Extraction of polysaccharides from edible mushrooms: Emerging technologies and recent advances

Leong, Yoong Kit; Yang, Fan; Chang, Jo‐Shu

doi:10.1016/j.carbpol.2020.117006

Cited by 162 publications

(98 citation statements)

References 119 publications

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“…In addition, extraction of FIII-2-b with these solvents are generally performed after hot water extraction followed by successive extraction steps to maximize polysaccharide recovery [41]. Therefore, the lengthy extraction period is another limitation of alkaline extraction of polysaccharides [41]. In the present study, the extraction rate of FIII-2-b from the fruiting body of ABM was less than 1%.…”

Section: Preparation Of Abm Fruiting Body Extracts At Subcritical Conditions and Increasing Temperaturesmentioning

confidence: 69%

“…These solvents pose major hazards during large-scale extraction. In addition, extraction of FIII-2-b with these solvents are generally performed after hot water extraction followed by successive extraction steps to maximize polysaccharide recovery [41]. Therefore, the lengthy extraction period is another limitation of alkaline extraction of polysaccharides [41].…”

Section: Preparation Of Abm Fruiting Body Extracts At Subcritical Conditions and Increasing Temperaturesmentioning

confidence: 99%

“…Water has a high dielectric constant and a high polarity at ambient conditions. A high temperature lowers the dielectric constant, the polarity and weakens the hydrogen bonds of water, thereby water is converted to a less-polar solvent [41]. The solubility, extraction rate and activity of less polar compounds improve when the temperature of the subcritical water is increased [40].…”

Section: Introductionmentioning

confidence: 99%

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Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor

Yasuma¹,

Toda²,

Kobori³

et al. 2021

Preprint

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Agaricus blazei Murrill or Himematsutake is an edible and medicinal mushroom. Agaricus blazei Murrill's fruiting body extracts have anticancer properties, although the mechanism is unknown. Basic or organic solvents, which are hazardous for human health, are generally used to prepare Agaricus blazei Murrill's extracts. Inhibition of immune checkpoint molecules and Axl receptor is an effective therapy in cancer. This study assessed whether subcritical water extracts of the Agaricus blazei Murrill's fruiting body or mycelium affect the expression of Axl and immune checkpoint molecules in lung cancer cells. We used A549 cells and mouse bone marrow-derived dendritic cells in the experiments. We prepared subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium. The subcritical water extracts from the Agaricus blazei Murrill's fruiting body or mycelium significantly inhibited the expression of immune checkpoint molecules and Axl compared to saline-treated cells. Also, the hot water extract, subcritical water extract, and the hot water extraction residue subcritical water extract from the Agaricus blazei Murrill's mycelium significantly enhanced the expression of maturation markers in dendritic cells. These observations suggest that the subcritical water extract from Agaricus blazei Murrill's mycelium is a promising therapeutic tool for stimulating the immune response in cancer.

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Section: Preparation Of Abm Fruiting Body Extracts At Subcritical Conditions and Increasing Temperaturesmentioning

confidence: 69%

Section: Preparation Of Abm Fruiting Body Extracts At Subcritical Conditions and Increasing Temperaturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor

Yasuma¹,

Toda²,

Kobori³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…However, acidic conditions may cause the scission of glycosidic bonds, while alkaline conditions may cause excessively high impurities in the extract (Yi et al., 2020). The reaction conditions for enzymatic extraction are mild, and the extracts are not easily denatured (Leong et al., 2021). However, this method usually needs higher requirements for experimental conditions such as temperature and system pH values.…”

