Extraction methods of <i>Amaranthus</i> sp. grain oil isolation

Krulj, Jelena; Brlek, Tea; Pezo, Lato; Brkljača, Jovana; Popović, Sanja; Zeković, Zoran; Solarov, Marija Bodroža

doi:10.1002/jsfa.7540

Cited by 35 publications

(42 citation statements)

References 50 publications

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“…Generally, ScCO 2 is a recommended technique for nonpolar compound extraction with molecular weights less than 500 g/mol soluble in CO 2 , such as SQ. e extraction time reduces considerably; Krulj et al [90] found differences between the solvent and ScCO 2 method using petroleum ether for the squalene extraction from three amaranth genotypes.…”

Section: Methods Of Extraction Of Squalenementioning

confidence: 99%

Plant Sources, Extraction Methods, and Uses of Squalene

Lozano-Grande

Gorinstein

Espitia-Rangel

et al. 2018

International Journal of Agronomy

175

122

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Squalene (SQ) is a natural compound, a precursor of various hormones in animals and sterols in plants. It is considered a molecule with pharmacological, cosmetic, and nutritional potential. Scientific research has shown that SQ reduces skin damage by UV radiation, LDL levels, and cholesterol in the blood, prevents the suffering of cardiovascular diseases, and has antitumor and anticancer effects against ovarian, breast, lung, and colon cancer. e inclusion of SQ in the human diet is recommended without causing health risks; however, its intake is low due to the lack of natural sources of SQ and efficient extraction methods which limit its commercialization. Biotechnological advances have developed synthetic techniques to produce SQ; nevertheless, yields achieved are not sufficient for global demand for industrial or food supplement purposes. e effect on the human body is one of the scientific issues still to be addressed; few research studies have been developed with SQ from seed or vegetable sources to use it in the food sector even though squalene was discovered more than half a century ago. e aim of this review is to provide an overview of SQ to establish research focus with special reference to plant sources, extraction methods, and uses.

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Section: Methods Of Extraction Of Squalenementioning

confidence: 99%

Plant Sources, Extraction Methods, and Uses of Squalene

Lozano-Grande

Gorinstein

Espitia-Rangel

et al. 2018

International Journal of Agronomy

175

122

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“…The variables are presented by vectors in factor space, while the correlation between two variables is indicated by the angle between vectors (the high correlation corresponds to small angle between vectors) (Krulj et al, 2016). Having this in mind, it can be observed that high positive correlation exists between AME and AOH content (r = 0.990, p < 0.001), and also the strong negative correlation to trade quality parameters VW and TKW.…”

Section: Principal Component Analysis (Pca)mentioning

confidence: 99%

Impact of Alternaria spp. and Alternaria Toxins on Quality of Spelt Wheat

Đisalov¹,

Bodroža‐Solarov²,

Krulj³

et al. 2018

JAS

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There is an increasing consumer demand for alternative cereals nowadays. Spelt wheat (Triticum aestivum ssp. Spelta) appears to be a future source for the agriculture and food sector. Alternaria spp. infections might become a serious danger to the worldwide grain industry, resulting in yield losses and reduction of end-use quality, with potential harmful effect of Alternaria toxins on human and animal health. This paper presents the first assessment of the impact of Alternaria infection and its toxins to quality of spelt wheat. The results showed that fungal contamination significantly reduced both trade and technological quality parameters. Volume weight, thousand kernel weight and wet gluten were significantly decreased, while protein content was significantly higher in Alternaria inoculated treatments. Although with slight decrease, falling number was not significantly affected by fungal contamination. The most negative impact of alternariol (AOH) was registered on volume weight and thousand kernel weight (-0.847; -0.898), while highly significant positive correlation was found between AOH and protein content (0.758). Alternaria spp. additionally destroyed spelt gluten structure, resulting in reduction of dough energy and baking quality with no significant influence of mycotoxins (AOH and AME) on technological quality parameters.

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“…The literature has compared three techniques, expeller pressing, organic solvent extraction, and supercritical fluid extraction (SCFE) showing that the best yield is achieved 69.5 g/kg by SCFE [18]. Other methods had been proved by Krulj et al [19] who extracted amaranth oil from raw seeds using accelerated solvent extraction and achieved higher yield than supercritical fluid extraction 78.1 and 61.1 g/kg, respectively. Relatively low temperature extraction results in almost no-thermal degradation of labile compounds.…”

Section: Journal Of Food Qualitymentioning

confidence: 99%

“…As mentioned above, the most common presentation to commercialize amaranth is a puffed seeds; milling seeds would limit its commercialization, increasing steps in extraction process and rising production cost; however, it is possible to obtain oily extracts from nonmilled raw seeds, although with relatively low yields [19,22].…”

Section: Journal Of Food Qualitymentioning

confidence: 99%

“…Squalene content in Soxhlet oily extracts was 44.2 ± 2.27 and 31.3 ± 1.0 squalene g/kg of oily extract from whole raw and puffed seeds respectively, whereas SCFE extraction from whole puffed seeds yielded 460 ± 28.1 g/kg squalene in the oily extract. Krulj et al [19] obtained an average oily extract yield of 58.4 g/kg and 3.5 g/kg of squalene by applying SCFE in milled raw seeds. Our results indicate that SCFE extracts had a higher squalene content than Soxhlet-extracts when puffed seeds are used, suggesting that puffing not only gives better physical, functional, and mechanical properties [36] but also makes more available oil and particularly squalene for extraction.…”

Section: Amaranth Oily Extract a Hypochondriacus Oily Extractsmentioning

confidence: 99%

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Squalene Extraction by Supercritical Fluids from Traditionally Puffed Amaranthus hypochondriacus Seeds

Rosales-García

Jiménez‐Martínez

Cardador‐Martínez

et al. 2017

Journal of Food Quality

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Extraction of squalene, a potent natural antioxidant, from puffed A. hypochondriacus seeds was performed by supercritical fluid extraction (SCFE); besides, to have a blank for comparison, extraction was performed also by Soxhlet method using organic solvents (hexane). Chemical proximal composition and seed morphology were determined in raw, puffed, and SCFE-extracted seeds. Extracts were obtained with a 500 mL capacity commercial supercritical extractor and performed between 10 and 30 MPa at 313, 323, and 333 K under constant CO 2 flow of 0.18 kg CO 2 /h during 8 h. The squalene content was determined and the fatty acids present in the extracts were identified by GC-MS. The extract obtained by SCFE from puffed amaranth seeds reached 460 ± 28.1 g/kg squalene in oily extract at 313 K/20 MPa.

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Extraction methods of Amaranthus sp. grain oil isolation

Abstract: The results of this study indicate that it is very important to choose both the right genotype and the right method of extraction for optimal oil and squalene yield. © 2015 Society of Chemical Industry.

Cited by 35 publications

References 50 publications

Plant Sources, Extraction Methods, and Uses of Squalene

Plant Sources, Extraction Methods, and Uses of Squalene

Impact of Alternaria spp. and Alternaria Toxins on Quality of Spelt Wheat

Squalene Extraction by Supercritical Fluids from Traditionally Puffed Amaranthus hypochondriacus Seeds

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