Extraction of Arctium Lappa leaves using supercritical CO2 + ethanol: Kinetics, chemical composition, and bioactivity assessments

Souza, Ariádine Reder Custódio de; Guedes, Amanda Roman; Rodriguez, João Manoel Folador; Bombardelli, Michele Cristiane Mesomo; Corazza, Marcos L.

doi:10.1016/j.supflu.2018.06.011

Cited by 41 publications

(52 citation statements)

References 65 publications

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Section: Methodsmentioning

confidence: 99%

“…Raw material of aerial parts of A. lappa (leaves) were obtained as described in a previous study . Four extracts from A. lappa leaves (E1, E2, E3 and E3) were obtained through supercritical process using scCO 2 as solvent and ethanol (or not) as co‐solvent under different pressures (150, 200 and 250 bar) and temperatures (60 °C and 80 °C) that preserved the biological activities of the compounds . Considering that ethanol increases the solubility of polar compounds, we evaluated the presence and absence of ethanol as co‐solvent, fixing the pressure at 200 bar and temperature at 60 °C as well as the influence of pressure and temperature changes on the biological effect of A. lappa extracts on lectin pathway activation.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction conditions, the extraction yield and nomenclature of extracts are shown in Table . In addition, the extracts were previously analyzed by gas chromatography‐mass spectrometry (Agilent 7890A and Agilent 5975C), and the phytochemical compounds profile were identified ( Table ).…”

Section: Methodsmentioning

confidence: 99%

“…Different letters mean significant differences obtained by the Tukey test ( p <0.05). Adapted from Souza et al …”

Section: Methodsmentioning

confidence: 99%

“…Popularly known as Burdock, the plant Arctium lappa L. presents several biological activities including antioxidant, anti‐inflammatory, anti‐allergic, antimicrobial (bacteria and yeasts), antitumor, hypolipidemic, antidiabetic and antiulcer . Recently, Fontana et al .…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Bavia

Fontana

Bovo

et al. 2019

Chemistry & Biodiversity

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The complement system participates in host defense by eliminating microorganisms and triggering inflammation. However, insufficient control or exacerbated complement activation contributes to inflammatory diseases. Since promising antioxidant and anti-inflammatory activities have been identified in Arctium lappa L. extracts, this study aims to explore the effect of A. lappa extracts on the lectin pathway (LP) of complement activation. Four extracts were obtained by supercritical extraction using scCO 2 with or without ethanol as cosolvent, at different temperatures and pressures (E1: 2.2 mg/mL, E2: 2.6 mg/mL and E3: 2.0 mg/mL, E4: 1.5 mg/ mL). To evaluate the effect of A. lappa extracts on the LP activation, an ELISA assay using mannose binding lectin pathway of complement was carried out with C4 detection. All extracts showed a concentration-dependent inhibitory effect on the activation of complement by the LP. The following IC 50 were observed for E1, E2, E3 and E4: 179.4 μg/mL, 74.69 μg/mL, 119.1 μg/mL and 72.19 μg/mL, respectively. Our results suggest that A. lappa extracts are potential candidates for the treatment of inflammatory disorders that are complement-related. ResultsThe A. lappa extracts E1, E2, E3 and E4 displayed an inhibitory effect on the functional activity of lectin pathway (Figure 1). The extract E1 (at concentration Figure 1. Effect of Arctium lappa L. extracts on functional activity of the MBL lectin pathway of complement system. The activation of the lectin pathway was evaluated in the presence of four extracts obtained under different conditions: The extracts E1 and E2 were obtained using scCO 2 in the presence (A) and absence of ethanol (B) as co-solvent, respectively, at 200 bar and 60°C. The extract E3 was obtained using scCO 2 + ethanol at 150 bar and 80°C (C). The extract E4 was obtained using scCO 2 + ethanol at 250 bar and 80°C (D). The final concentration of all extracts evaluated is represented in the x axis. All extracts dilutions were compared with the positive control (NHS diluted to represent 50 % of lectin pathway activation) by one-way ANOVA and Bonferroni's multiple comparison test (***p < 0.001, **p < 0.01, and *p < 0.05). All biological experiments were performed twice in duplicate and the results were expressed as mean and standard deviation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Different letters mean significant differences obtained by the Tukey test ( p <0.05). Adapted from Souza et al …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Bavia

Fontana

Bovo

et al. 2019

Chemistry & Biodiversity

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Supercritical Fluid Extraction of Essential Oils from Natural Sources

et al. 2023

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Phytochemicals and Biological Activities of Burdock (Arctium lappa L.) Extracts: A Review

Souza¹,

Oliveira

Fontana

et al. 2022

Chemistry & Biodiversity

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Arctium lappa L., also known as burdock, is an edible wild plant which has the ability to grow in distinct environments and is considered a weed in several parts of the world. This species has great value in the biological and medical fields with its major secondary components being phenolic compounds and terpenes, substances rich in desired biological activities as antioxidant, antimicrobial, antitumor and anti‐inflammatory. In addition, burdock leaves extracts have shown a modulatory effect on the complement system, which plays an important role in the development of inflammatory diseases, with an inhibitory effect on all complement pathways. Thus, natural products with those relevant activities are promising agents for healthcare applications. Therefore, the species A. lappa may represent an interesting asset for researching and developing new therapies for inflammatory afflictions.

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Extraction of Arctium Lappa leaves using supercritical CO2 + ethanol: Kinetics, chemical composition, and bioactivity assessments

Cited by 41 publications

References 65 publications

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Supercritical Fluid Extraction of Essential Oils from Natural Sources

Phytochemicals and Biological Activities of Burdock (Arctium lappa L.) Extracts: A Review

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Extraction of Arctium Lappa leaves using supercritical CO2 + ethanol: Kinetics, chemical composition, and bioactivity assessments

Cited by 41 publications

References 65 publications

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Inhibitory Effect of Supercritical Extracts from Arctium lappa L. on the Lectin Pathway of the Complement System

Supercritical Fluid Extraction of Essential Oils from Natural Sources

Phytochemicals and Biological Activities of Burdock (Arctium lappa L.) Extracts: A Review

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Product

Resources

About

Extraction of Arctium Lappa leaves using supercritical CO2 + ethanol: Kinetics, chemical composition, and bioactivity assessments