Extraction of Oil from Chlorella vulgaris Using Supercritical Carbon Dioxide and Organic Solvent

Abstract

Three different types of extraction processes, which used supercritical carbon dioxide (SCCO 2 ) and organic solvent, were attempted to improve the extraction yield of oil from Chlorella vulgaris: cosolvent-modified SCCO 2 extraction, SCCO 2 extraction with ultrasonic sample treatment in organic solvent, and static extraction with organic solvent followed by dynamic SCCO 2 extraction. Among these, the last SCCO 2 extraction process was found to be most effective in the extraction of oil. Compared with pure SCCO 2 extraction, the extraction yield of oil was observed to increase about 7 times.