Extraction of Amino Acids with ABS

Zafarani-Moattar, Mohammed Taghi; Hamzehzadeh, Sholeh

doi:10.1007/978-3-662-52875-4_5

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…As these bases are known kosmotropic salts, the norbornene sodium salt 9:Na , was suspected of being strongly chaotropic and the two phase system was a result of high concentrations of a kosmotrope and chaotrope. 50 – 52 To confirm this theory ammonium sulphate (a kosmotropic salt) was added to a concentrated aqueous solution of 9:Na and the rapid formation of a biphasic system validated these suspicions.…”

Section: Resultsmentioning

confidence: 99%

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Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

et al. 2018

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Section: Resultsmentioning

confidence: 99%

“…Aqueous biphasic systems have demonstrated utility in delicate separations such as those involving aliphatic carboxylic acids. 47 – 50 The biphasic systems also formed when sat. K 2 CO 3 , 5 M NaOH and 5 M KOH solutions were used.…”

Section: Resultsmentioning

confidence: 99%

Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

et al. 2018

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“…Indeed, the mechanisms governing the partitioning of amino acids in ABS containing ILs as adjuvants are more complex and are not fully understood. Nevertheless, the partitioning behavior of Phe in the studied ABS may be attributed to the resultant of the well‐known driving forces governing the partitioning of amino acids in the PEG‐ or IL‐based ABS: the phase hydrophobicity effect, the salting‐out effect, and the IL‐amino acid specific interactions such as π⋯π stacking …”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, the partitioning behavior of Phe in the studied ABS may be attributed to the resultant of the well-known driving forces governing the partitioning of amino acids in the PEG-or IL-based ABS: the phase hydrophobicity effect, the salting-out effect, and the IL-amino acid specific interactions such as pÁ Á Áp stacking. [67][68][69][70]…”

Section: Effect Of [C 4 C 1 Im]br On the Partition Coefficients Of Lpmentioning

confidence: 99%

The role of ionic liquid [C₄C₁im]Br as an adjuvant on the two‐phase formation and the extraction of l‐phenylalanine in ABS composed of PEG400 and potassium citrate at different temperatures

Hamzehzadeh

Touri

2018

Biotechnology Progress

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The potential of {polyethylene glycol 400 + potassium citrate} aqueous biphasic system (ABS) with ionic liquid (IL) 1-butyl-3-methylimidazolium bromide ([C C im]Br) as an adjuvant is examined for the extraction of l-phenylalanine (Phe), as a model biomolecule, at different temperatures and system compositions. The binodal curves and liquid-liquid equilibrium data were determined by the addition of 5 wt% IL to investigate its effect on phase diagrams and Phe partition coefficients. The results indicate that binodal curves of systems with and without IL are more deviated from each other with decreasing temperature. Moreover, IL has a high tendency to partition into the PEG-rich phase. This tendency increases with increasing temperature and system compositions. For Phe, the partition coefficients obtained in this work (K ≈ 5.5-81.2) are significantly higher than those observed in other conventional PEG-inorganic salt ABS (K ≈ 0.5-2.5), water-immiscible ILs two-phase extraction systems (K ≈ 0.02-1.2), or even, in the IL-based ABS with the same IL as the main phase-forming component (K ≈ 3.2). The phase hydrophobicity, salting-out and π⋯π stacking seem to be the main driving forces to affect the extraction aptitude of the studied ABS for Phe. Furthermore, the performance of using [C C im]Br as adjuvant to improve the partition of Phe in the studied ABS at different temperatures seems to be ruled by the differences in the phases hydrophobicities. Finally, the experimental tie lines and partition coefficients are accurately correlated using the NRTL model. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018 © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1149-1166, 2018.

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Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

Santos

Santos-Ebinuma

Pessoa

et al. 2017

J of Chemical Tech & Biotech

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Biopharmaceuticals are one of the most important groups of biotechnological products, representing one-quarter of all pharmaceutical sales and providing suitable and efficient medical care for many previously untreatable diseases. However, their production and purification usually require complex processes, resulting in extremely expensive final products. Thus, the development of newer, simpler and cheaper methods for biosynthesis and purification of these biocompounds, as well as proper integration between these stages, are crucial to reduce their commercial costs and to increase the scale of biopharmaceuticals' medical use. One solution for this concern relies on the proper integration between upstream and downstream processing applying liquid-liquid extraction systems as an intermediate stage. Recently, several works reported that a proper choice of liquid-liquid systems such as aqueous biphasic systems (ABS) can be a suitable platform to simplify, reduce the number of the downstream stages, improve the recovery and purification yields and, consequently, decrease biopharmaceuticals' manufacturing costs. This review will explain the general concept of biopharmaceuticals, their main production and purification methods, particularly, exploring the use of ABS as effective and integrative platforms for their recovery and purification, and providing further insights into the future trends and prospects in the field.

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Extraction of Amino Acids with ABS

Cited by 3 publications

References 33 publications

Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

The role of ionic liquid [C₄C₁im]Br as an adjuvant on the two‐phase formation and the extraction of l‐phenylalanine in ABS composed of PEG400 and potassium citrate at different temperatures

Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

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Extraction of Amino Acids with ABS

Cited by 3 publications

References 33 publications

Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

Norbornene chaotropic salts as low molecular mass ionic organogelators (LMIOGs)

The role of ionic liquid [C4C1im]Br as an adjuvant on the two‐phase formation and the extraction of l‐phenylalanine in ABS composed of PEG400 and potassium citrate at different temperatures

Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

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The role of ionic liquid [C₄C₁im]Br as an adjuvant on the two‐phase formation and the extraction of l‐phenylalanine in ABS composed of PEG400 and potassium citrate at different temperatures