oil was almost retained within the second cell wall. Water-extractable substances were obtained at approximately >60% of the weight. Flotation of oil drops by centrifugation was easily done. Ambient n-hexane extraction was also possible; however, residual oil remained in the oil bodies. Protease or peptidase digested the structure of the oil bodies; however, separation of the oil and the hydrolysates was impossible. The oil from the oil bodies was obtained effectively (>85%) by pressing the single cells and/or cellulase-treated single cells.

Response surface optimization of ultrasound-assisted oil extraction from autoclaved almond powder

“…Therefore, the breaking of all the cells is needed before the oil is obtained, and cellulase, hemicellulase and protease can generally assist in the extraction recovery (Kasai, Imashiro, & Morita, 2003). However, after the cell wall is broken, the protein and the oil seep out simultaneously, forming an emulsion of oil and water that are difficult to isolate from each other (Kasai et al, 2003). To solve this problem, Kasai et al (2003) developed an efficient method with the pretreatment using autoclaving and enzymes to prepare single cell for the soybean oil extraction.…”

“…However, after the cell wall is broken, the protein and the oil seep out simultaneously, forming an emulsion of oil and water that are difficult to isolate from each other (Kasai et al, 2003). To solve this problem, Kasai et al (2003) developed an efficient method with the pretreatment using autoclaving and enzymes to prepare single cell for the soybean oil extraction. However, there is no report, to the best of our knowledge, on the almond oil extraction with the help of the autoclaving treatment.…”

Are Agricultural Plant Products and the Residues Indigestive Fibers?

“…1) Therefore, our method and results would be useful in digesting okara, if the secondary cell wall can be digested. We tried to adapt these results 1) for okara digestion and studied the composition of the secondary cell wall and the selection of effective enzymes for the digestion of the secondary cell wall. Enzyme selection for the digestion of the secondary cell wall was carried out, and Pectinex (food processing enzyme from Novozyme) was selected as the desired enzyme in the result.…”

“…Information on the enzymatic soluble structure, its localization, and or digestible components and the indigestible components were also reported. 1) Composition of remaining cellulase-digested okara.…”

“…1,2) This extract would be the residual of soybean milk and the materials between cells as determined by HPLC analysis and staining results. 3) It is noted that sugar and protein were digested and solubilized as a result, although cellulase digestion was to digest the primary cell walls of okara.…”