Extraction of cholesterol from cattle brain using supercritical carbon dioxide

Optimization of process parameters for supercritical fluid extraction of cholesterol from whole milk powder using ethanol as co‐solvent

“…Generally, in a binary extraction system, the solubility of cholesterol increases with extraction temperature, which shows that a higher vapor pressure of solute at elevated temperature has a more pronounced effect than the density of scCO 2 . 27,42 In the present study, an opposite effect was observed, indicating a negative influence of temperature on the solute-co-solvent interaction. In a ternary solvent system (solute-ethanol-scCO 2 ), co-solvent affects the crossover of solubility isotherms in scCO 2 , leading to the unusual reversal of co-solvent effects.…”

“…The hydrogen bonding capacity of ethanol/isopropyl alcohol with cholesterol is higher compared to that of acetone. 26,27 Randolph et al 28 observed that co-solvents have a prominent effect on the aggregation of cholesterol in scCO 2 , where ethanol promotes a more compact arrangement than n-butanol and methanol. When 1.5% (w/w) ethanol was added as a co-solvent in SCFE, the solubility of lipids increased by 50% over the pure CO 2 value.…”

Selective fractionation of cholesterol from whole milk powder: optimisation of supercritical process conditions

“…Increased temperatures resulted in marginal increase in the recovery throughout the pressure ranges studied. Higher vapour pressure of SC-CO 2 at elevated temperature increases the penetration ability into the biological matrix and hence improves extractability by improving the diffusion coefficient (Mohamed et al, 2000;Vedaraman et al, 2004). While modelling the solubility of cholesterol and its esters in SC-CO 2 in a binary system, Huang et al (2004) found that solubility increased with the increase in temperature from 313.15 to 323.15 K in the pressure regions of 150-300 bar.…”

Determination of cholesterol in food samples using dispersive liquid–liquid microextraction followed by HPLC–UV

“…There is no sufficient evidences support the link between the disease and dietary cholesterol but it can be oxide to some harmful by-product when exposed to the air [2][3][4].…”