Extraction of forskolin from Coleus forskohlii roots using three phase partitioning

Harde, Shirish M.; Singhal, Rekha S.

doi:10.1016/j.seppur.2012.05.017

Cited by 53 publications

(13 citation statements)

References 29 publications

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“…It is also possible to observe that a higher content of carbohydrate is found in the control compared to the enzymatically treated samples. This might be due to hydrolysed carbohydrate partitioned into the aqueous phase (polar phase) as explained by Harde and Singhal (2012). Most importantly, it was observed that protein concentration in the concentrate increased after enzymatic treatment.…”

Section: Effect Of Enzymatic Treatment On Protein Concentrationmentioning

confidence: 95%

“…Previous reports indicate that TPP can be significantly affected by enzymatic treatment (Harde and Singhal 2012). In this study two enzymes were used: (1) Stargen ™ 002 is enzyme mixture that contains Aspergillus kawachi alpha-amylase expressed in Trichoderma reesei and a glucoamylase from Trichoderma reesei that work synergistically to hydrolyse starch to glucose at optimum conditions (pH 4.5, temperature 50 °C) and (2) Carezyme ™ which is cellulase from Aspergillus sp.…”

Section: Effect Of Enzymatic Treatment On Protein Concentrationmentioning

confidence: 99%

“…Three-phase partitioning (TPP) is a separation technique useful for initial purification of proteins with high recovery yields. It has gained a lot of interest due to its rapid, simple and scalable use for concentration, isolation and decontamination of proteins from crude samples (Harde and Singhal 2012). TPP can efficiently fractionate nonpolar components in solvent phase (upper phase), polar components in aqueous (lower phase) and proteins in the middle phase.…”

Section: Introductionmentioning

confidence: 99%

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Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Waghmare

Salve

LeBlanc

et al. 2016

Bioresour. Bioprocess.

172

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Background: Many methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very time-consuming. Three-phase partitioning (TPP) is a separation technique that has gained a lot of interest due to its rapid, simple and scalable use for concentration, isolation and decontamination of proteins from crude samples with high recovery yields. In the present work, the effect of various parameters of TPP was evaluated to optimize the concentration of proteins from Chlorella pyrenoidosa (CP), is green algae that increasingly being used as food supplements because of its positive impacts on human health. Results:Chlorella pyrenoidosa was cultivated in a closed system under controlled conditions. After reaching maximum growth, the microalgae was harvested, dried and powdered. Afterwards, TPP of CP cell lysate was done to concentrate protein content. To maximize protein concentration, various parameters were optimized such as solvent (t-butanol), ammonium sulphate concentration (40 % w/v), solid load (0.75 g/20 mL), pH (6), incubation time (20 min), slurry to butanol ratio (1:1.5) and enzymatic treatment (combination of Stargen ™ and Carezyme ™ ). Also, total starch, cellulose and carbohydrate content before and after the enzymatic treatment were determined to comprehend the impact of enzymatic treatment on protein concentration. Using these optimized parameters, 78.1 % w/w protein concentration was obtained in middle protein concentrate phase. This protein concentrate was characterizedfor proximate composition, colour analysis, water holding capacity, oil-holding capacity, foaming capacity, foam stability, amino acid composition, protein quality and thermal properties. Conclusion:Various process parameters of TPP influence the protein concentration of middle protein concentrate phase. Enzymatically treated biomass also enhanced protein concentration in middle protein concentrate phase. Characterization of protein concentrate revealed the presence high-quality protein. Therefore, it is possible to implement TPP at an industrial scale for protein concentration.

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Section: Effect Of Enzymatic Treatment On Protein Concentrationmentioning

confidence: 95%

Section: Effect Of Enzymatic Treatment On Protein Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Waghmare

Salve

LeBlanc

et al. 2016

Bioresour. Bioprocess.

172

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show abstract

“…In the TPP system, t-butanol as cosolvent has been reported to possess a number of advantages over other organic solvents (methanol and ethanol), e.g., higher molecular size, branched chain structure, signicant crowding, and kosmotropic effects at a temperature range of 20-30 C. 4,32 In general, t-butanol increases the buoyancy of the precipitated protein by binding to the protein, which causes the protein to oat above the lower aqueous phase. 33 Furthermore, t-butanol has been commonly used in studies on TPP of proteins and PS.…”

Section: Effect Of T-butanol To Crude Extract Ratiomentioning

confidence: 99%

Extraction and characterization of anti-oxidative polysaccharide–protein complexes from Corbicula fluminea through three-phase partitioning

et al. 2017

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In this study, a simple, efficient, and green bioseparation technology called three-phase partitioning (TPP) was employed to extract and separate polysaccharide-protein complexes (PSP) from Corbicula fluminea.The highest extraction yield of PSP was 9.0% under the following optimal conditions: 20% (w/v) ammonium sulfate concentration, 1.5 : 1.0 (v/v) t-butanol to crude extract ratio, 30 min, and 35 C. This value was higher than the yield (7.6%) of E-PSP prepared through conventional enzyme extraction. The carbohydrate content (81.7%) of PSP was higher and its molecular weight (61.5 kDa; 72.4%) was lower than those of E-PSP.Although PSP and E-PSP are proteoglycans with different monosaccharide and amino acid compositions, their primary chemical structures did not remarkably differ after TPP was performed. The antioxidant activities of PSP were more evident than those of E-PSP in vitro. Thus, TPP could be potentially used for separation of active PS or PSP from natural sources.

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“…Three-phase partitioning is fundamentally a combination of techniques called salting out, co-solvent precipitation, osmotic and cosmotropic effects. Unique advantages of three-phase partitioning might be specified as simple, inexpensive, versatile, highly efficient technique and it is easily convertible into pilot scale [6].…”

mentioning

confidence: 99%

Three-Phase Partitioning of Peroxidase from Flowers of Citrus sinensis for Use in Diagnostic Kit

Uckaya¹,

Demir²

2018

Asian J. Chem.

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Extraction of forskolin from Coleus forskohlii roots using three phase partitioning

Cited by 53 publications

References 29 publications

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Extraction and characterization of anti-oxidative polysaccharide–protein complexes from Corbicula fluminea through three-phase partitioning

Three-Phase Partitioning of Peroxidase from Flowers of Citrus sinensis for Use in Diagnostic Kit

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