Extraction of Nobiletin and Tangeretin From Peels of Shekwasha and Ponkan Using [C₂mim][(MeO)(H)PO₂] and Centrifugation

Abstract

The polymethoxyflavonoids nobiletin and tangeretin are isolated from peels of shekwasha (Citrus depressa Hayata) and ponkan (Citrus reticulata Blanco). These natural products possess biological activities, including anticancer and anti-inflammation properties. This report describes an efficient method for the extraction and isolation of nobiletin and tangeretin from citrus peels using 1-ethyl-3-methylimidazolium methylphosphonate ([C 2 mim][(MeO)(H)PO 2 ]) and centrifugation/decantation. The results showed that the extraction yield of nobiletin and tangeretin using [C 2 mim][(MeO)(H)PO 2 ]/organic solvent (1:1 w/w) was 1.4 to 1.6 times higher than that using organic solvent. This extraction method is expected to be applicable for obtaining other organic compounds from natural sources.

A Brief Review of Natural Products with Urate Transporter 1 Inhibition for the Treatment of Hyperuricemia

“…Protein docking analysis revealed that baicalein interacted with Ser35 and Phe241 of URAT1 [41]. Nobiletin is a highly methoxylated flavone compound, especially abundant in citrus [40]. Cell experiments showed that nobiletin (IC 50 � 17.6 μM/l) could inhibit URAT1 expression and uric acid uptake in 293A cells expressing URAT1 treated with uric acid [51].…”