Extraction of Polyphenolic Compounds from Eucalyptus globulus Bark: Process Optimization and Screening for Biological Activity

Mota, Maria Inês Ferreira da; Pinto, Paula C. Rodrigues; Novo, Catarina Cardoso; Sousa, Gabriel; Guerreiro, Olinda; Guerra, Ângela R.; Duarte, Maria F.; Rodrigues, Alírio E.

doi:10.1021/ie300103z

Cited by 61 publications

(39 citation statements)

References 59 publications

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“…The ash content of bark was 1.96%, which is lower than some previously reported values for Eucalyptus globulus, e.g., 4.7% (Vázquez et al 2008) and 4.9% (Yadav et al 2002), but similar to other reported values, e.g., 2.9% (Miranda et al 2012) and 2.3% (Mota et al 2012, Pereira 1988. The wood had nearly five times less ash (0.42%) than bark, which is in agreement with published results (Queiroz 1973;Pereira and Sardinha 1984).…”

Section: Chemical Compositionsupporting

confidence: 77%

Modeling and Optimization of Eucalyptus globulus Bark and Wood Delignification using Response Surface Methodology

Neiva¹,

Gominho²,

Pereira³

2014

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Statistical models and optimization of Eucalyptus globulus Labill. wood and bark delignification were achieved using response surface methodology (RSM). A central composite design was outlined to model the simultaneous influence of active alkali (AA), reaction temperature (T), and reaction time (t) on pulp yield (η) and kappa number (k) during the kraft pulping of wood and bark. Experimental results were fitted to a second-order polynomial with linear interaction of factors. The statistical models showed high coefficients of determination for both wood (R . Using these models, the optimum conditions to attain a pulp with the highest yield at an acceptable kappa number (below 17) were determined. For wood, the pulp yield was 51.6% (51.5% predicted) and the kappa number was 16.1 (16.9 predicted) under optimized conditions (AA = 21%, T = 151 ºC, and t = 118 min). For bark, the pulp yield was 51.3% (51.0% predicted) and the kappa number was 19.4 (16.9 predicted) under optimized conditions (AA = 15%, T = 166 ºC, and t = 114 min). The degree of polymerization (DP) of the carbohydrates for the optimized pulps, 1430 and 1151 for wood and bark, respectively, suggests low levels of polysaccharide degradation. The bark delignification showed similar behavior to wood.

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Section: Chemical Compositionsupporting

confidence: 77%

Modeling and Optimization of Eucalyptus globulus Bark and Wood Delignification using Response Surface Methodology

Neiva¹,

Gominho²,

Pereira³

2014

BioResources

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“…Literature data with this regard are similar: e.g. for E. globulus 5% (Yadav et al 2002;Vázquez et al 2008), 4% (Senelwa and Sims 1999), 3% (Miranda et al 2013), and 2% (Mota et al 2012), E. grandis × E. urophylla 4% and E.grandis 7% (Lima et al 2013), E. nitens 4% and E. saligna 4% (Senelwa and Sims 1999).…”

Section: Chemical Compositionmentioning

confidence: 58%

“…Most of the bark is currently left in the forest or burnt in the mills, where the logs are debarked. In addition to its fuel value, bark could also be a source of high-value chemicals for a variety of fields, from pharmaceutical and bioactive compounds to green polymers, and bio-based materials (Conde et al 1996;Pietarinen et al 2006;Sen et al 2010;Mota et al 2012;Vázquez et al 2012;). Tree barks are seen with a renewed interest as a bioresource within the framework of green chemistry and biorefineries.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the extraction of phenolic compounds as natural antioxidants was reported for the bark of E. globulus (Conde et al 1995;Mota et al 2012;Santos et al 2012a,b;Vázquez et al 2012), E. cama ldulensis and E. rudis (Conde et al 1996;Cadahía et al 1997), E. exserta (Li and Xu 2012), E. astringens, E. clado calys, E. occidentalis, and E. sideroxylon (Fechtal and Riedl 1991). The lipophilic composition of bark was also studied, namely for E. globulus (Freire et al 2002;Domingues et al 2010), E. grandis, E. urophylla × E. grandis, and E. maidenii (Domingues et al 2011a).…”

