Extraction of digoxin and its metabolites from urine and their separation by sephadex LH-20 column chromatography

Gault, M. H.; Ahmed, Mohamed; Symes, Abbey; Vance, J.

doi:10.1016/s0009-9120(76)80013-6

Cited by 17 publications

(2 citation statements)

References 9 publications

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“…Most reports have indicated that the combined figure is ordinarily <8% _ 2, 7, 9. 10, 11,18 However, Clark and Kalman,5 using the combination of thin layer, column, and gas chromatography, found that up to 42% of ingested digoxin was excreted as glycosidic metabolites. Results in this study for the urinary excretion of digoxigenin and its mono-and bis-digitoxosides are roughly comparable to those found in earlier reports, except for that of Clark and Kalman,5 and support the view that of the glycosidic metabolites, the bis-digitoxoside of digoxigenin is ordinarily present in the largest quantity.…”

Section: Discussionmentioning

confidence: 99%

Biotransformation and elimination of digoxin with normal and minimal renal function

Gault

Sugden

Maloney

et al. 1979

Clin Pharma and Therapeutics

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Six subjects with normal renal jimction (NRF) and 6 patients with minimal renal function (MRF) on 3 times weekly hemodialysis received 150 jJ-Ci :lH-digoxin-120' orally. Serial urine collections were made for jive days or more. Digoxin and metabolites were separated using diethylaminoethyl Sephadex LH-20 column chromatography. Mean cumulative percentages of the ingested radioactivity excreted over jive days in NRF and MRF groups were: digoxin, 54.5% and 14.7%; bis-digilOxoside of digoxigenin, 2.0% and 0.50%; mono-digitoxoside, 0.8% and 0.19%; digoxigenin, 0.25% and 0.03%; and dihydrodigoxin, 0.3% and 0.03%. Haif~lives based on the mean rates of disappearance from urine comparing NRF and MRF groups were: for digoxin, 40 hr and 120 hr; for bis-digitoxoside, 11.5 hr and 46 hr;for mono-digitoxoside, 8.5 hr and 12 hr; for digoxigenin, 2 hr and 7.5 hr; and for dihydrodigoxin, 1.2 hr and 7.0 hr. Considering the relationships of the jive-day cumulative excretion and half-lives of digoxin and metabolites in the NRF and MRF groups, it appears unlikely that there is a major alteration in the biotransformation of digoxin in advanced renal failure It'hen There appears to be a shijt from renal to slower hiliary excretion.

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Section: Discussionmentioning

confidence: 99%

Biotransformation and elimination of digoxin with normal and minimal renal function

Gault

Sugden

Maloney

et al. 1979

Clin Pharma and Therapeutics

Self Cite

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show abstract

“…Whether there is an important accumulation of digoxin metab olites in blood under these conditions has not been established. This could be important because the digoxin radioimmunoassay clearly measures metab olites to varying degrees [23], and the mono and bis digitoxosides, digoxigenin and the dihydro derivatives have differing amounts of cardiac activity and toxicities [24,40,54], However, a preliminary study of digoxin metabolites separated using Sephadex LH20 (Pharmacia Fine Chemicals, Uppsala, Swe den) column chromatography from urines collected serially from 5 patients with advanced renal failure does not suggest appreciable accumulation [68]. Also, the good therapeutic response in patients with advanced renal failure and mean SDCs of 0.8 and 1.8 ng/mi (table la, b, II), docs not suggest that an increase in metabolites will limit the use of the nomogram.…”

Section: Absorption Metabolism and Excretionmentioning

confidence: 99%