Extraction of oleuropein and luteolin-7-O-glucoside from olive leaves: Optimization of technique and operating conditions

Lama-Muñoz, Antonio; Contreras, María del Mar; Cara, Cristóbal; Moya, Manuel; Torres, Antonia de; Romero, Inmaculada; Castro, Eulógio

doi:10.1016/j.foodchem.2019.04.075

Cited by 73 publications

(53 citation statements)

References 30 publications

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“…The extraction procedure was performed in static mode using a hydroalcoholic mixture as a solvent, and all parameters (temperature, pressure, time, concentration of solvent) were optimized for enhancement of recovery yield for target compounds. The authors obtained oleuropein and luteolin-7- O -glucoside through accelerated solvent extraction (extraction method from PLE category) with an increased yield of recovery comparing to classical maceration (27.01 g/kg of dry leaf for PLE method and 63.35 g/kg of dry leaf for classical maceration for oleuropein, respectively 1.18 and 2.74 g/kg for luteolin-7- O -glucoside) [85].…”

Section: Recovery Of Antioxidants From Agro-industrial Side Streamsmentioning

confidence: 99%

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

et al. 2019

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Large amounts of agro-industrial waste are being generated each year, leading to pollution and economic loss. At the same time, these side streams are rich source of active compounds including antioxidants. Recovered compounds can be re-utilized as food additives, functional foods, nutra-/pharmaceuticals, cosmeceuticals, beauty products, and bio-packaging. Advanced extraction techniques are promising tools to recover target compounds such as antioxidants from agro-industrial side streams. Due to the disadvantages of classical extraction techniques (such as large amounts of solvents, increased time of extraction, large amounts of remaining waste after the extraction procedure, etc.), and advanced techniques emerged, in order to obtain more efficient and sustainable processes. In this review paper aspects regarding different modern extraction techniques related to recovery of antioxidant compounds from wastes generated in different industries and their applications are briefly discussed.

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Section: Recovery Of Antioxidants From Agro-industrial Side Streamsmentioning

confidence: 99%

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

et al. 2019

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“…Olive leaves are the most important by‐products obtained during harvesting and olive oil production. The annual amount of this waste in the olive industries may exceed 1 million tons, 3 which is still regarded as a residue to be used as a fuel after drying or as an animal feed. However, the chemical composition reveals good potential for use as a source of bioactive compounds, although most of these are still undervalued and wasted, resulting in other opportunities for new olive leaf‐based products that imply the transformation of waste to a source.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC‐DAD method for determination of oleuropein and other bioactive compounds in olive leaf by‐products

et al. 2020

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BACKGROUND Oil mills could benefit by preparing their own aqueous extracts from olive leaves. Accordingly, the present study aimed to measure the bioactive compounds richness of such extracts, especially oleuropein. A water‐based microwave extraction procedure was developed and a selective and precise high‐performance liquid chromatography with diode array detection (HPLC‐DAD) method was validated for the determination of oleuropein and others bioactive compounds from olive leaves. RESULTS The water solubility of oleuropein was determined to be 9.5 g L−1. The extraction procedure was optimized in terms of power, olive leaf weight/water volume ratio and time of extraction, and the results revealed that 2 mg mL−1 and a microwave irradiation at 800 W for 30 s resulted in the greatest efficiency. Oleuropein was determined by the new validation method, which showed good linearity (r2 = 0.996), precision (% relative standard deviation < 10%), recovery (118.6%), and limits of detection (17.48 mg L−1) and quantification (21.54 mg L−1). Good correlation (r2 = 0.979) was obtained between oleuropein of the olive leaf extracts determined by HPLC‐DAD and by UV‐visible spectrophotometry. CONCLUSION A simple extraction method was developed and validated to obtain aqueous extract from olive leaves by microwave extraction, determining for the first time oleuropein water solubility. Validation of the method showed that oleuropein in olive leaves could be quantified when it is at least 1% of dry weight by means of HPLC‐DAD. UV‐visible spectrophotometry can be useful in oil mills because it enables the content of oleuropein and other bioactive compounds content to be determined in situ in such leaf aqueous extracts. © 2020 Society of Chemical Industry

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“…Ethanol, methanol, and hydroalcoholic solvents are the most typical solvents used for extraction of a wide range of hydrophilic and lipophilic compounds in olive leaves. The conventional solid-liquid extraction methods, although offering some advantages (including effectiveness of maceration methods for heat-susceptible compounds, and the accuracy/reproducibility of Soxhlet methods), represent the downsides of (i) inefficient diffusivity and a slow/prolonged extraction process [103,104], (ii) using a prodigious amount of organic solvents (with potential toxicity), (iii) lengthy extraction time, and (iv) poor processing adaptability [6]. Other disadvantages inherent in both thermal and non-thermal conventional extractions are the high energy use, high production cost, and the potential incidence of degradation, arising from (i) prolonged subjection to solvents, e.g., in maceration [104], and (ii) the use of a high temperature involved in thermal operation [6,103].…”

Section: Conventional Extraction Techniquesmentioning

confidence: 99%

“…Many attempts have been made to develop modified/advanced technologies for the efficient extraction of bioactive compounds from olive leaves [6,104]. In the literature, a range of emerging techniques have been proposed to date as alternative tools to replace traditional/inefficient processing systems.…”

Section: Alternative Strategies To Improve Extraction Performancementioning

confidence: 99%

Olive Tree Leaves—A Source of Valuable Active Compounds

2020

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The agricultural and processing activities of olive crops generate a substantial amount of food by-products, particularly olive leaves, which are mostly underexploited, representing a significant threat to the environment. Olive leaves are endowed with endogenous bioactive compounds. Their beneficial/health-promoting potential, together with environmental protection and circular economy, merit their exploitation to recover and reuse natural components that are potentially safer alternatives to synthetic counterparts. These biomass residues have great potential for extended industrial applications in food/dietary systems but have had limited commercial uses so far. In this regard, many researchers have endeavoured to determine a green/sustainable means to replace the conventional/inefficient methods currently used. This is not an easy task as a sustainable bio-processing approach entails careful designing to maximise the liberation of compounds with minimum use of (i) processing time, (ii) toxic solvent (iii) fossil fuel energy, and (iv) overall cost. Thus, it is necessary to device viable strategies to (i) optimise the extraction of valuable biomolecules from olive leaves and enable their conversion into high added-value products, and (ii) minimise generation of agro-industrial waste streams. This review provides an insight to the principal bioactive components naturally present in olive leaves, and an overview of the existing/proposed methods associated with their analysis, extraction, applications, and stability.

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Extraction of oleuropein and luteolin-7-O-glucoside from olive leaves: Optimization of technique and operating conditions

Cited by 73 publications

References 30 publications

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

Recovery of Natural Antioxidants from Agro-Industrial Side Streams through Advanced Extraction Techniques

Development and validation of an HPLC‐DAD method for determination of oleuropein and other bioactive compounds in olive leaf by‐products

Olive Tree Leaves—A Source of Valuable Active Compounds

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