Extraction of Bioactive Components from Chamaenerion angustifolium (L.) Scop. with Choline Chloride and Organic Acids Natural Deep Eutectic Solvents

Abstract

Chamaenerion angustifolium (L.) Scop. (fireweed) is a perennial herbaceous plant of the Onagraceae family widely used in folk and scientific medicine. It is a promising source of bioactive components. One of the modern trends in extraction is the use of natural deep eutectic solvents (NADESs) combined with ultrasound-assisted extraction (UAE). However, works devoted to the extraction of biologically active substances from C. angustifolium using NADESs are scarce. The aim of this work is a comprehensive study of UAE of bioactive components from C. angustifolium using NADESs based on choline chloride and malonic, malic, tartaric, and citric acids. The antioxidative properties, total phenols, and flavonoids content were estimated for NADES-based extracts. The reference solvents were water and 90% v/v ethanol. Volatile extracted components were identified using GC-MS. The kinetics of the UAE were studied at 45 °C for 20–180 min with water added to 30 wt% NADES. The power of the ultrasound was 120 W, and the frequency was 40 kHz. It was found that NADES choline chloride + citric acid is more effective for the extraction of bioactive components. For this, NADES UAE conditions were optimized following a Box–Behnken design of the experiment and a response surface methodology. The temperature ranged from 30 to 60 °C, the time of extraction ranged from 20 to 60, and the addition of water ranged from 30 to 70 wt%. We established the optimal extraction conditions: temperature 58 °C, time of extraction 35 min, and 70 wt% water. The obtained results expand the knowledge about the use of NADES for the extraction of biologically active compounds from cheap and available plant raw materials.

Efficient Extraction and Purification of Isofraxidin from Acanthopanax Senticosus by DES

“…Indeed, previous studies have used the above-mentioned DES to extract other active ingredients, but it is worth noting that almost all previous studies have synthesized DES and used them directly to extract active ingredients or for other purposes. For example, Tsvetov et al [24] used choline chloride and L-ascorbic acid to synthesize DES to extract bioactive components from chamaenerion angustifolium, Luo et al [25] used choline chloride and L-ascorbic acid to synthesize DES to extract secondary metabolites from eucommia ulmoides leaves, and Zhang et al [26] used glycerol and levulinic acid to synthesize DES extracting flavonoids from Acanthopanax senticosus, other functions include the synthesis of DES removal of copper corrosion products using choline chloride and L-ascorbic acid by Akiko Tsurumaki et al [27]. However, there are a few studies that have performed short-term storage of newly synthesized DES, for example, Andrew J Maneffa et al [28] used choline chloride and other compounds to synthesize DES.…”

Extraction of phenolic compounds from spent coffee ground using natural deep eutectic solvents: New modeling approach

“…These models have the advantage of being simple, of requiring less experimental data for regression of kinetic parameters, of provide optimal time of the process and can be used to compare the effectiveness of extraction with traditional organic solvents (Huang et al, 2022; Tsvetov et al, 2022). However, as shortcomings, these models need to know the value of the concentration at equilibrium for each operating condition $$$ \left({C}_{eq}\right) $$$ to provide information on extraction behavior over time, but the other operating conditions (e.g., temperature, water content, etc.)…”

Application of Natural Deep Eutectic Solvents for Extraction of Bioactive Components from Rhodiola rosea (L.)

“…The chemical analysis of the extracts was carried out in accordance with the methods described in the works [ 24 , 25 ]. Briefly, total polyphenols content (TPC) was evaluated using the Folin–Ciocalteu method.…”

“…The antiradical activity was evaluated by the DPPH method. Both procedures were described in detail in works [ 24 , 25 ]. The extract was diluted 400 times in ethanol for the DPPH method.…”