Extraction of Se species in buckwheat sprouts grown from seeds soaked in various Se solutions

Selenium and Its Species Distribution in Above-ground Plant Parts of Selenium Enriched Buckwheat (Fagopyrum esculentum Moench)

“…18 However, none of the modifications used gave a major improvement in the soluble Se content obtained.…”

“…15 A study of the Se species distribution in onions indicated that Se(VI) is mostly transported by an apoplastic mechanism, inert transport without infiltration into the cell and without transformation to other chemical forms, while more toxic Se(IV) crosses the cell membrane (symplastic transport) and infiltrates into the cell, where it is reduced to less hazardous seleno amino acid forms. 16 An investigation of Se speciation and its content has already been performed on different plants, including bean seeds, 17 buckwheat seeds and leaves, 13,17 buckwheat sprouts, 18 potato tubers, 19 various cereals 20 and others. Se in Se-enriched plants is most often present in the form of SeMet or SeMeSeCys, and in the form with which the plants were treated.…”

Atomic spectrometry update. Elemental speciation

“…A variety of methods for the extraction of Se species from buckwheat sprouts have been evaluated. 55 The extractants were water, phosphate buffer, 0.1, 0.2, 0.3 M HCl, the enzyme protease XIV on its own or in combination with cellulase, amylase or lipase. The best extractants were found to be hydrolysis with 0.3 M HCl or protease; however, only approximately one-quarter to one-half of the relevant species were accounted for, although the species in the extracts were stable for 30 d. The UAE of Br and I species from atmospheric aerosols has been optimized.…”

Chromatographic Analysis of Selenium Species

“…Cuderman et al [30] examined different extraction media to identify selenium species in buckwheat. The optimal extraction efficiencies were obtained by hydrolysis with HCl, followed by breaking the cells with liquid nitrogen and then enzymatic hydrolysis with protease XIV.…”