Extraction of extracellular polymeric substances (EPS) from a newly isolated self-flocculating microalga Neocystis mucosa SX with different methods

Lv, Junping; Zhao, Fei; Feng, Jia; Liu, Qi; Nan, Fangru; Xie, Shulian

doi:10.1016/j.algal.2019.101479

Cited by 36 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After recovery, it is important to pretreat the biomass to obtain EPS bound to microalgal cells [12,60]. Researchers describe that up to 50% of the total EPS can remain bound to the cell of these microorganisms.…”

Section: Recent Advances In Harvesting Algae and Pretreatments For Th...mentioning

confidence: 99%

“…However, there are no standard methods for this extraction. The use of chemical reagents such as formaldehyde, ethylenediaminetetraacetic acid, sodium hydroxide, as well as sonication, heating, and washing with distilled wa-ter and/or complexation/treatment with ionic resins, were performed to recover these polysaccharides from the surfaces of microalgal cells [12,60,61].…”

Section: Recent Advances In Harvesting Algae and Pretreatments For Th...mentioning

confidence: 99%

See 1 more Smart Citation

Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants

et al. 2022

View full text Add to dashboard Cite

Microalgae are used in flocculation processes because biopolymers are released into the culture medium. Microalgal cell growth under specific conditions (temperature, pH, luminosity, nutrients, and salinity) provides the production and release of exopolysaccharides (EPS). These biopolymers can be recovered from the medium for application as bioflocculants or used directly in cultivation as microalgae autoflocculants. The optimization of nutritional parameters, the control of process conditions, and the possibility of scaling up allow the production and industrial application of microalgal EPS. Therefore, this review addresses the potential use of EPS produced by microalgae in bioflocculation. The recovery, determination, and quantification techniques for these biopolymers are also addressed. Moreover, other technological applications of EPS are highlighted.

show abstract

Section: Recent Advances In Harvesting Algae and Pretreatments For Th...mentioning

confidence: 99%

Section: Recent Advances In Harvesting Algae and Pretreatments For Th...mentioning

confidence: 99%

Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Cation exchange resin (CER) [108,109], ethylene diamine tetraacetic acid (EDTA) [110,111], and sodium tripolyphosphate (STPP) [112] have been applied as chemical agents to extract both EPS and cellular enzymes from AS. The mechanism of enzyme extraction is similar for all of these chemical agents and involves removing multivalent cations from sludge EPS to disrupt AS flocs.…”

Section: Cation Removal Agentsmentioning

confidence: 99%

Enzyme Recovery from Biological Wastewater Treatment

Liu¹,

Smith²

2020

Waste Biomass Valor

View full text Add to dashboard Cite

Enzymes are high value industrial bio-catalysts with extensive applications in a wide range of manufacturing and processing sectors, including the agricultural, food and household care industries. The catalytic efficiency of enzymes can be several orders higher compared to inorganic chemical catalysts under mild conditions. However, the nutrient medium necessary for biomass culture represents a significant cost to industrial enzyme production. Activated sludge (AS) is a waste product of biological wastewater treatment and consists of microbial biomass that degrades organic matter by producing substantial quantities of hydrolytic enzymes. Therefore, enzyme recovery from AS offers an alternative, potentially viable approach to industrial enzyme production. Enzyme extraction from disrupted AS flocs is technically feasible and has been demonstrated at experimental-scale. A critical review of disruption techniques identified sonication as potentially the most effective and suitable method for enzyme extraction, which can be scaled up and is a familiar technology to the water industry. The yields of different enzymes are influenced by wastewater treatment conditions, and particularly the composition, and can also be controlled by feeding sludge with specific target substrates. Nevertheless, hydrolytic enzymes can be effectively extracted directly from waste AS without specific modifications to standard wastewater treatment processes. Purification, concentration and stabilisation/immobilisation techniques can also greatly expand the industrial application and increase the economic value and marketability of enzyme products extracted from AS. Concentrated and purified AS enzymes could readily substitute inorganic and/or commercial bioenzyme catalysts in many industrial applications including, for example, leather processing, and in detergent and animal feed formulation. Enzyme extraction from AS therefore offers significant economic benefits to the Water Industry by recovering valuable resources from wastewater. They can also optimise important waste treatment processes, such as the anaerobic digestion (AD) of sewage sludge, increasing biogas and renewable energy production. The enzyme-extracted sludge exhibits improved treatment properties, such as increased settleability, dewaterability, and anaerobic digestibility for biogas production, assisting sludge management by wastewater treatment plants (WWTPs) and enabling the further utilisation of the residual sludge. Graphic Abstract

show abstract

“…Several studies indicate that polyethylene could be degraded by photo-degradation and chemical degradation methods (Genovese et al, 2016;Peixoto et al, 2017) In addition, biodegradation of polyethylene occurs by two mechanisms: hydro-biodegradation and oxo-biodegradation (Singh and Sharma, 2008). Microorganisms can easily attach to the surface of hydrophilic polyethylene, with the matrix being hydro-biodegraded (Lv et al, 2019). The usage of enzymes and microbes for the bioremediation of plastics can still break down the larger polymeric molecules into simple forms (Saengsawang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Due to the availability of nutrients, water, and sunlight, microalgal colonies have been found to be dominating on the surface of the discarded polyethylene bags (Cunha et al, 2019). As a result of the available literature, it has been established that microalgae can colonise the surface of polyethylene sheets and that biodegradation occurs under normal conditions (Cunha et al, 2019;Lv et al, 2019). A microalgae consortium was used for a recent study showing some positive hetero aggregation on the polymers.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Microalgae’s Plastic Biodeterioration Property by a Consortium of Chlorella sp. and Cyanobacteria sp.

Bhuyar

Sundararaju

Feng

et al. 2021

EREM

View full text Add to dashboard Cite

Malaysia is one of the top eight countries that has a drawback of mismanaged plastic waste. This study intended to investigate polymer degradation using the biological technique with the help of microalgae to minimise the time required for biodegradation. This research article aims to identify the collected sample with the most suitable microalgae for the biodegradation of microplastic and to analyse the biodegradation of the polymer by microalgae. The results revealed that the consortium of Chlorella sp. and Cyanobacteria sp. were able to deteriorate low-density polyethene (LDPE sample) through several stages, and this was confirmed by UV-Spec, FESEM, EDX, CHNO, FTIR and DSC analysis. The results obtained revealed that microalgae producing exopolysaccharides (EPS) decreased the carbon and oxygen ratio. According to SEM micrographs, microalga may colonise, agglomerate, and adhere microplastics to its surface, regardless of its fractional size. The EDX analysis showed that the initial composition of carbon was 92.30 ± 1.23 %, while after the incubation, the carbon composition started decreasing from 53.18 % to 39.12 ± 1.08 %. Finally, there was a 37.91 % decrease in carbon weight from elemental analysise

show abstract

Extraction of extracellular polymeric substances (EPS) from a newly isolated self-flocculating microalga Neocystis mucosa SX with different methods

Cited by 36 publications

References 42 publications

Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants

Recent Advances of Microalgae Exopolysaccharides for Application as Bioflocculants

Enzyme Recovery from Biological Wastewater Treatment

Evaluation of Microalgae’s Plastic Biodeterioration Property by a Consortium of Chlorella sp. and Cyanobacteria sp.

Contact Info

Product

Resources

About