Extraction of antioxidant and antiproliferative ingredients from fruits of Rubus chingii Hu by active tracking guidance

Abstract

Hu, namely "Fu-pen-zi" in Chinese, has been used as a functional food in China for a long time. This study aims to identify its bioactive constituents with antioxidant and anti-tumor properties. was extracted with 95% ethanol and then partitioned into four fractions: petroleum ether fraction, ethyl acetate fraction,-butanol fraction, and water fraction. Results showed that the ethyl acetate fraction had the strongest antioxidant activity and cytotoxicity against human cancer cell lines (HepG-2, Bel-7402, A549 and MCF-7). Therefore, four compounds were isolated from the ethyl acetate fraction, and they were identified as -16α,17-dihydroxy-kauran-19-oic acid, tormentic acid, oleanolic acid and β-daucosterol, the first two of which were isolated and identified from for the first time. In particular, tormentic acid exhibited excellent cytotoxicity activities against human tumor cell lines. The results obtained in this work might contribute to the understanding of biological activities of and further investigation on its potential application is valued for food and drugs.

Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro

“…In this work, all fractions, except E-PE, exhibited antioxidant activity in a dose-dependent manner within concentrations of 1.25-100 μg/mL. The E-EA fraction had the best antioxidant capacity in the five fractions of Rubus chingii Hu., which was in agreement with previous literature [14]. The antiaging properties of plants are attributed to contained antioxidant substances, such as polyphenols, flavonoids and anthocyanins [5].…”

“…Rubus chingii Hu. has been reported to contain flavonoids with significant antioxidant and protective effects against disease induced by oxidative stress [12][13][14]. Furthermore, Rubus chingii Hu.…”

Caucasian endemic medicinal and nutraceutical plants: in-vitro antioxidant and cytotoxic activities and bioactive compounds

“…A number of Rubus species showed cytotoxic activity. For example, the fruits of Rubus chingii Hu showed cytotoxic activity against HepG2, Bel‐7402, A549 and MCF7 human cancer cell lines, while R. idaeus L. revealed higher cytotoxic activity towards the human leukaemia cell lines: J45 and HL60 . In the current study, R. takhtadjanii , like R. chingii, showed similar cytotoxic activity against HepG2 cell lines.…”

Biological assays and theoretical density functional theory calculations of Rh(I), Ir(III), and Ru(II) complexes of chiral phosphinite ligand

“…Compounds having reducing power can be used as primary and secondary antioxidants to reduce the oxidative intermediates in the lipid peroxidation process as the electron donor. [ 55 ] As shown in Figure 2, all test samples exhibited reducing power activity. The results showed that the maximum activities of 6 , 4 , 3 , and 5 were 0.402, 0.251, 0.223, and 0.207 at a concentration of 200 mg/L, respectively.…”