Extraction of Aucubin from Seeds of Eucommia ulmoides Oliv. Using Supercritical Carbon Dioxide

Li, Hui; Hu, Jiangyu; Ouyang, Hongwei; Li, Yanan; Shi, Hui; Cheng-jin, MA; Zhang, Yongkang

doi:10.1093/jaoac/92.1.103

Cited by 13 publications

(6 citation statements)

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“…We found that E. ulmoides Oliver significantly regulated hepatic lipid accumulation both in vitro and in vivo . Our study results suggest that the regulatory mechanism of E. ulmoides Oliver and its active constituents, aucubin and geniposide [22] , [23] , toward hepatic dyslipidemia involves regulation of ER stress and associated lysosomal activity.…”

Section: Introductionmentioning

confidence: 65%

Eucommia ulmoides Oliver Extract, Aucubin, and Geniposide Enhance Lysosomal Activity to Regulate ER Stress and Hepatic Lipid Accumulation

Lee

et al. 2013

PLoS ONE

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Eucommia ulmoides Oliver is a natural product widely used as a dietary supplement and medicinal plant. Here, we examined the potential regulatory effects of Eucommia ulmoides Oliver extracts (EUE) on hepatic dyslipidemia and its related mechanisms by in vitro and in vivo studies. EUE and its two active constituents, aucubin and geniposide, inhibited palmitate-induced endoplasmic reticulum (ER) stress, reducing hepatic lipid accumulation through secretion of apolipoprotein B and associated triglycerides and cholesterol in human HepG2 hepatocytes. To determine how EUE diminishes the ER stress response, lysosomal and proteasomal protein degradation activities were analyzed. Although proteasomal activity was not affected, lysosomal enzyme activities including V-ATPase were significantly increased by EUE as well as aucubin and geniposide in HepG2 cells. Treatment with the V-ATPase inhibitor, bafilomycin, reversed the inhibition of ER stress, secretion of apolipoprotein B, and hepatic lipid accumulation induced by EUE or its component, aucubin or geniposide. In addition, EUE was determined to regulate hepatic dyslipidemia by enhancing lysosomal activity and to regulate ER stress in rats fed a high-fat diet. Together, these results suggest that EUE and its active components enhance lysosomal activity, resulting in decreased ER stress and hepatic dyslipidemia.

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Section: Introductionmentioning

confidence: 65%

Eucommia ulmoides Oliver Extract, Aucubin, and Geniposide Enhance Lysosomal Activity to Regulate ER Stress and Hepatic Lipid Accumulation

Lee

et al. 2013

PLoS ONE

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“…The palmitate-induced ROS accumulation was diminished by EUE or its active components, aucubin and geniposide ( Figure S3 ). Because the palmitate-induced cell death mechanism includes the lysosomal pathway and EUE regulates cell death through lysosomal activation [24] , we examined the effect of the active components, aucubin and geniposide, on lysosomal activation. First, lysosomal V-ATPase activity was analyzed through the acridine quenching method.…”

Section: Resultsmentioning

confidence: 99%

Eucommia ulmoides Cortex, Geniposide and Aucubin Regulate Lipotoxicity through the Inhibition of Lysosomal BAX

Lee

et al. 2014

PLoS ONE

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In this study we examined the inhibition of hepatic dyslipidemia by Eucommia ulmoides extract (EUE). Using a screening assay for BAX inhibition we determined that EUE regulates BAX-induced cell death. Among various cell death stimuli tested EUE regulated palmitate-induced cell death, which involves lysosomal BAX translocation. EUE rescued palmitate-induced inhibition of lysosomal V-ATPase, α-galactosidase, α-mannosidase, and acid phosphatase, and this effect was reversed by bafilomycin, a lysosomal V-ATPase inhibitor. The active components of EUE, aucubin and geniposide, showed similar inhibition of palmitate-induced cell death to that of EUE through enhancement of lysosome activity. Consistent with these in vitro findings, EUE inhibited the dyslipidemic condition in a high-fat diet animal model by regulating the lysosomal localization of BAX. This study demonstrates that EUE regulates lipotoxicity through a novel mechanism of enhanced lysosomal activity leading to the regulation of lysosomal BAX activation and cell death. Our findings further indicate that geniposide and aucubin, active components of EUE, may be therapeutic candidates for non-alcoholic fatty liver disease.

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“…Aucubin is also a kind of iridoid compound, which is very unstable and sensitive to temperature. Therefore, longer heating process destructs the structure and decreases the content of aucubin (Li et al, 2009;Zheng et al, 2012). Table 1 reveals the lower aucubin content of all the processed samples than that of the control.…”

Section: Comparison Of Methodsmentioning

confidence: 99%

Effects of pre-processing on the active compounds before drying Eucommia ulmoides leaves

et al. 2023

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Aiming to provide a new way that preserves more active components in Eucommia ulmoides (EU) leaves, this paper utilizes 4 methods, i.e., microwave, steam, air heating, and frying, to process EU leaves before drying, and their respective effects on the leaves were evaluated. The antioxidant activity of EU leaves in vitro was measured by the DPPH free radical method; the contents of total flavonoids (TFC) and total phenols (TPC) were analyzed by spectrophotometry; and the contents of main active components were determined by high-performance liquid chromatography (HPLC). The results showed the pre-process improved the quality of the EU leaves. As a simple and fast process, the microwave process is the most effective technique in the retention of flavonoids and four active ingredients except aucubin. High power (800 W) and short time (2.5 min) are more conducive to the retention of active ingredients, with high antioxidant activity. Appropriate steam and frying processes also exhibited benefits for the active components. In particular, the samples treated with steam for 10 minutes showed great potential for industrial application with the highest antioxidant activity and polyphenol content. Therefore, proper pre-processing before drying should be conducted to effectively protect the active components of EU leaves.

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Extraction of Aucubin from Seeds of Eucommia ulmoides Oliv. Using Supercritical Carbon Dioxide

Cited by 13 publications

References 0 publications

Eucommia ulmoides Oliver Extract, Aucubin, and Geniposide Enhance Lysosomal Activity to Regulate ER Stress and Hepatic Lipid Accumulation

Eucommia ulmoides Oliver Extract, Aucubin, and Geniposide Enhance Lysosomal Activity to Regulate ER Stress and Hepatic Lipid Accumulation

Eucommia ulmoides Cortex, Geniposide and Aucubin Regulate Lipotoxicity through the Inhibition of Lysosomal BAX

Effects of pre-processing on the active compounds before drying Eucommia ulmoides leaves

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