Section: Introductionmentioning

confidence: 99%

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Chemical characterization of the bark ofEucalyptus urophyllahybrids in view of their valorization in biorefineries

et al. 2016

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Eucalyptus urophylla hybrids are important raw materials for the forest industry in Brazil, and large quantities of barks are available at mill site that may be used for added-value products. The chemical composition of barks of six commercial hybrids clones of E. urophylla × E. grandis, E. urophylla × E. Camaldulensis, and undisclosed E. urophylla hybrids was studied. The hybrids had similar composition, on average (data based on oven dry bark): 16% extractives, mainly corresponding to polar compounds that are soluble in ethanol and water, 19% lignin, 47% polysaccharides, 1% suberin, and 2% ash. The polysaccharides consists mainly of cellulose as indicated by 84% of total neutral monosaccharides in the acid hydrolysate and 10% xylose. The compositions of the lipophilic extracts was determined by GC-MS before and after alkaline hydrolysis. In all the barks, fatty acids, and triterpenes (namely: betulinic and ursolic acids) were the most abundant compounds followed by smaller amounts of sterols, long-chain aliphatic alcohols, phenolic acids, and acylglycerols. The ethanol-water bark extract had a high phenolic content: total phenolics ranged 211-551 mg gallic acid equivalents (GAE) g -1 of extract, tannins 76-184 mg catechin equivalents (CE) g -1 extract, and flavonoids 98-234 mg CE g -1 of extract. The antioxidant activity corresponds to 338 mg Trolox g -1 of extract. Development of high-value products is proposed through an integrated biorefinery approach including valorisation of extractives and targeting for cellulose-based applications.

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“…In Anatolia, Eucalyptus plants have also been used in folk medicine for antiseptic, antimicrobial, astringent, and deodorant purposes, as well as anti-fever, stimulant, antispasmodic, and haemostatic agents (Ashour, 2008;Mulyaningsih et al, 2010;Topçu et al, 2011). Eucalyptus also contains a wide variety of phenolic compounds with antioxidant, antiproliferative, anti-inflammatory, anti-hyperglycemic, and anti-thrombotic properties (Gilles et al, 2010;Mota et al, 2012;Santos et al, 2012). In recent years, the area of natural antioxidants has developed significantly due to increasing limitations on the use of synthetic antioxidants and enhanced public awareness of health issues (Vázquez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction

et al. 2015

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Eucalyptus species have found their place in traditional medicine and pharmacological research and they have also been shown to possess a large number of phenolic compounds and antioxidants. The present study sought to implement conventional extraction to yield maximal total phenolic content (TPC), total flavonoid content (TFC), proanthocyanidins, antioxidants, and saponins from E. robusta using different solvents. The most suitable extraction solvent was further employed for extracting phytochemicals from E. saligna, E. microcorys, and E. globulus to select the Eucalyptus species with the greatest bioactive compound content. The results emphasised the efficiency of water in extracting TPC ((150.60 ± 2.47) mg of gallic acid equivalents per g), TFC ((38.83 ± 0.23) mg of rutin equivalents per g), proanthocyanidins ((5.14 ± 0.77) mg of catechin equivalents per g), and antioxidants ABTS ((525.67 ± 1.99) mg of trolox equivalents (TE) per g), DPPH ((378.61 ± 4.72) mg of TE per g); CUPRAC ((607.43 ± 6.69) mg of TE per g) from E. robusta. Moreover, the aqueous extract of E. robusta had the highest TPC, TFC and antioxidant values among the other Eucalyptus species tested. These findings highlighted the efficiency of conventional extraction in extracting natural bioactive compounds from Eucalyptus species for pharmaceutical and nutraceutical applications.

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Extraction of Polyphenolic Compounds from Eucalyptus globulus Bark: Process Optimization and Screening for Biological Activity

Cited by 61 publications

References 59 publications

Modeling and Optimization of Eucalyptus globulus Bark and Wood Delignification using Response Surface Methodology

Modeling and Optimization of Eucalyptus globulus Bark and Wood Delignification using Response Surface Methodology

Chemical characterization of the bark ofEucalyptus urophyllahybrids in view of their valorization in biorefineries

Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction

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