Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Sari, Rafael; Conterno, Paula; Silva, Letícia Dangui da; Lima, Vanderlei Aparecido de; Oldoni, Tatiane Luiza Cadorin; Thomé, Gustavo R.; Carpes, Solange Teresinha

doi:10.3390/molecules25102391

Cited by 12 publications

(7 citation statements)

References 52 publications

(88 reference statements)

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“…The differences in the antioxidant capacity in previous studies and the present investigation are highly likely due to the variation in geographical location and environmental conditions (soil and climate) of the plant sample/type of plant organ used [49][50][51]. Furthermore, according to Dutta and Ray [47], extracts often display a variation in their antioxidant potential, which is regularly associated with the usage of various base solvents and several extraction methods, that usually alters the composition of phytochemical compounds and therefore scavenges free radicals differently based on their polarity.…”

Section: Antioxidant Activitycontrasting

confidence: 55%

“…The total phenolic assay revealed a moderate number of phenols (5.89 ± 0.29 mg GAE/g) [48]. In another study, Sari et al [49] evaluated the total phenolic content of Tabernaemontana catharinensis extracts which presented a value of 23.34 mg GAE/g. However, in comparison to Boligon et al [50], significant differences were observed for the same species, T. catharinensis, which ranged from 135.57 to 562.780 mg GAE/g.…”

Section: Total Phenolic Contentmentioning

confidence: 99%

“…A previous study revealed that T. divaricata ethanolic, hexane, and ethyl acetate stem extracts exhibited weak ferric reducing antioxidant capacities when compared to butylated hydroxytoluene (BHT) [58], which are very similar to the current FRAP results. However, Sari et al [49] reported high levels of antioxidant activity in T. catharinensis using many techniques such as DPPH, FRAP, and many more. The IC 50 values for the FRAP assay were very weak (>1000 µg/mL), with all extracts besides the latex extracts (42.22 µg/mL) which showed moderate activity when compared to the control gallic acid (29.44 µg/mL) (Table 4).…”

Section: Antioxidant Activitymentioning

confidence: 99%

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In Vitro Investigation of the Antioxidant and Cytotoxic Potential of Tabernaemontana ventricosa Hochst. ex A. DC. Leaf, Stem, and Latex Extracts

Naidoo

Dewir

et al. 2022

Horticulturae

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Tabernaemontana ventricosa (Apocynaceae) a latex-bearing plant is used in traditional medicine for its therapeutic benefits in reducing fever and hypertension and wound healing. Due to limited information on the plant’s pharmacological activities, this study aimed to investigate the antioxidant potential of the leaf, stem, and latex extracts of T. ventricosa, using the Folin-Ciocalteu (total phenolics), aluminum chloride colorimetric (total flavonoids), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The cytotoxic activity was evaluated in the human HEK293 (embryonic kidney), HeLa (cervical carcinoma), and MCF-7 (breast adenocarcinoma) cell lines using the MTT assay. The latex extracts possessed the highest total phenolic content (115.36 ± 2.89 mg GAE/g), followed by the stem hexane extracts (21.33 ± 0.42 mg GAE/g), the chloroform leaf (7.89 ± 0.87 mg GAE/g), and the chloroform stem (4.69 ± 0.21 mg GAE/g) extracts. The flavonoid content was substantially high ranging from 946.92 ± 6.29 mg QE/g in the stem hexane, 768.96 ± 5.43 mg QE/g in the latex, 693.24 ± 4.12 mg QE/g in the stem chloroform, and 662.20 ± 1.00 mg QE/g in the leaf hexane extracts. The DPPH assays showed the highest percentage of inhibition at 240 µg/mL, for the stem hexane (70.10%), stem methanol (65.24%), and stem chloroform (60.26%) extracts, with their respective IC50 values of 19.26 µg/mL (stem hexane), 6.19 µg/mL (stem methanol), and 22.56 µg/mL (stem chloroform). The FRAP assays displayed minimal inhibition ranging from 4.73% to 14.40%, except for the latex extracts which displayed moderate inhibition at 15 µg/mL (21.82%) and substantial inhibition at 240 µg/mL (98.48%). The HeLa and MCF-7 cell lines were the most sensitive to the extracts, with the hexane, chloroform, and methanol leaf and stem, and latex extracts significantly affecting the percentage cell survival. Overall, the various parts of T. ventricosa exhibited strong antioxidant activity correlating to its cytotoxicity. Further studies should focus on the isolation of specific antioxidant compounds that could be investigated for their anticancer potential.

