Extraction of native collagen from limed bovine split wastes through improved pretreatment methods

Li, Dong; Yang, Wei; Li, Guoying

doi:10.1002/jctb.1912

Cited by 24 publications

(13 citation statements)

References 28 publications

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“…1 However, as a natural polymer, collagen had some weakness in its properties, including the relatively lower thermal stability and a high vicidity, which are limiting factors for its application. The denaturation temperature (a temperature at which a transition of collagen from a rod-like molecule to a randomly coiled form occurs) 2 of collagen in solution was no more than 39 C and the complex viscosity of collagen with concentration of 1.5% reached up to 406.2 Pa s (25 C), 3,4 which means that collagen was easy to denature or was difficult to flow in pipe during processing. For improving the properties of collagen, blending of collagen with other polymers has been of a considerable interest in recent years.…”

Section: Introductionmentioning

confidence: 99%

Rheological properties of collagen/hydroxypropyl methylcellulose (COL/HPMC) blended solutions

Ding

Zhang

2013

J of Applied Polymer Sci

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Collagen/HPMC blends with different HPMC content (HC) were investigated by dynamic responses, creep recovery, thixotropy, and morphological observation. Storage modulus, loss modulus, complex viscosity, and activation energy decreased with the increased HC, while the flow behavior index increased with the increased HC and the dynamic denaturation temperature reached the maximum as HC increased to 50%, indicating that the flowability and thermal stability of collagen solution were improved by the addition of HPMC. However, the blends with higher HC tended to have a relatively lower recovery capacity, and the hysteresis loop areas of the blends were lower than that of collagen (especially when HC > 50%). Additionally, the morphology of collagen/ HPMC was examined by both of atomic force microscopy and scanning electron microscopy. By combining with these results, it seemed that the rheological and structural properties of collagen/HPMC were related to the hydrogen-bond interaction and compatibility between collagen and HPMC molecules.

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Section: Introductionmentioning

confidence: 99%

Rheological properties of collagen/hydroxypropyl methylcellulose (COL/HPMC) blended solutions

Ding

Zhang

2013

J of Applied Polymer Sci

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“…At present, the main sources of collagen are skin or bone of domestic animals such as cows or pigs. Different techniques have been proposed to obtain collagens from these sources, generally in neutral salts10 or acidic solutions 11, 12. However, due to the outbreaks of foot and mouth disease, bovine spongiform encephalopathy, and transmissible spongiform encephalopathy in cattle and pigs, the use of collagen and collagen‐derived products from these sources has been limited 13.…”

Section: Introductionmentioning

confidence: 99%

Human collagen isolated from adipose tissue

Kim

Choi

Kim

et al. 2012

Biotechnology Progress

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Collagen, the most abundant protein in vertebrates, is a useful biomaterial in pharmaceutical and medical industries. So far, most collagen has been extracted from animals and cadavers. Herein, we suggest human adipose tissue, which is routinely abandoned after liposuction, as a plentiful source of human collagen. In this study, human collagen was obtained from adipose tissue through two successive major steps: (i) extraction of the extracellular matrix (ECM) by pulverization, centrifugation, alkaline, and alcohol treatment; (ii) isolation of collagen from ECM by pepsin treatment in dilute acetic acid. The purified human adipose-derived collagen was characterized by Fourier transform infrared spectroscopy, polyacrylamide gel electrophoresis, amino acid analysis, and circular dichroism spectroscopy. The extracted collagen showed a typical triple helix structure, good thermal stability due to abundant imino acids, and high solubility at acidic pH. The collagen greatly facilitated the adhesion and proliferation of human adipose-derived stem cells and normal human dermal fibroblasts on polystyrene plates. These results suggest that human adipose tissue obtained by liposuction can provide human collagen for use in cosmetics, pharmaceutics, and medicine.

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“…The maximum transition temperature (T m ) of collagen was determined by DSC200PC (Netzsch, Germany). The endothermal curve of the sample was recorded from 20 to 50 • C at a scanning rate of 3 • C/min in a nitrogen atmosphere [19].…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 99%