Extraction of Acetoin From Fermentation Broth Using an Acetone/Phosphate Aqueous Two-Phase System

Sun, Jianan; Ben, Rao; Zhang, Liaoyuan; Shen, Yang; Wei, Dongzhi

doi:10.1080/00986445.2012.683901

Cited by 40 publications

(13 citation statements)

References 24 publications

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“…In addition, acetoin (3-hydroxybutan-2-one) is widely used in food, cigarettes, flavour, cosmetics, detergents, and chemical synthesis [ 39 ]. The absence of 2,3-butanediol facilitates the recovery of acetoin, which can be easily performed using an acetone/phosphate aqueous two-phase system [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Hmidet

Ayed

Jacques

2017

BioMed Research International

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This work concerns the study of the enhancement of surfactin and fengycin production by B. mojavensis A21 and application of the produced product in diesel biodegradation. The influences of the culture medium and cells immobilization were studied. The highest lipopeptides production was achieved after 72 hours of incubation in a culture medium containing 30 g/L glucose as carbon source and a combination of yeast extract (1 g/L) and glutamic acid (5 g/L) as nitrogen sources with initial pH 7.0 at 30°C and 90% volumetric aeration. The study of primary metabolites production showed mainly the production of acetoin, with a maximum production after 24 h of strain growth. The use of immobilized cells seemed to be a promising method for improving lipopeptides productivity. In fact, the synthesis of both lipopeptides, mainly fengycin, was greatly enhanced by the immobilization of A21 cells. An increase of diesel degradation capacity of approximately 20, 27, and 40% in the presence of 0.5, 1, and 2 g/L of produced lipopeptides, respectively, was observed. Considering these properties, B. mojavensis A21 strain producing a lipopeptide mixture, containing both surfactin and fengycin, may be considered as a potential candidate for future use in bioremediation and crop protection.

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Section: Resultsmentioning

confidence: 99%

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Hmidet

Ayed

Jacques

2017

BioMed Research International

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“…2 Acetoin is naturally produced in microbial fermentation processes and it has been suggested that production of acetoin and the closely related 2,3-butanediol help to prevent acidification of the cytoplasm during excessive organic acid production. 5 Biosynthesis of acetoin has been achieved in many species lacking native enzymes by heterologous overexpression of genes coding for acetolactate synthase (alsS) and acetolactate decarboxylase (alsD) from native acetoin producers, f. ex. Bacillus subtilis.…”

Section: Introductionmentioning

confidence: 99%

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

et al. 2020

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“…For fermentative products, SOE is more favorable than repulsive extraction because the concentration of the target product in the fermentation broth is too low to salt out. Recently, SOE, which is also referred to as aqueous two‐phase extraction when the extractant is a hydrophilic solvent, was widely used to separate bio‐based chemicals from the fermentation broths, for example, 2,3‐BD , acetoin , 1,3‐PD , and lactic acid .…”

Section: Introductionmentioning

confidence: 99%

Separation of bio‐based chemicals from fermentation broths by salting‐out extraction

Dai

Sun

Xiu

2014

Engineering in Life Sciences

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The possibility of creating a biorefinery using inexpensive biomass has attracted a great deal of attention, which is mainly focused on the improvement of strains and fermentation, whereas few resources have been spent on downstream processing. Bio‐based chemical downstream processing can become a bottleneck in industrial production because so many impurities are introduced into the fermentation broth. This review introduces a technique referred to as salting‐out extraction, which is based on the partition difference between chemicals in two phases consisting of salts and polymers or hydrophilic solvents, hydrophobic solvents, and amphipathic chemicals. The effects of solvents and salts on the formation of two phases were discussed, as was the use of this method to recover bio‐based chemicals. This review focused on the separation of hydrophilic chemicals (1,3‐propanediol, 2,3‐butanediol, acetoin, and lactic acid) from fermentation broths. Diols could be recovered at a high yield from fermentation broths without pretreatment especially with a hydrophilic solvent‐based system, whereas the recovery of organic acids was slightly lower. Most of the impurities (cells and proteins) were removed during the same step. Extractive fermentations were also used for polymer‐based aqueous two‐phase systems.

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Extraction of Acetoin From Fermentation Broth Using an Acetone/Phosphate Aqueous Two-Phase System

Cited by 40 publications

References 24 publications

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Enhancement of Surfactin and Fengycin Production byBacillus mojavensisA21: Application for Diesel Biodegradation

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

Separation of bio‐based chemicals from fermentation broths by salting‐out extraction

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