Extraction of lipase from Rhodotorula glutinis fermentation culture by aqueous two-phase partitioning

Khayati, Gholam Reza; Alizadeh, Sahebeh

doi:10.1016/j.fluid.2013.05.037

Cited by 32 publications

(25 citation statements)

References 16 publications

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“…Results were evaluated by a few parameters including: protein partition coefficient in the aqueous two-phase systems was defined as [8]:…”

Section: Methodsmentioning

confidence: 99%

“…It has been demonstrated that the molecular weight of PEG affects its distribution in the two phases and polymer-protein interactions [8]. Therefore, in order to select a suitable PEG molecular weight for the extraction of b-galactosidase from A.…”

Section: Selection Of Polymer Molecular Weightmentioning

confidence: 99%

“…These systems can form a gentle environment for enzymes and other biologically active proteins, therefore, the extraction in the systems has the advantages of high capacity, high activity yields and easy to scale up. These systems have many applications for protein purification in biochemistry and biotechnology [8,9]. The partitioning of biomolecules in ATPS is influenced by pH, temperature, surface properties, size and concentrations of the biomolecules and the types of employed polymers and salts [10].…”

Section: Introductionmentioning

confidence: 99%

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Partitioning of β-galactosidase in aqueous two-phase systems containing polyethyleneglycol and phosphate salts

Khayati

Anvari

Shahidi

2015

Fluid Phase Equilibria

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“…Results were evaluated by a few parameters including: protein partition coefficient in the aqueous two-phase systems was defined as [8]:…”

Section: Methodsmentioning

confidence: 99%

Section: Selection Of Polymer Molecular Weightmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Partitioning of β-galactosidase in aqueous two-phase systems containing polyethyleneglycol and phosphate salts

Khayati

Anvari

Shahidi

2015

Fluid Phase Equilibria

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“…This is because the type of additives and/or impurities and their concentration in the aqueous solution directly affect both the system features and the solute properties. [13] For the efficacy of aqueous twophase extraction (ATPE), it is very important to select a suitable phase system. In ATPSs, the most significant and difficult step is to choose the appropriate type and composition of the system in terms of achieving efficient extraction of the biomolecule.…”

Section: Resultsmentioning

confidence: 99%

Extraction of lipase fromRhizopus microsporusfermentation culture by aqueous two-phase partitioning

Anvari

2015

Biotechnology & Biotechnological Equipment

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The purification of a desired protein from the complex mixture of biomolecules in a fermentation broth is one of the main challenges in industrial-scale biotechnological processes. For partial purification of industrial enzymes, one possible approach is to use aqueous two-phase extraction (APTE) as a primary downstream processing step. In the present study, the feasibility of utilizing ATPE for the purification of lipase from Rhizopus microsporus fermentation culture was investigated. The effects of the phase composition and salt type, molecular weight of the polyethylene glycol (PEG), pH of the system, sample loading and addition of neutral salts on lipase partitioning were investigated at 24 C. In most of the examined aqueous two-phase systems (ATPSs), lipase showed affinity to the top phase. Optimum conditions for lipase purification were obtained in 20% PEG 2000/12% (NH 4 ) 2 SO 4 , with 5% Na 2 CO 3 addition at pH D 8 for 30% crude load. For the optimized ATPS, the recovery yield and purification factor in the top phase were determined to be 92.3% and 19.8, respectively.

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“…Aqueous two-phase systems (ATPSs) are promising liquid-liquid extraction systems for biomaterial and metal separation because they are primarily composed of water and other components which are neither toxic nor flammable (Khayati, 2013;Khayati and Anvari, 2012;Khayati and Alizadeh, 2013;Lacerda et al, 2009;Patricio et al, 2011). ATPSs are formed when two mutually incompatible hydrophilic solutes are dissolved in water above a certain critical concentration (Hatti-Kaul, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effective Extraction of Zn(II) Ions Using Aqueous Two-Phase Partitioning

Khayati

Mohamadian

2014

Chemical Engineering Communications

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This study evaluates the influence of various process parameters such as type of aqueous two-phase systems, phase forming salt, molecular weight of the phase forming polymer, phase composition, phase volume ratio, type of neutral salts and temperature on the extraction yield of Zn (II) ions to determine the best conditions for the recovery of Zn (II) ions in aqueous two-phase systems (ATPS) composed of by poly(ethylene glycol) (PEG) and a salt (sodium sulphate, ammonium sulphate, potassium citrate and sodium citrate).In all the systems, Zn (II) ions presented affinity for the PEG-rich phase. The 31.36% PEG 2000 + 7.45% sodium citrate system with NaCl showed the highest values of extraction yield. In addition, thermodynamic parameters (ΔH• t , ΔS• t , ΔG• t ) as a function of temperature, were calculated for this system and the results implied that the partitioning of Zn (II) ions was controlled by entropy changes.

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Extraction of lipase from Rhodotorula glutinis fermentation culture by aqueous two-phase partitioning

Cited by 32 publications

References 16 publications

Partitioning of β-galactosidase in aqueous two-phase systems containing polyethyleneglycol and phosphate salts

Partitioning of β-galactosidase in aqueous two-phase systems containing polyethyleneglycol and phosphate salts

Extraction of lipase fromRhizopus microsporusfermentation culture by aqueous two-phase partitioning

Effective Extraction of Zn(II) Ions Using Aqueous Two-Phase Partitioning

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