Extraction of proteins with temperature sensitive and reversible phase change of ionic liquid/water mixture

Kohno, Yuki; Saita, Shohei; Murata, Kenichi; Nakamura, Nobuhumi; Ohno, Hiroyuki

doi:10.1039/c0py00364f

Cited by 93 publications

(81 citation statements)

References 31 publications

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“…Furthermore, these solutions may also change their solvation behavior as a function of temperature offering a nice route to extraction and separation by precipitation [107]. …”

Section: Through the Eyes Of The Proteinmentioning

confidence: 99%

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

2017

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In the last two decades, while searching for interesting applications of ionic liquids as potent solvents, their solvation properties and their general impact on biomolecules, and in particular on proteins, gained interest. It turned out that ionic liquids are excellent solvents for protein refolding and crystallization. Biomolecules showed increased solubilities and stabilities, both operational and thermal, in ionic liquids, which also seem to prevent self-aggregation during solubilization. Biomolecules can be immobilized, e.g. in highly viscous ionic liquids, for particular biochemical processes and can be designed to some extent by the proper choice of the ionic liquid cations and anions, which can be characterized by the Hofmeister series.

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“…Furthermore, these solutions may also change their solvation behavior as a function of temperature offering a nice route to extraction and separation by precipitation [107]. …”

Section: Through the Eyes Of The Proteinmentioning

confidence: 99%

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

2017

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“…[25] We have recently reported that horse heart cytochrome c (Cyt.c) and several other proteins have been extracted in a moment from aqueous phase to the IL phase using LCST behaviour of the IL/water mixture. [26] Since the phase change was controlled by changing temperature for a few degrees, the LCST-type phase response of IL/water mixtures permits the design of novel solvents for extraction and separation of a wide variety of materials including biopolymers. However, there were only a few ILs found to show LCST-type phase transition after mixing with water.…”

Section: Introductionmentioning

confidence: 99%

Material design of ionic liquids to show temperature-sensitive LCST-type phase transition after mixing with water

et al. 2011

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Phosphonium cations bearing different alkyl chains were coupled with several common anions so as to prepare ionic liquids (ILs) with diverse hydrophobicity. A temperature-driven phase behaviour of the mixture of various ILs and water has been examined. A few ILs were found to exhibit temperature-sensitive lower critical solution temperature (LCST)-type phase transition after mixing with water. The phase separation temperature (T c ) of the IL/water mixtures depended strongly on the hydrophobicity of the component ions as well as mixing ratio. The number of water molecules per ion pair in the IL phase (m water ) increased dramatically upon cooling. The temperature dependence of this parameter was found to be useful to predict the possibility of the ILs to show the LCST-type phase behaviour after mixing with water. Since the value of m water depended on the ion structure, especially on the hydrophobicity, the T c was accurately set out by suitably mixing two ILs with different hydrophobicity.

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“…This might also explain the behavior of the enzyme when water is added in both ILs (Asakawa et al, 2015). Enzymes usually require a certain amount of water in order to function in a particular reaction (Kohno, Saita, Murata, Nakamura, & Ohno, 2011).…”

Section: Determination Of Kinetics Parameters Of Tr-cel With Cmc and mentioning

confidence: 98%

Hydrolysis Kinetics of Oil Palm Empty Fruit Bunch in Ionic Liquids and Cellulase Integrated System

Elgharbawy¹,

Alam²,

Moniruzzaman³

et al. 2019

IJC

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Ionic liquids (ILs) are developing as potential solvents in lignocellulose solvation, which enables cellulase accessibility into the substrate. Nevertheless, ILs could result in enzyme deactivation because of the high polarity. Therefore, developing a system of ILs-compatible cellulase (IL-E) to promote lignocellulose conversion into sugars is a challenge in ILs applications. This study used an IL-E to attain high conversion yield of sugars from oil palm empty fruit bunch (EFB). Cellulase (Tr-Cel) from Trichoderma reesei was stable in the ILs, 1-ethyl-3-methyl imidazolium diethyl phosphate [EMIM]DEP and choline acetate [Cho]OAc. The inhibition and deactivation of cellulase were evaluated using the model substrate, carboxymethyl cellulose (CMC) and EFB as a lignocellulosic material to assess the hydrolytic activity. The enzyme kinetics revealed that [Cho]OAc acted as a noncompetitive inhibitor. Additionally, [EMIM]DEP may not be considered as an inhibitor as it increases the Vmax and does not significantly affect the KM. In both cases, the study proved that IL did not result in a severe loss of cellulase activity, which is a promising outcome for one-pot hydrolysis of lignocellulosic materials.

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Extraction of proteins with temperature sensitive and reversible phase change of ionic liquid/water mixture

Cited by 93 publications

References 31 publications

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

Proteins in Ionic Liquids: Current Status of Experiments and Simulations

Material design of ionic liquids to show temperature-sensitive LCST-type phase transition after mixing with water

Hydrolysis Kinetics of Oil Palm Empty Fruit Bunch in Ionic Liquids and Cellulase Integrated System

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