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Section: Antioxidant Activitycontrasting

confidence: 55%

Section: Total Phenolic Contentmentioning

confidence: 99%

Section: Antioxidant Activitymentioning

confidence: 99%

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In Vitro Investigation of the Antioxidant and Cytotoxic Potential of Tabernaemontana ventricosa Hochst. ex A. DC. Leaf, Stem, and Latex Extracts

Naidoo

Dewir

et al. 2022

Horticulturae

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“…Siendo los compuestos fenólicos metabolitos secundarios, producidos por esta planta, presentando propiedades biológicas como es la actividad antioxidante y la antimicrobiana (3,12,21). Donde a la actividad antioxidante de los compuestos polifenoles, se ha atribuido la capacidad de eliminar los radicales libres, responsables de causar trastornos como la diabetes, las inflamaciones, el parkinson, el Alzheimer y el cáncer (8,20,22). (4,9,11,13).…”

Section: Introductionunclassified

Efecto antibacteriano de la sangre de drago en cultivos in vitro en cepas bacterianas ATCC

Masabanda

Hidalgo

2023

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Objetivo. El objetivo de la investigación fue evaluar el efecto antibacteriano de la sangre de drago (Crotón lechleri) en cultivos in vitro de cepas bacterianas ATCC; Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922, a diferentes concentraciones 1%, 2%, 4%, 6%, 8%, 16%, 80%, 90% y 100%. Materiales y métodos. Se realizó un total 54 cultivos, de los cuales 27 cultivos corresponden al análisis de extracto Sangre de drago (Crotón lechleri) en distintas concentraciones frente a Staphylococcus aureus ATCC 25923 y 27 cultivos al análisis del extracto Sangre de drago (Crotón lechleri) frente a la bacteria Escherichia coli ATCC 25922, donde se aplicó 3 repeticiones para cada tratamiento. Resultados. En los resultados no se observó ningún halo de inhibición de los discos de sensibilidad preparados en diferentes concentraciones por lo que se consideran las cepas bacterianas ATCC; Staphylococcus aureus 25923 y Escherichia coli 25922, no presentan sensibilidad a la sangre de drago (Crotón lechleri). Conclusiones. De los resultados obtenidos podemos concluir que la sangre de drago (Crotón lechleri) no presenta actividad antibacteriana para las cepas de cepas bacterianas ATCC; Staphylococcus aureus 25923 y Escherichia coli 25922.

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“…It is a toxic substance, when in contact with skin it causes irritation and after intake is causes headaches, problems with metabolism, damages to the heart, kidneys and liver (Rudzanova et al, 2019;Solomakaou and Goula, 2021). Phenol is also damaging to water environment as it causes for example the oxidative stress in water organisms, so it is essential to prevent its penetration to surface and underground waters (Sari et al, 2020). The most popular methods for phenol removal are adsorption on e.g.…”

Section: Introductionmentioning

confidence: 99%

Membranes obtained on the basis of cellulose acetate and their use in removal of phenol from liquid phase

Przybył¹,

Bazan-Woźniak²,

Nosal‐Wiercińska³

et al. 2021

Physicochem. Probl. Miner. Process.

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The membranes based on cellulose acetate (CA) were obtained by the method of phase inversion and used for removal of phenol (in concentrations of 15 and 25 mg/L) from liquid phase. To differentiate the hydrophilic properties of the membrane surfaces, different amounts of cellulose acetate (14 and 18 wt. %) and polyvinylpyrrolidone as a pore-generating agent (PVP, 1, 2, 3 or 4 wt. %) were used. The membranes were characterised by determination of their porosity, equilibrium water content, wetting angle and content of surface oxygen functional groups. After the application of membranes to phenol removal, the following parameters characterising the process were determined: permeability, membrane resistance, coke resistances, pore resistances, total filtration resistance and flux recovery ratio. The membranes were found to show higher effectiveness in phenol removal from a solution of the initial concentration 15 mg/L, and more effective were the membranes with higher contents of cellulose acetate. On the surface of the membranes the oxygen functional groups of acidic nature are dominant, both before and after filtration. The membranes of higher contents of cellulose acetate show higher resistances.

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Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Cited by 12 publications

References 52 publications

In Vitro Investigation of the Antioxidant and Cytotoxic Potential of Tabernaemontana ventricosa Hochst. ex A. DC. Leaf, Stem, and Latex Extracts

In Vitro Investigation of the Antioxidant and Cytotoxic Potential of Tabernaemontana ventricosa Hochst. ex A. DC. Leaf, Stem, and Latex Extracts

Efecto antibacteriano de la sangre de drago en cultivos in vitro en cepas bacterianas ATCC

Membranes obtained on the basis of cellulose acetate and their use in removal of phenol from liquid phase

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