Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae)

“…Three solvent systems of differing polarities were used (Kotzé and Eloff, 2002), namely ethyl acetate:methanol:water (40:5.4:5, EMW, polar, neutral), chloroform:ethyl acetate:formic acid (5:4:1, CEF, intermediate polarity, acidic) and benzene:ethanol:ammonium hydroxide (90:10:1, BEA, non-polar, basic). The extracts were analysed using TLC immediately after preparation, and again before use in the anticoccidial screening.…”

Section: Plant Collection and Preparationmentioning

confidence: 99%

The value of plant extracts with antioxidant activity in attenuating coccidiosis in broiler chickens

Naidoo

McGaw

Bisschop

et al. 2008

Veterinary Parasitology

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Coccidiosis remains one of the most important diseases in the poultry industry and results in the annual loss of millions of US dollars by the poultry industry. In South Africa and other developing countries where a large percentage of the population is unemployed, cheap food production is necessary. If the control of the coccidian parasite could be made more economical, these savings could be passed on to the consumer. In Europe, where the economics are different, people are becoming more aware of the potential dangers of using antimicrobials in producing animal protein. A solution to both these problems could be the use of plant products that function by mechanisms other than those of chemotherapeutics, with the additional advantage of a natural origin. Antioxidant compounds could hold promise for the control of Eimeria infections due to the association of coccidial infection with lipid peroxidation of the intestinal mucosa. Four plant extracts with antioxidant activity were screened for their anticoccidial activity in vivo with toltrazuril as the positive control. Combretum woodii (160 mg/kg) proved to be extremely toxic to the birds, while treatment with Tulbaghia violacea (35 mg/kg), Vitis vinifera (75 mg/kg) and Artemisia afra (150 mg/kg) resulted in feed conversion ratios similar to toltrazuril, and higher than the untreated control. T. violacea also significantly decreased the oocyst production in the birds. From this study we conclude that antioxidant-rich plant extracts have potential benefits in treating coccidial infections. The promising results obtained with T. violacea justify further studies on the potential value of the plant as a therapeutic or prophylactic anticoccidial agent.

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“…To evaluate the number of antioxidant compounds present in an extract, 10 ml of 10 mg/ml of each plant extract was spotted on a TLC plate and eluted in a polar solvent comprising the mixture of ethyl acetate, methanol and water in the ratio of 10:1.35:1 (Kotze and Eloff, 2002). The developed plate was dried and immediately sprayed with 0.2% DPPH in methanol (Sigma).…”

Section: Methodsmentioning

confidence: 99%

Can MTT be used to quantify the antioxidant activity of plant extracts?

2009

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Antioxidant properties of three different medicinal plant extracts from northern Nigeria were evaluated using the traditional qualitative 2,2, diphenyl-picrylhydrazyl (DPPH) method and a new 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) microdilution method described here. The results were in agreement. It is a simple, rapid and inexpensive method compared with many other methods for determining quantitative antioxidant activity of plant extracts.

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Extraction of antibacterial compounds from Combretum microphyllum (Combretaceae)

Cited by 124 publications

References 11 publications

In vitro inhibition of Plasmodium falciparum early and late stage gametocyte viability by extracts from eight traditionally used South African plant species

In vitro inhibition of Plasmodium falciparum early and late stage gametocyte viability by extracts from eight traditionally used South African plant species

The value of plant extracts with antioxidant activity in attenuating coccidiosis in broiler chickens

Can MTT be used to quantify the antioxidant activity of plant extracts?

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