Extraction of protein from skim natural rubber latex using PEG as a surfactant via low speed centrifugation and continuous flow

Abstract

Protein extractions from skim natural rubber latex using 3 %w/v polyethylene glycol (PEG6000) via both low speed centrifugation and continuous flow were investigated. In centrifugal extraction, when the speed was 1000 rpm, the extractable protein (EP) content in serum increased with processing time from 5 to 30 min and when the time was fixed at 5 min, EP content increased with centrifugal speed. In addition, further washing deproteinized chips with 2 %w/v SDS solution could remove proteins with efficiencies corresponding to the efficiencies of protein removals in latex phase, implying the role of PEG in protein reduction in both steps. In continuous flow extraction, EP content increased with increasing Reynolds number or increasing mean residence time of the flow to a maximum and then dropped. The efficiencies of the centrifugal extraction and continuous flow extraction were 55.2 and 33.7%, respectively.

Recovery of bacterioruberin and proteins using aqueous solutions of surface-active compounds

“…As surfactants and tensioactive ILs are amphiphilic molecules, 33 they become effective solvents in the extraction of both more hydrophilic compounds, e.g. proteins, 10,[34][35][36][37] and more hydrophobic molecules, [37][38][39][40][41] such as bacterioruberin. Furthermore, their use has several advantages, namely milder process conditions while using water as the main solvent, which could also be attractive for cell disruption, as most haloarchaea lyse rapidly when suspended in water.…”

Preparation of skim natural rubber and polypropylene blends via melt blending: A study on physico-mechanical properties

“…Numerous works have been carried out attempting value addition to SNR suppressing the inferior properties of both skim latex and SNR, without much adaptability in the industry. [2][3][4][6][7][8][9][10] It may be convenient to modify SNR rather than skim latex as the latter predominantly is a highly alkaline and diluted dispersion of 5-10% (w/w) NR particles in an aqueous medium. On the other hand, SNR is available in the dry form containing around 85% of NR hydrocarbons.…”

Adjusting the Low Molecular Weight of Hydroxyethyl Cellulose and Applied to Extract Proteins in Natural Rubber Latex

“…Another method is to use watersoluble synthetic polymers such as polyvinyl pyrrolidone (PVP) [10], polyethylene glycol (PEG) 6000 [3,11] to obtain the skimmed NRL with a protein content of 0,67 mg/g of rubber. In Vietnam, protein-free natural rubber latex has been prepared by incubation with urea in SDS surfactant solution [4,12] or SDS combining with magnetic coated alumina (Fe 3 O 4 @Al 2 O 3 ) nanoparticle [13] or only Fe 3 O 4 @Al 2 O 3 nanoparticle [13]. Low protein NRL was also prepared using synthetic polymers such as PVA with molecular weight (Mw) ~22,500 and 90,000 g/mol, PVP with Mw 40,000 and 360,000 g/mol at a concentration of 3 phr (part per hundred of rubber) [14].…”