Extraction of PLA2 and antibacterial activity test of lionfish (Pterois volitans) spine venom

Sommeng, Andy Noorsaman; Ramadhan, Muhammad Yusuf Arya; Larasati, Rizki; Ginting, Mikael Januardi; Sahlan, Muhamad; Hermansyah, Heri; Wijanarko, Anondho

doi:10.1088/1755-1315/462/1/012040

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…The major achievements of the presented study were: i. The selection and implementation of the optimum protocols through literature [17] for the isolation of venom from the lionfish's thorns ii. The selection and application of the optimum protocols through literature [18] for the isolation of tetradotoxin from the muscle tissue and organs of the L.sceleratus iii.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Utilization of Toxins from Marine Invasive Species Towards the Management of Their Population

Papadaki

Pappou

Panagiotis

et al. 2022

EJSD

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The pufferfish Lagocephalus sceleratus and lionfish Pterois miles, are two of the most important marine alien species of the Mediterranean basin. L.sceleratus is one of the most toxic species on the planet, as its tissues contain Tetradotoxin (TTX), a neuroparalytic toxin that can cause death if consumed, while lionfish is also venomous and causes a wide range of symptoms when its venom is injected through the hard thorns of its fins. Both species have rapidly developed large populations in the Eastern Mediterranean basin, while showing increasing signs of spread in the Central and Western part, causing a wide range of socioeconomic and environmental impacts. Finding innovative methods for their economic exploitation is a basic condition in order to create incentives for their targeted fishing and to achieve the limitation of their populations. A study was conducted on the isolation and identification techniques of toxins from both species. In the context of the utilization of the tetradotoxin (TTX) contained in L.sceleratus, an attempt was made to integrate it into products of high added value, such as cosmetics, after the study of its encapsulation in a polymer matrix through the electro-hydrodynamic process of electrospraying.

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Section: Discussionmentioning

confidence: 99%

Isolation and Utilization of Toxins from Marine Invasive Species Towards the Management of Their Population

Papadaki

Pappou

Panagiotis

et al. 2022

EJSD

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“…The supernatants were pooled. Sommeng et al (2020a) performed venom extraction of P. volitans from Indonesian sea waters to isolate phospholipase A2. In this experiment, briefly, they firstly immersed 50 g of the spine in 0.01 M phosphate buffer and 0.1 M CaCl2 for 8 min at 40℃.…”

Section: Extractions Of Crude Venom From Spinesmentioning

confidence: 99%

“…The authors also concluded that lionfish venom should be further studied for its possible pharmaceutical effects on Alzheimers' disease. Sommeng et al (2020a) determined that the coagulant activity of crude P. volitans spine venom can accelerate blood clot (procoagulant) up to 8.5 seconds. The LC-MS/MS analysis detected the procoagulant compound Nomega-nitro-L-arginine methyl ester (L-NAME).…”

Section: Bioactivities Of Venomous Spinesmentioning

confidence: 99%

A Review on the Potential Usage of Lionfishes (Pterois spp.) in Biomedical and Bioinspired Applications

Uyan

2022

Natural and Engineering Sciences

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Lionfishes are recognized as invasive and predatory species in the world. The genus Pterois belongs to the Scorpaenidae family has 10 confirmed species. Among them, two species Pterois miles and P. volitans were reported as non-indigenous species in Turkish marine waters. Although dorsal, pelvic, and anal fins of lionfish have venom-releasing spines which can have systemic effects such as severe pain, fever, vomiting, and dizziness, there are no reports of death by lionfish in the literature anyway. After careful removal of the venomous spines, lionfish meat is suitable for human consumption. The bioactivity of homogenates obtained from the venomous spines of lionfish has been the subject of various research since the 1950s. Until now, it has been proven that the venomous spines of the lionfish contain anticancer, antiviral, anti-inflammatory, anticoagulant, antioxidant, and antibacterial compounds and can be used in biomedical applications as a natural resource of marine origin. Moreover, the spine shape of lionfish has provided inspiration that can be useful in designing reusable syringe needles and sterilizable plungers, thereby reducing biomedical waste and sharps disposal costs. Additionally, the fins and spines of lionfish, which are typically removed and discarded, are used to make jewellery. This review, therefore, focused on evaluating alternative management actions to bring these invasive fish into the economy and control their populations, owing to both their use in biomedical fields and their suitability for bioinspiration.

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“…Activity against Gram-negative bacteria has a more significant inhibition zone than Gram-positive bacteria because Gram-negative bacteria have less peptidoglycan (Zolfagharian et al, 2016). In other microorganisms, 6.29 μg of PLA2 from Acanthaster plancii can inhibit S.aureus (Gram-positive) by 8.5 mm (Ibrahim et al, 2013) and PLA2 from Pterois volitans has antibacterial activity against S. aureus with an inhibition zone of 14.35 mm (Sommeng et al, 2020).…”

Section: Antibacterial Activity Assaymentioning

confidence: 99%

Isolation and Antibacterial Activity of Honey Bee Venom Bioactive from Apis cerana

Lischer¹,

Mustika²,

Sahlan³

et al. 2022

IJTech

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Honey bee venom from Apis species has garnered considerable attention in medicine due to its bioactive, which have antimicrobial activity. This research aims to isolate bioactive melittin and phospholipase A2 (PLA2) of honey bee venom from Indonesian Apis cerana and examine the antibacterial activity of these bioactive. Honey bee venoms were isolated from four locations using an electric shock method and purified using Fast Protein Liquid Chromatography. Purified protein was analyzed by SDS PAGE and measured by the Lowry method. Further, the antibacterial activity of melittin and PLA2 against Gram-positive (Staphylococcus aureus and Salmonella typhi) and Gramnegative (Escherichia coli) bacteria was defined by inhibition zone. Our results show that the average of all bee venoms obtained from four locations with eight sampling times was 17.1 mg proteins. Melittin was purified using gradient steps with 55%, 90%, and 100% elution buffer, while PLA2 was purified using 55% elution buffer. Melittin and PLA2 showed molecular weights of less than 7.5 kDa and 19 kDa, respectively. Protein concentrations in melittin and PLA2 were 95 µg/mL and 142 µg/mL, respectively. Among the two bioactive, only PLA2 showed antibacterial activity. The diameter of the inhibition zone was 7.76 mm when the concentration of PLA2 was 45 µg/mL against E.coli. This result showed that PLA2 from Indonesian Apis cerana has antibacterial activity and may be a promising candidate for the antibacterial agent on Gram-negative bacteria. These findings may contribute to developing new antibacterial substances derived from Indonesian Apis cerana.

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Extraction of PLA2 and antibacterial activity test of lionfish (Pterois volitans) spine venom

Cited by 3 publications

References 8 publications

Isolation and Utilization of Toxins from Marine Invasive Species Towards the Management of Their Population

Isolation and Utilization of Toxins from Marine Invasive Species Towards the Management of Their Population

A Review on the Potential Usage of Lionfishes (Pterois spp.) in Biomedical and Bioinspired Applications

Isolation and Antibacterial Activity of Honey Bee Venom Bioactive from Apis cerana

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