Extraction of SOD from Alfalfa with Ultrasonic Assisted

Abstract

In this study, the new technological parameters have been optimized for running Single factor experiment and orthogonal design test. Furthermore, the optimal extractible condition of SOD activity from Alfalfa was determined by the assay of NBT photoreduction method. The results reveal that when the ratio of liquor to material is 1 to 10; pH value of phosphate buffer solution is 7.5; ultrasonic power is 400 w, therefore, the SOD activity of Alfalfa is up to 66.54 U/mL. In addition, we characterized the activity analysis of Alfalfa proteins using heat denaturation, ammonium sulfate precipitation and ultraviolet spectroscop. The results showed that the protein removal rate is approximate 91.70 % and SOD specific activity is 6.5 times. Based on the above results, we reached a preliminary estimate that the SOD from Alfalfa is a kind of Cu/Zn-SOD.