Section: Introductionmentioning

confidence: 99%

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

Meng

Chen

et al. 2021

Journal of Food Science

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The present study discussed the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily polysaccharides (DPs). The extracted crude polysaccharides were further separated and purified, and the antioxidant activities including 1,1‐diphenyl‐2‐111 picrylhydrazyl (DPPH) radical scavenging, 2,2'‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulphonate) (ABTS) radical scavenging, hydroxyl radical scavenging, and ferric‐reducing antioxidant power (FRAP) activities of the obtained fractions were also evaluated. The results showed that the optimal ultrasonic‐assisted extraction parameters with DPs yield of 15.25 ± 1.13% were water to powder ratio of 25 ml/g, extraction power of 694 W, extraction temperature of 71°C, extraction time of 38 min, and three times extraction. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions (DP‐1, DP‐2, DP‐3, and DP‐4) were successfully obtained. Monosaccharide component analysis showed that the four obtained fractions were all hetero‐polysaccharides that mainly contained rhamnose, arabinose, fructose, galactose, glucose, galacturonic acid, and glucuronic acid in different molar ratios. All the four DP fractions did show obvious antioxidant activities in vitro, and the DP‐3 component had relatively high ABTS free radical scavenging activity. Overall, our research showed that DPs could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics. Practical Application This article deals with the optimization of the ultrasonic‐assisted extraction of polysaccharides from daylily and its antioxidant activities. The results showed that the optimal ultrasonic‐assisted extraction yield of DPs was 15.25 ± 1.13%. By DEAE Sepharose Fast Flow column, four water‐soluble polysaccharide fractions were successfully obtained, and all the four DP fractions did show obvious antioxidant activities in vitro. Daylily polysaccharides could provide cheap raw materials for the development of natural antioxidants in medicines, functional foods, and even cosmetics.

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“…The contents of crude polysaccharides are influenced by the cultivars and the characteristics of the environment ( Kimmons et al, 2010 ). The extraction and purification technologies affect the yield of polysaccharides ( Leong et al, 2020 ). Specifically, the yield of crude polysaccharide extract obtained from freshly harvested fruiting bodies varies from 260 to 825 mg/100 g in fresh weight ( Monic et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes

Chen

Liu

et al. 2021

Front. Microbiol.

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Polysaccharides separated from Lentinula edodes are well known for their medicinal properties. However, the precise molecular mechanisms of polysaccharide biosynthesis in L. edodes remain unclear. In this study, the fruiting bodies of L. edodes in four developmental stages with significant differences in polysaccharide yield were collected, and the characteristics of polysaccharides were studied. De novo sequencing and comparative transcriptomic analysis were performed by using high-throughput Illumina RNA-sequencing. KS1P30, KS2P30, KS3P30, and KS4P30 were obtained from the four developmental stages, respectively, by hot water extraction and 30% ethanol precipitation. These four polysaccharides had good immune activity in vitro; all of them were β-glucopyranose with a high molecular weight. Glucose was the main monosaccharide component of these polysaccharides. High-quality clean reads (57.88, 53.17, 53.28, and 47.56 million for different growth stages) and mapping ratios ranging from 84.75 to 90.11% were obtained. In total, 11,493 (96.56%) unigenes and 18,924 (97.46%) transcripts were successfully annotated in five public databases. The biosynthetic pathway and related genes of LEFP30 were mined. The molecular mechanism of LEFP30 yield change in the different developmental stages was predicted. The results provide some insights into the possible mechanisms involved in the biosynthetic pathway of this kind of polysaccharide in L. edodes fruiting bodies. They also indicate that candidate genes can be used as important resources for biotechnology and molecular breeding to regulate L. edodes fruiting body polysaccharide biosynthesis.

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Extraction of polysaccharides from edible mushrooms: Emerging technologies and recent advances

Cited by 162 publications

References 119 publications

Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor

Subcritical Water Extracts from <em>Agaricus blazei</em> Murrill's Mycelium Inhibits the Expression of Immune Checkpoint Molecules and Axl receptor

Optimization of ultrasonic‐assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study

De novo Sequencing and Comparative Transcriptome Analyses Provide First Insights Into Polysaccharide Biosynthesis During Fruiting Body Development of Lentinula edodes

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