“…Three solvent systems of differing polarities were used (Kotzé and Eloff, 2002), namely ethyl acetate:methanol:water (40:5.4:5, EMW, polar, neutral), chloroform:ethyl acetate:formic acid (5:4:1, CEF, intermediate polarity, acidic) and benzene:ethanol:ammonium hydroxide (90:10:1, BEA, non-polar, basic). The extracts were analysed using TLC immediately after preparation, and again before use in the anticoccidial screening.…”